02559naa a2200253 a 450000100080000000500110000800800410001910000170006024500680007726000090014550000180015452017780017265000120195065000120196265000210197465000140199565000130200965000280202265000140205070000200206470000200208470000230210477301780212718678082010-11-25       2010    bl uuuu     u00u1 u    #d1 aVIDAL, A. M.  aSomatic embryogenesis in explants from cassava in vitro plants.  c2010  aT12.006, pdf.  aCassava is an important food for millions of people in Africa, Asia and Latin America. Despite its great importance, this crop still has some limiting factors regarding nutritional aspects, as well as its cultivation, because some varieties are quite affected by biotic and non biotic factors. To help to overcome those obstacles, several research groups are integrating an entire scientific knowledge to generate more productive cassava varieties and to improve the existing ones. As an alternative, Biotechnology is contributing with the use of different techniques, standing out the micropropagation for the production of healthy material for propagation, providing a high number of plants in a short period of time, and the somatic embryogenesis as a system of regeneration of plants, that is a basic requirement for the improvement of a selected variety through genetic transformation. The somatic embryogenesis can be an interesting in vitro plant regeneration system for cassava. The aim of this work was to study somatic embryogenesis and in vitro plant regeneration of Cigana Preta variety from shoot tip and young leaves from in vitro plants. The explants were cultivated in MS media with 8,0 and 12,0 mg·L-1 of 2,4-D or Picloram. Two media cultures were tested to the embryos development. Embryos in cotyledonal stage were inoculated in germination media constituted of vitamins and salts of MS, 2 ?M of cupper sulfate, 2,4 g·L-1 of Phytagel®, and 1,77 ?M of BAP. The major frequency of callus and number of embryos per explant was obtained with 8 mg·L-1 of auxin Picloram. The regenerated plants presented normal development and in a minimum period of four weeks were cultivated in specific media to Micropropagation showing a normal behavior in this media.  acassava  aManihot  amicropropagation  avarieties  aMandioca  aPropagação Vegetativa  aVariedade1 aSOUZA, F. V. D.1 aSOUZA, A. da S.1 aCOSTA, M. A. P. C.  tIn: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010.gv. 1, p. 228. 1 CD-ROM.