01940naa a2200265 a 450000100080000000500110000800800410001902400310006010000240009124501480011526000090026352011310027265000130140365000150141665000090143165300240144065300160146465300210148065300180150170000190151970000210153870000190155970000240157877300720160220194942017-05-03       2015    bl uuuu     u00u1 u    #d7 a10.5897/AJB2015.145212DOI1 aPINTO, M. dos S. T.  aPurification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.).h[electronic resource]  c2015  aUmbu plants are drought resistant trees which are able to store water and several other substances into its adapted root, named xylopodium. The exsudate from xilopodium is a natural solution rich in salts, sugars and a little concentration of proteins. In this work, we report the purification of a peroxidase (POX) from umbu xilopodium exsudate by direct extraction from polyacrylamide electrophoresis gels. Umbu POX showed optimum activity at pHs around 6.0 to 7.0 and high thermal resistance after incubation at 7 0°C for 6 min. POX activity present in crude extracts was more heat - resistant than in its purified form. When assayed with metal ions, umbu POX activity was shown to be inhibited by Mn 2+ and stimulated by Ca 2+ and Mg 2+ ; it was also inhibited by sodium azid e (concentrations higher then 1 mM) and not inhibited by either EDTA or tropolone. POX Km values for guaiacol and methylcatechol substrates were 6.83 and 22.25, respectively. The enzyme is proposed to be a guaiacol peroxidase and was seen to be located at higher concentrations in the outermost layers of xylopodium tissue, such as the endoderm.  aCaatinga  aPeroxidase  aUmbu  aGuaiacol peroxidase  aRoot enzyme  aSpondia tuberosa  aTssue brownig1 aRIBEIRO, J. M.1 aARAUJO, F. P. de1 aMELO, N. F. de1 aFERNANDES, K. V. S.  tAfrican Journal of Biotechnologygv. 14, n. 21, p. 1838-1845, 2015.