02316naa a2200241 a 450000100080000000500110000800800410001902400520006010000170011224501320012926000090026152015940027065000160186465000130188065300130189365300290190670000170193570000190195270000170197170000180198870000150200677300530202121147372020-11-20       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s13744-019-00728-y2DOI1 aNUNES, M. Z.  aA laboratory bioassay method to assess the use of Toxic bait on anastrepha fraterculus (Weidemann 1830).h[electronic resource]  c2020  aThe lack of standardization of bioassays for the evaluation of toxic baittoxicity on the South American fruit fly,Anastrepha fraterculus(Weidemann 1830), has led to erroneous interpretations of assay results.The objective of this study was to develop a methodology for the stan-dardization and validation of toxicological tests onA. fraterculustoxic baitusing the Success?0.02CB formulation (80 mg L−1of spinosad).Anastrepha fraterculusadults, obtained from larvae reared on an artificialdiet, showed higher susceptibility (LT50= 48.96 h) than adults from larvaereared on cattley guava (LT50= 53.83 h) and mango fruit (LT50= 53.55 h).Anastrepha fraterculusadults at the age of five (LT50= 65.30 h), 15 (LT50=59.01 h), and 30 (LT50= 55.53 h) days presented similar toxicity. Theconsumption of toxic bait (4.74 mg) increased at 15 days, a fact alsoobserved with insects without food deprivation. In addition, the absenceof a food source (artificial diet) with the toxic bait significantly reducedadult mortality time by 7 h (LT50= 57.42 h). In relation to exposure time,adults exposed to toxic bait for 1 h reduced consumption by 25%; however,they showed the same susceptibility as insects exposed to 2 (LT50=55.72h), 4 (LT50=57.64h),and8h(LT50= 57.76 h). However, with 24 h of fooddeprivation, they had a higher susceptibility (LT50= 46.48 h). Five-day-oldA. fraterculusadults fed an artificial diet before being deprived of food for12 or 24 h, then exposed to toxic bait for 4 h in the absence of a foodsource, are considered optimum conditions to evaluate the toxicity oftoxic bait.  aMethodology  aSpinosad  aFrui fly  aSouth American fruit fly1 aBERNARDI, D.1 aBARONIO, C. A.1 aPASINATO, J.1 aBALDIN, M. M.1 aBOTTON, M.  tNeotropical Entomologygn. 49, p. 124-130, 2020.