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| 3. |  | HARTMAN, M.; BOLSANELLO, R. X.; DOMINGUES, P. F.; MELLO JÚNIOR, A. S. de; LANGONI, H. Efeito da mastite sobre a contagem de células somáticas (CC) em ovelhas da raça Bergamácia. Veterinária e Zootecnia, Botucatu, v. 16, n. 1, p. 213-220, mar. 2009.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 5. | | RIBEIRO, M. G.; COSTA, E. O.; ROCHA, N. S.; DOMINGUES, P. F.; RIBEIRO, A. R.; NARDI JUNIOR, G. Citologia aspirativa com agulha fina e microscopia eletrônica de varredura no diagnóstico de mastite clínica bovina por Prototheca zopfii. Revista Napgama, v. 2, n. 5, p. 15-20, 1999.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
20/11/2019 |
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Data da última atualização: |
20/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NUNES, M. Z.; BERNARDI, D.; BARONIO, C. A.; PASINATO, J.; BALDIN, M. M.; BOTTON, M. |
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Afiliação: |
Marcelo Z. Nunes, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; Daniel Bernardi, Dept of Entomology, Embrapa Clima Temperado, Pelotas, RS, Brazil; Cléber A. Baronio, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil; Joel Pasinato, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, Brazil; Morgana M. Baldin, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, Brazil; MARCOS BOTTON, CNPUV. |
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Título: |
A laboratory bioassay method to assess the use of Toxic bait on anastrepha fraterculus (Weidemann 1830). |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Neotropical Entomology, n. 49, p. 124-130, 2020. |
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DOI: |
https://doi.org/10.1007/s13744-019-00728-y |
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Idioma: |
Português |
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Conteúdo: |
The lack of standardization of bioassays for the evaluation of toxic baittoxicity on the South American fruit fly,Anastrepha fraterculus(Weidemann 1830), has led to erroneous interpretations of assay results.The objective of this study was to develop a methodology for the stan-dardization and validation of toxicological tests onA. fraterculustoxic baitusing the Success?0.02CB formulation (80 mg L−1of spinosad).Anastrepha fraterculusadults, obtained from larvae reared on an artificialdiet, showed higher susceptibility (LT50= 48.96 h) than adults from larvaereared on cattley guava (LT50= 53.83 h) and mango fruit (LT50= 53.55 h).Anastrepha fraterculusadults at the age of five (LT50= 65.30 h), 15 (LT50=59.01 h), and 30 (LT50= 55.53 h) days presented similar toxicity. Theconsumption of toxic bait (4.74 mg) increased at 15 days, a fact alsoobserved with insects without food deprivation. In addition, the absenceof a food source (artificial diet) with the toxic bait significantly reducedadult mortality time by 7 h (LT50= 57.42 h). In relation to exposure time,adults exposed to toxic bait for 1 h reduced consumption by 25%; however,they showed the same susceptibility as insects exposed to 2 (LT50=55.72h), 4 (LT50=57.64h),and8h(LT50= 57.76 h). However, with 24 h of fooddeprivation, they had a higher susceptibility (LT50= 46.48 h). Five-day-oldA. fraterculusadults fed an artificial diet before being deprived of food for12 or 24 h, then exposed to toxic bait for 4 h in the absence of a foodsource, are considered optimum conditions to evaluate the toxicity oftoxic bait. MenosThe lack of standardization of bioassays for the evaluation of toxic baittoxicity on the South American fruit fly,Anastrepha fraterculus(Weidemann 1830), has led to erroneous interpretations of assay results.The objective of this study was to develop a methodology for the stan-dardization and validation of toxicological tests onA. fraterculustoxic baitusing the Success?0.02CB formulation (80 mg L−1of spinosad).Anastrepha fraterculusadults, obtained from larvae reared on an artificialdiet, showed higher susceptibility (LT50= 48.96 h) than adults from larvaereared on cattley guava (LT50= 53.83 h) and mango fruit (LT50= 53.55 h).Anastrepha fraterculusadults at the age of five (LT50= 65.30 h), 15 (LT50=59.01 h), and 30 (LT50= 55.53 h) days presented similar toxicity. Theconsumption of toxic bait (4.74 mg) increased at 15 days, a fact alsoobserved with insects without food deprivation. In addition, the absenceof a food source (artificial diet) with the toxic bait significantly reducedadult mortality time by 7 h (LT50= 57.42 h). In relation to exposure time,adults exposed to toxic bait for 1 h reduced consumption by 25%; however,they showed the same susceptibility as insects exposed to 2 (LT50=55.72h), 4 (LT50=57.64h),and8h(LT50= 57.76 h). However, with 24 h of fooddeprivation, they had a higher susceptibility (LT50= 46.48 h). Five-day-oldA. fraterculusadults fed an artificial diet before being deprived of food for12 or 24 h, then exposed to toxic bait for 4 h in the absence... Mostrar Tudo |
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Palavras-Chave: |
Frui fly; South American fruit fly. |
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Thesaurus NAL: |
Methodology; Spinosad. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1114737/1/Nunesetal2019ALaboratoryBioassayMethodtoAssesstheUseofToxic.pdf
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Marc: |
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Embrapa Uva e Vinho (CNPUV) |
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