01877naa a2200265 a 450000100080000000500110000800800410001902400600006010000220012024501620014226000090030452010830031365000140139665000100141065000110142065300130143165300230144465300110146765300090147865300160148770000200150370000200152370000150154377300530155817495662026-01-19       1991    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1111/j.1439-0434.1991.tb00092.x2DOI1 aRESENDE, R. de O.  aComparison of polyclonal antisera in the detection of tomato spotted wilt virus using the double antibody sandwich and cocktail ELISA.h[electronic resource]  c1991  aDifferent polyclonal antisera and enzyme-linked immunosorbent assay (ELISA) procedures have been tested for their potential to detect tomato spotted wilt virus (TSWV). The virus could efficiently be detected in high dilutions of sap from infected plants, and at low concentrations of purified virus and nucleocapsid protein preparations in the cocktail ELISA and the double antibody sandwich ELISA (DAS-ELISA). Amounts of 1 to 3 ng of virus protein still gave positive readings using purified preparations, while sap could be diluted approximately 100,000 times. Differences in the detection level were observed using nucleocapsid protein antiserum (anti-N-serum) and the antiserum against intact virus particles (anti-TSWV-serum), but both antisera showed to be powerful sera for the detection of TSWV. Using anti-N-serum, TSWV could be detected in highly diluted extracts of different hosts, and also in leaf extracts or intact tissues stored for 30 days under different conditions. These results indicate that the TSWV nucleocapsid protein remains antigenic for long periods.  aDetection  aElisa  aTomate  aDeteccao  aSpotted Wilt Virus  aTomato  aTSWV  aVira-Cabeca1 aAVILA, A. C. de1 aGOLDBACH, R. W.1 aPETERS, D.  tJournal of Phytopathologygv.132, p.46-56, 1991.