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| 2. |  | CUNHA, G. R.; HAAS, J. C.; BERLATO, M. A. (ed.). Applications of climate forecasting for better decision-making processes in agriculture. Passo Fundo: Embrapa Trigo, 2001. 327 p. The contribution from the 12th Regional Climate Outlook Forum for Southeastern South , America, held in Passo Fundo, RS, Brazil, April 24-25, 2001.| Biblioteca(s): Embrapa Agrobiologia; Embrapa Amazônia Ocidental; Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
31/03/1993 |
|
Data da última atualização: |
01/07/2024 |
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Autoria: |
RESENDE, R. de O.; AVILA, A. C. de; GOLDBACH, R. W.; PETERS, D. |
|
Afiliação: |
EMBRAPA-CNPH; ANTONIO CARLOS DE AVILA, CNPH. |
|
Título: |
Comparison of polyclonal antisera in the detection of tomato spotted wilt virus using the double antibody sandwich and cocktail ELISA. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Journal of Phytopathology, v.132, p.46-56, 1991. |
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DOI: |
https://doi.org/10.1111/j.1439-0434.1991.tb00092.x |
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Idioma: |
Inglês |
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Conteúdo: |
Different polyclonal antisera and enzyme-linked immunosorbent assay (ELISA) procedures have been tested for their potential to detect tomato spotted wilt virus (TSWV). The virus could efficiently be detected in high dilutions of sap from infected plants, and at low concentrations of purified virus and nucleocapsid protein preparations in the cocktail ELISA and the double antibody sandwich ELISA (DAS-ELISA). Amounts of 1 to 3 ng of virus protein still gave positive readings using purified preparations, while sap could be diluted approximately 100,000 times. Differences in the detection level were observed using nucleocapsid protein antiserum (anti-N-serum) and the antiserum against intact virus particles (anti-TSWV-serum), but both antisera showed to be powerful sera for the detection of TSWV. Using anti-N-serum, TSWV could be detected in highly diluted extracts of different hosts, and also in leaf extracts or intact tissues stored for 30 days under different conditions. These results indicate that the TSWV nucleocapsid protein remains antigenic for long periods. |
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Palavras-Chave: |
Deteccao; Spotted Wilt Virus; Tomato; TSWV; Vira-Cabeca. |
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Thesagro: |
Elisa; Tomate. |
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Thesaurus NAL: |
detection. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01854naa a2200265 a 4500
001 1749566
005 2024-07-01
008 1991 bl --- 0-- u #d
024 7 $ahttps://doi.org/10.1111/j.1439-0434.1991.tb00092.x$2DOI
100 1 $aRESENDE, R. de O.
245 $aComparison of polyclonal antisera in the detection of tomato spotted wilt virus using the double antibody sandwich and cocktail ELISA.
260 $c1991
520 $aDifferent polyclonal antisera and enzyme-linked immunosorbent assay (ELISA) procedures have been tested for their potential to detect tomato spotted wilt virus (TSWV). The virus could efficiently be detected in high dilutions of sap from infected plants, and at low concentrations of purified virus and nucleocapsid protein preparations in the cocktail ELISA and the double antibody sandwich ELISA (DAS-ELISA). Amounts of 1 to 3 ng of virus protein still gave positive readings using purified preparations, while sap could be diluted approximately 100,000 times. Differences in the detection level were observed using nucleocapsid protein antiserum (anti-N-serum) and the antiserum against intact virus particles (anti-TSWV-serum), but both antisera showed to be powerful sera for the detection of TSWV. Using anti-N-serum, TSWV could be detected in highly diluted extracts of different hosts, and also in leaf extracts or intact tissues stored for 30 days under different conditions. These results indicate that the TSWV nucleocapsid protein remains antigenic for long periods.
650 $adetection
650 $aElisa
650 $aTomate
653 $aDeteccao
653 $aSpotted Wilt Virus
653 $aTomato
653 $aTSWV
653 $aVira-Cabeca
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R. W.
700 1 $aPETERS, D.
773 $tJournal of Phytopathology$gv.132, p.46-56, 1991.
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