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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/08/2021 |
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Data da última atualização: |
13/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; FEUCHARD, V. L. da S.; FREITAS, C. de; ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SARAIVA, N. Z. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; VIVIANE LUZIA DA SILVA FEUCHARD, Pesagro; CELIO DE FREITAS, CNPGL; PAOLA MARIA DA SILVA ROSA; AGOSTINHO JORGE DOS REIS CAMARGO, Pesagro; NAIARA ZOCCAL SARAIVA, CNPGL. |
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Título: |
In-straw warming protocol improves survival of vitrified embryos and allows direct transfer in cattle. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Cryobiology, v. 97, p. 222-225, 2020. |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2020.02.007 |
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Idioma: |
Inglês |
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Conteúdo: |
Vitrification is a superior method for cryopreservation of IVF embryos, but due to complicated warming protocols, it is not commonly used for commercial bovine embryos routine. To overcome the need of laboratory embryo preparation during warming, we developed an in-straw warming protocol compatible with most vitrification devices for embryo transfer without sucrose gradient steps and embryo evaluation. Surprisingly, one of the tested protocols improved embryo survival (95.0%* vs 83.1% expansion rate and 74.2%* vs 51.5% hatching rate) compared to conventional in-plate warming. Embryo quality was also increased, taken by the higher total cell numbers (160.7 ± 8.6* vs 99.0 ± 7.9) and lower apoptosis index (4.9 ±0.6* vs 11.5 ± 2.4) 48 h after warming. Pregnancy rates were similar between vitrified-warmed embryos and fresh embryos (40% vs 43%). Based on our results, we suggest in-straw warming should always be used for vitrified embryos due to beneficial effects. Direct transfer can be safely performed using this protocol. |
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Palavras-Chave: |
Criotolerância; Fertilização in vitro; Vitrificação. |
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Thesagro: |
Bovino; Criopreservação; Embrião Animal; Palha; Reprodução Animal; Sacarose. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01940naa a2200301 a 4500
001 2133567
005 2021-08-13
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2020.02.007$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aIn-straw warming protocol improves survival of vitrified embryos and allows direct transfer in cattle.$h[electronic resource]
260 $c2020
520 $aVitrification is a superior method for cryopreservation of IVF embryos, but due to complicated warming protocols, it is not commonly used for commercial bovine embryos routine. To overcome the need of laboratory embryo preparation during warming, we developed an in-straw warming protocol compatible with most vitrification devices for embryo transfer without sucrose gradient steps and embryo evaluation. Surprisingly, one of the tested protocols improved embryo survival (95.0%* vs 83.1% expansion rate and 74.2%* vs 51.5% hatching rate) compared to conventional in-plate warming. Embryo quality was also increased, taken by the higher total cell numbers (160.7 ± 8.6* vs 99.0 ± 7.9) and lower apoptosis index (4.9 ±0.6* vs 11.5 ± 2.4) 48 h after warming. Pregnancy rates were similar between vitrified-warmed embryos and fresh embryos (40% vs 43%). Based on our results, we suggest in-straw warming should always be used for vitrified embryos due to beneficial effects. Direct transfer can be safely performed using this protocol.
650 $aBovino
650 $aCriopreservação
650 $aEmbrião Animal
650 $aPalha
650 $aReprodução Animal
650 $aSacarose
653 $aCriotolerância
653 $aFertilização in vitro
653 $aVitrificação
700 1 $aFEUCHARD, V. L. da S.
700 1 $aFREITAS, C. de
700 1 $aROSA, P. M. da S.
700 1 $aCAMARGO, A. J. dos R.
700 1 $aSARAIVA, N. Z.
773 $tCryobiology$gv. 97, p. 222-225, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
30/11/2015 |
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Data da última atualização: |
08/03/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
DEVINCENZI, T.; NABINGER, C.; GENRO, T. C. M.; NALERIO, E.; JUCHEM, S.; FEDRIGO, J. K.; PINTO, M. F. |
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Afiliação: |
Thais Devincenzi, UFRGS; Carlos Nabinger, UFRGS; TERESA CRISTINA MORAES GENRO, CPPSUL; ELEN SILVEIRA NALERIO, CPPSUL; SERGIO DE OLIVEIRA JUCHEM, CPPSUL; Jean Kassio Fedrigo, UFRGS; Marcelo Fett Pinto, UFRGS. |
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Título: |
Meat quality from grazing-based beef production systems on natural grasslands os Pampa biome. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY, 61., 2015, Clermont-Ferrand. Proceedings... Clermont-Ferrand: INRA, 2015. |
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Idioma: |
Inglês |
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Notas: |
ICOMST. |
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Conteúdo: |
Producing beef on natural grasslands may represent an alternative to local farmers to add value on meat products of Pampa Biome. However, the quality characteristics of these products must be investigated. In this sense, we investigated the meat quality of three grazing -based beef production systems in Pampa biome: Natural grassland (NG), Improved natural grassland (ING, fertilized and oversown with winter species) and Sorghum pasture (SP). |
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Thesagro: |
Ácido graxo; Carne; Gado de corte; Pastagem. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01202nam a2200241 a 4500
001 2029986
005 2016-03-08
008 2015 bl uuuu u00u1 u #d
100 1 $aDEVINCENZI, T.
245 $aMeat quality from grazing-based beef production systems on natural grasslands os Pampa biome.$h[electronic resource]
260 $aIn: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY, 61., 2015, Clermont-Ferrand. Proceedings... Clermont-Ferrand: INRA$c2015
500 $aICOMST.
520 $aProducing beef on natural grasslands may represent an alternative to local farmers to add value on meat products of Pampa Biome. However, the quality characteristics of these products must be investigated. In this sense, we investigated the meat quality of three grazing -based beef production systems in Pampa biome: Natural grassland (NG), Improved natural grassland (ING, fertilized and oversown with winter species) and Sorghum pasture (SP).
650 $aÁcido graxo
650 $aCarne
650 $aGado de corte
650 $aPastagem
700 1 $aNABINGER, C.
700 1 $aGENRO, T. C. M.
700 1 $aNALERIO, E.
700 1 $aJUCHEM, S.
700 1 $aFEDRIGO, J. K.
700 1 $aPINTO, M. F.
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Embrapa Pecuária Sul (CPPSUL) |
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