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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
23/11/2010 |
Data da última atualização: |
25/11/2010 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
VIDAL, A. M.; SOUZA, F. V. D.; SOUZA, A. da S.; COSTA, M. A. P. C. |
Afiliação: |
Ádila Melo Vidal, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; Maria Angélica Pereira de Carvalho Costa, UFRB. |
Título: |
Somatic embryogenesis in explants from cassava in vitro plants. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. v. 1, p. 228. 1 CD-ROM. |
Idioma: |
Inglês |
Notas: |
T12.006, pdf. |
Conteúdo: |
Cassava is an important food for millions of people in Africa, Asia and Latin America. Despite its great importance, this crop still has some limiting factors regarding nutritional aspects, as well as its cultivation, because some varieties are quite affected by biotic and non biotic factors. To help to overcome those obstacles, several research groups are integrating an entire scientific knowledge to generate more productive cassava varieties and to improve the existing ones. As an alternative, Biotechnology is contributing with the use of different techniques, standing out the micropropagation for the production of healthy material for propagation, providing a high number of plants in a short period of time, and the somatic embryogenesis as a system of regeneration of plants, that is a basic requirement for the improvement of a selected variety through genetic transformation. The somatic embryogenesis can be an interesting in vitro plant regeneration system for cassava. The aim of this work was to study somatic embryogenesis and in vitro plant regeneration of Cigana Preta variety from shoot tip and young leaves from in vitro plants. The explants were cultivated in MS media with 8,0 and 12,0 mg·L-1 of 2,4-D or Picloram. Two media cultures were tested to the embryos development. Embryos in cotyledonal stage were inoculated in germination media constituted of vitamins and salts of MS, 2 ?M of cupper sulfate, 2,4 g·L-1 of Phytagel®, and 1,77 ?M of BAP. The major frequency of callus and number of embryos per explant was obtained with 8 mg·L-1 of auxin Picloram. The regenerated plants presented normal development and in a minimum period of four weeks were cultivated in specific media to Micropropagation showing a normal behavior in this media. MenosCassava is an important food for millions of people in Africa, Asia and Latin America. Despite its great importance, this crop still has some limiting factors regarding nutritional aspects, as well as its cultivation, because some varieties are quite affected by biotic and non biotic factors. To help to overcome those obstacles, several research groups are integrating an entire scientific knowledge to generate more productive cassava varieties and to improve the existing ones. As an alternative, Biotechnology is contributing with the use of different techniques, standing out the micropropagation for the production of healthy material for propagation, providing a high number of plants in a short period of time, and the somatic embryogenesis as a system of regeneration of plants, that is a basic requirement for the improvement of a selected variety through genetic transformation. The somatic embryogenesis can be an interesting in vitro plant regeneration system for cassava. The aim of this work was to study somatic embryogenesis and in vitro plant regeneration of Cigana Preta variety from shoot tip and young leaves from in vitro plants. The explants were cultivated in MS media with 8,0 and 12,0 mg·L-1 of 2,4-D or Picloram. Two media cultures were tested to the embryos development. Embryos in cotyledonal stage were inoculated in germination media constituted of vitamins and salts of MS, 2 ?M of cupper sulfate, 2,4 g·L-1 of Phytagel®, and 1,77 ?M of BAP. The major frequency of c... Mostrar Tudo |
Thesagro: |
Mandioca; Propagação Vegetativa; Variedade. |
Thesaurus Nal: |
cassava; Manihot; micropropagation; varieties. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02559naa a2200253 a 4500 001 1867808 005 2010-11-25 008 2010 bl uuuu u00u1 u #d 100 1 $aVIDAL, A. M. 245 $aSomatic embryogenesis in explants from cassava in vitro plants. 260 $c2010 500 $aT12.006, pdf. 520 $aCassava is an important food for millions of people in Africa, Asia and Latin America. Despite its great importance, this crop still has some limiting factors regarding nutritional aspects, as well as its cultivation, because some varieties are quite affected by biotic and non biotic factors. To help to overcome those obstacles, several research groups are integrating an entire scientific knowledge to generate more productive cassava varieties and to improve the existing ones. As an alternative, Biotechnology is contributing with the use of different techniques, standing out the micropropagation for the production of healthy material for propagation, providing a high number of plants in a short period of time, and the somatic embryogenesis as a system of regeneration of plants, that is a basic requirement for the improvement of a selected variety through genetic transformation. The somatic embryogenesis can be an interesting in vitro plant regeneration system for cassava. The aim of this work was to study somatic embryogenesis and in vitro plant regeneration of Cigana Preta variety from shoot tip and young leaves from in vitro plants. The explants were cultivated in MS media with 8,0 and 12,0 mg·L-1 of 2,4-D or Picloram. Two media cultures were tested to the embryos development. Embryos in cotyledonal stage were inoculated in germination media constituted of vitamins and salts of MS, 2 ?M of cupper sulfate, 2,4 g·L-1 of Phytagel®, and 1,77 ?M of BAP. The major frequency of callus and number of embryos per explant was obtained with 8 mg·L-1 of auxin Picloram. The regenerated plants presented normal development and in a minimum period of four weeks were cultivated in specific media to Micropropagation showing a normal behavior in this media. 650 $acassava 650 $aManihot 650 $amicropropagation 650 $avarieties 650 $aMandioca 650 $aPropagação Vegetativa 650 $aVariedade 700 1 $aSOUZA, F. V. D. 700 1 $aSOUZA, A. da S. 700 1 $aCOSTA, M. A. P. C. 773 $tIn: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010.$gv. 1, p. 228. 1 CD-ROM.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
Data corrente: |
24/10/2014 |
Data da última atualização: |
16/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SOMAVILLA, A. L.; SONSTEGARD, T. S.; HIGA, R. H.; ROSA, A. do N.; SIQUEIRA, F.; SILVA, L. O. C. da; TORRES JUNIOR, R. A. de A.; COUTINHO, L. L.; MUDADU, M. de A.; ALENCAR, M. M. de; REGITANO, L. C. de A. |
Afiliação: |
A. L. SOMAVILLA, Programa de Pós-Graduação em Genética e Melhoramento Animal, UNESP/FCAV, Jaboticabal, Brasil; T. S. SONSTEGARD, Bovine Functional Genomics Laboratory, ANRI, USDA-ARS, Beltsville, MD, USA; ROBERTO HIROSHI HIGA, CNPTIA; ANTONIO DO NASCIMENTO ROSA, CNPGC; FABIANE SIQUEIRA, CNPGC; LUIZ OTAVIO CAMPOS DA SILVA, CNPGC; ROBERTO AUGUSTO DE A TORRES JUNIOR, CNPGC; L. L. COUTINHO, Escola Superior de Agricultura Luiz de Queiroz (Esalq), USP, Piracicaba, Brasil; MAURICIO DE ALVARENGA MUDADU, CPPSE; MAURICIO MELLO DE ALENCAR, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
Título: |
A genome-wide scan for selection signatures in Nellore cattle. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Animal Genetics, v. 45, n. 6, p. 771-781, dec. 2014 |
DOI: |
10.1111/age.12210 |
Idioma: |
Inglês |
Conteúdo: |
Brazilian Nellore cattle (Bos indicus) have been selected for growth traits for over more than four decades. In recent years, reproductive and meat quality traits have become more important because of increasing consumption, exports and consumer demand. The identification of genome regions altered by artificial selection can potentially permit a better understanding of the biology of specific phenotypes that are useful for the development of tools designed to increase selection efficiency. Therefore, the aims of this study were to detect evidence of recent selection signatures in Nellore cattle using extended haplotype homozygosity methodology and BovineHD marker genotypes (>777 000 single nucleotide polymorphisms) as well as to identify corresponding genes underlying these signals. Thirty-one significant regions (P < 0.0001) of possible recent selection signatures were detected, and 19 of these overlapped quantitative trait loci related to reproductive traits, growth, feed efficiency, meat quality, fatty acid profiles and immunity. In addition, 545 genes were identified in regions harboring selection signatures. Within this group, 58 genes were associated with growth, muscle and adipose tissue metabolism, reproductive traits or the immune system. Using relative extended haplotype homozygosity to analyze high-density single nucleotide polymorphism marker data allowed for the identification of regions potentially under artificial selection pressure in the Nellore genome, which might be used to better understand autozygosity and the effects of selection on the Nellore genome. MenosBrazilian Nellore cattle (Bos indicus) have been selected for growth traits for over more than four decades. In recent years, reproductive and meat quality traits have become more important because of increasing consumption, exports and consumer demand. The identification of genome regions altered by artificial selection can potentially permit a better understanding of the biology of specific phenotypes that are useful for the development of tools designed to increase selection efficiency. Therefore, the aims of this study were to detect evidence of recent selection signatures in Nellore cattle using extended haplotype homozygosity methodology and BovineHD marker genotypes (>777 000 single nucleotide polymorphisms) as well as to identify corresponding genes underlying these signals. Thirty-one significant regions (P < 0.0001) of possible recent selection signatures were detected, and 19 of these overlapped quantitative trait loci related to reproductive traits, growth, feed efficiency, meat quality, fatty acid profiles and immunity. In addition, 545 genes were identified in regions harboring selection signatures. Within this group, 58 genes were associated with growth, muscle and adipose tissue metabolism, reproductive traits or the immune system. Using relative extended haplotype homozygosity to analyze high-density single nucleotide polymorphism marker data allowed for the identification of regions potentially under artificial selection pressure in the Nellore genome, whic... Mostrar Tudo |
Palavras-Chave: |
Desequilíbrio de ligação; Homozigose do haplótipo estendido; Polimorfismo de nucleotídeo único; Relative extended haplotype homozygosity; Single nucleotid; Single nucleotide polymorphisms. |
Thesagro: |
Bos Indicus; Gado de corte. |
Thesaurus NAL: |
Beef cattle; Genotyping; Linkage disequilibrium; Single nucleotide polymorphism. |
Categoria do assunto: |
G Melhoramento Genético S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110576/1/PROCI-2014.00083-2.pdf
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Marc: |
LEADER 02827naa a2200397 a 4500 001 1998406 005 2022-11-16 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1111/age.12210$2DOI 100 1 $aSOMAVILLA, A. L. 245 $aA genome-wide scan for selection signatures in Nellore cattle.$h[electronic resource] 260 $c2014 520 $aBrazilian Nellore cattle (Bos indicus) have been selected for growth traits for over more than four decades. In recent years, reproductive and meat quality traits have become more important because of increasing consumption, exports and consumer demand. The identification of genome regions altered by artificial selection can potentially permit a better understanding of the biology of specific phenotypes that are useful for the development of tools designed to increase selection efficiency. Therefore, the aims of this study were to detect evidence of recent selection signatures in Nellore cattle using extended haplotype homozygosity methodology and BovineHD marker genotypes (>777 000 single nucleotide polymorphisms) as well as to identify corresponding genes underlying these signals. Thirty-one significant regions (P < 0.0001) of possible recent selection signatures were detected, and 19 of these overlapped quantitative trait loci related to reproductive traits, growth, feed efficiency, meat quality, fatty acid profiles and immunity. In addition, 545 genes were identified in regions harboring selection signatures. Within this group, 58 genes were associated with growth, muscle and adipose tissue metabolism, reproductive traits or the immune system. Using relative extended haplotype homozygosity to analyze high-density single nucleotide polymorphism marker data allowed for the identification of regions potentially under artificial selection pressure in the Nellore genome, which might be used to better understand autozygosity and the effects of selection on the Nellore genome. 650 $aBeef cattle 650 $aGenotyping 650 $aLinkage disequilibrium 650 $aSingle nucleotide polymorphism 650 $aBos Indicus 650 $aGado de corte 653 $aDesequilíbrio de ligação 653 $aHomozigose do haplótipo estendido 653 $aPolimorfismo de nucleotídeo único 653 $aRelative extended haplotype homozygosity 653 $aSingle nucleotid 653 $aSingle nucleotide polymorphisms 700 1 $aSONSTEGARD, T. S. 700 1 $aHIGA, R. H. 700 1 $aROSA, A. do N. 700 1 $aSIQUEIRA, F. 700 1 $aSILVA, L. O. C. da 700 1 $aTORRES JUNIOR, R. A. de A. 700 1 $aCOUTINHO, L. L. 700 1 $aMUDADU, M. de A. 700 1 $aALENCAR, M. M. de 700 1 $aREGITANO, L. C. de A. 773 $tAnimal Genetics$gv. 45, n. 6, p. 771-781, dec. 2014
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Embrapa Pecuária Sudeste (CPPSE) |
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