|
|
Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
08/07/2015 |
Data da última atualização: |
03/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PINTO, M. dos S. T.; RIBEIRO, J. M.; ARAUJO, F. P. de; MELO, N. F. de; FERNANDES, K. V. S. |
Afiliação: |
MARCIO DOS SANTOS TEIXEIRA PINTO, Universidade Federal do Tocantins - UFT; JULIANA MARTINS RIBEIRO, CPATSA; FRANCISCO PINHEIRO DE ARAUJO, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA; KÁTIA VALEVSKI SALES FERNANDES, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF. |
Título: |
Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.). |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
African Journal of Biotechnology, v. 14, n. 21, p. 1838-1845, 2015. |
DOI: |
10.5897/AJB2015.14521 |
Idioma: |
Inglês |
Conteúdo: |
Umbu plants are drought resistant trees which are able to store water and several other substances into its adapted root, named xylopodium. The exsudate from xilopodium is a natural solution rich in salts, sugars and a little concentration of proteins. In this work, we report the purification of a peroxidase (POX) from umbu xilopodium exsudate by direct extraction from polyacrylamide electrophoresis gels. Umbu POX showed optimum activity at pHs around 6.0 to 7.0 and high thermal resistance after incubation at 7 0°C for 6 min. POX activity present in crude extracts was more heat - resistant than in its purified form. When assayed with metal ions, umbu POX activity was shown to be inhibited by Mn 2+ and stimulated by Ca 2+ and Mg 2+ ; it was also inhibited by sodium azid e (concentrations higher then 1 mM) and not inhibited by either EDTA or tropolone. POX Km values for guaiacol and methylcatechol substrates were 6.83 and 22.25, respectively. The enzyme is proposed to be a guaiacol peroxidase and was seen to be located at higher concentrations in the outermost layers of xylopodium tissue, such as the endoderm. |
Palavras-Chave: |
Guaiacol peroxidase; Root enzyme; Spondia tuberosa; Tssue brownig. |
Thesagro: |
Caatinga; Peroxidase; Umbu. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/126327/1/Juliana-2015.pdf
|
Marc: |
LEADER 01940naa a2200265 a 4500 001 2019494 005 2017-05-03 008 2015 bl uuuu u00u1 u #d 024 7 $a10.5897/AJB2015.14521$2DOI 100 1 $aPINTO, M. dos S. T. 245 $aPurification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.).$h[electronic resource] 260 $c2015 520 $aUmbu plants are drought resistant trees which are able to store water and several other substances into its adapted root, named xylopodium. The exsudate from xilopodium is a natural solution rich in salts, sugars and a little concentration of proteins. In this work, we report the purification of a peroxidase (POX) from umbu xilopodium exsudate by direct extraction from polyacrylamide electrophoresis gels. Umbu POX showed optimum activity at pHs around 6.0 to 7.0 and high thermal resistance after incubation at 7 0°C for 6 min. POX activity present in crude extracts was more heat - resistant than in its purified form. When assayed with metal ions, umbu POX activity was shown to be inhibited by Mn 2+ and stimulated by Ca 2+ and Mg 2+ ; it was also inhibited by sodium azid e (concentrations higher then 1 mM) and not inhibited by either EDTA or tropolone. POX Km values for guaiacol and methylcatechol substrates were 6.83 and 22.25, respectively. The enzyme is proposed to be a guaiacol peroxidase and was seen to be located at higher concentrations in the outermost layers of xylopodium tissue, such as the endoderm. 650 $aCaatinga 650 $aPeroxidase 650 $aUmbu 653 $aGuaiacol peroxidase 653 $aRoot enzyme 653 $aSpondia tuberosa 653 $aTssue brownig 700 1 $aRIBEIRO, J. M. 700 1 $aARAUJO, F. P. de 700 1 $aMELO, N. F. de 700 1 $aFERNANDES, K. V. S. 773 $tAfrican Journal of Biotechnology$gv. 14, n. 21, p. 1838-1845, 2015.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Semiárido (CPATSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agropecuária Oeste. Para informações adicionais entre em contato com cpao.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agropecuária Oeste. |
Data corrente: |
27/03/2000 |
Data da última atualização: |
04/10/2001 |
Autoria: |
FERNANDES, F. M.; HECKLER, J. C. |
Título: |
Soja em rotacao com algodoeiro e milho no sistema plantio direto. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 21., 1999, Dourados. Resumos... Dourados: Embrapa Agropecuaria Oeste; Londrina: Embrapa Soja, 1999. p.50. (Embrapa Agropecuaria Oeste. Documentos, 7; Embrapa Soja. Documentos, 134). |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Direct sowing; Dourados; Maize; Mato Grosso; Mato Grosso do Sul; Rotational cropping; Soybean. |
Thesagro: |
Algodão; Cerrado; Glycine Max; Gossypium Hirsutum; Milho; Plantio Direto; Rotação de Cultura; Soja; Zea Mays. |
Thesaurus NAL: |
Brazil; cotton; Gossypium; soybeans. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01116naa a2200373 a 4500 001 1243843 005 2001-10-04 008 1999 bl uuuu u00u1 u #d 100 1 $aFERNANDES, F. M. 245 $aSoja em rotacao com algodoeiro e milho no sistema plantio direto. 260 $c1999 650 $aBrazil 650 $acotton 650 $aGossypium 650 $asoybeans 650 $aAlgodão 650 $aCerrado 650 $aGlycine Max 650 $aGossypium Hirsutum 650 $aMilho 650 $aPlantio Direto 650 $aRotação de Cultura 650 $aSoja 650 $aZea Mays 653 $aBrasil 653 $aDirect sowing 653 $aDourados 653 $aMaize 653 $aMato Grosso 653 $aMato Grosso do Sul 653 $aRotational cropping 653 $aSoybean 700 1 $aHECKLER, J. C. 773 $tIn: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 21., 1999, Dourados. Resumos... Dourados: Embrapa Agropecuaria Oeste; Londrina: Embrapa Soja, 1999. p.50. (Embrapa Agropecuaria Oeste. Documentos, 7; Embrapa Soja. Documentos, 134).
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|