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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
15/02/2016 |
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Data da última atualização: |
26/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FANÉ, R.; TRAORÉ, D.; KASSOGUÉ, A.; SAMALÉ, F.; DICKO, A. H.; HAMADOUN, A.; VALICENTE, F. H.; BABANA, A. H. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Genetic diversity of native Bacillus thuringiensis strains isolated from soil of different localities in Mali and their cry gene profile. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
British Biotechnology Journal, v. 12, n. 1, p. 1-8, 2016. |
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DOI: |
10.9734/BBJ/2016/20432 |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of our collection, the cry2 gene was always present with one or two cry1 sub-group(s). Conclusion: In this study, high genetic diversity in the native Bt strains from the bacterial collection in Mali was observed. Most of the native Bt strains studied harbor the cry1 gene only. In the cry1 gene profile, the cry1F sub-group was found to be the most frequently detected. None of studied Bt strains harbored the cry2 gene only. MenosAims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of o... Mostrar Tudo |
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Palavras-Chave: |
Helicoverpa armegira. |
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Thesagro: |
Bacillus thuringiensis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02685naa a2200241 a 4500
001 2036996
005 2017-09-26
008 2016 bl uuuu u00u1 u #d
024 7 $a10.9734/BBJ/2016/20432$2DOI
100 1 $aFANÉ, R.
245 $aGenetic diversity of native Bacillus thuringiensis strains isolated from soil of different localities in Mali and their cry gene profile.$h[electronic resource]
260 $c2016
520 $aAims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of our collection, the cry2 gene was always present with one or two cry1 sub-group(s). Conclusion: In this study, high genetic diversity in the native Bt strains from the bacterial collection in Mali was observed. Most of the native Bt strains studied harbor the cry1 gene only. In the cry1 gene profile, the cry1F sub-group was found to be the most frequently detected. None of studied Bt strains harbored the cry2 gene only.
650 $aBacillus thuringiensis
653 $aHelicoverpa armegira
700 1 $aTRAORÉ, D.
700 1 $aKASSOGUÉ, A.
700 1 $aSAMALÉ, F.
700 1 $aDICKO, A. H.
700 1 $aHAMADOUN, A.
700 1 $aVALICENTE, F. H.
700 1 $aBABANA, A. H.
773 $tBritish Biotechnology Journal$gv. 12, n. 1, p. 1-8, 2016.
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Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
31/10/2019 |
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Data da última atualização: |
27/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARINHO, R. das G. B.; DAMASCENO, L. F.; CASTELO, A. S.; ROCHA, J. D. M.; YOSHIOKA, E. T. O. |
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Afiliação: |
RENATA DAS GRAÇAS BARBOSA MARINHO, PESCAP; LEANDRO FERNANDES DAMASCENO, CPAF-AP; ANTONIELSON SILVA CASTELO, AGROINDÚSTRIA CUNHAÚ; JOANA D'ARC MAURICIO ROCHA, CIFPA; ELIANE TIE OBA YOSHIOKA, CPAF-AP. |
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Título: |
Produção de silagem biológica de pescado com abacaxi. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: JORNADA CIENTÍFICA DA EMBRAPA AMAPÁ, 4., 2018, Macapá. Resumos... Macapá: Embrapa Amapá, 2019. p. 28. Editores técnicos: Adilson Lopes Lima e Ricardo Adaime. |
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Idioma: |
Português |
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Conteúdo: |
A produção de silagem biológica requer o emprego de baixa tecnologia, reduzindo os custos e gerando um produto de alto valor biológico com potencialidade para uso na nutrição de organismos aquáticos. O presente estudo objetivou avaliar diferentes silagens biológicas de pescado com abacaxi. O experimento foi realizado no Laboratório de Nutrição de Organismos Aquáticos da Embrapa Amapá por sete dias. O fermento utilizado foi constituído de repolho, abacaxi, farinha de trigo, sal, vinagre e Butil Hidroxitolueno (BHT) como antioxidante. |
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Thesagro: |
Alimento Animal; Nutrição Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1113707/1/CPAFAP2019Producaosilagem.pdf
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Marc: |
LEADER 01212nam a2200181 a 4500
001 2113707
005 2020-01-27
008 2019 bl uuuu u00u1 u #d
100 1 $aMARINHO, R. das G. B.
245 $aProdução de silagem biológica de pescado com abacaxi.$h[electronic resource]
260 $aIn: JORNADA CIENTÍFICA DA EMBRAPA AMAPÁ, 4., 2018, Macapá. Resumos... Macapá: Embrapa Amapá, 2019. p. 28. Editores técnicos: Adilson Lopes Lima e Ricardo Adaime.$c2019
520 $aA produção de silagem biológica requer o emprego de baixa tecnologia, reduzindo os custos e gerando um produto de alto valor biológico com potencialidade para uso na nutrição de organismos aquáticos. O presente estudo objetivou avaliar diferentes silagens biológicas de pescado com abacaxi. O experimento foi realizado no Laboratório de Nutrição de Organismos Aquáticos da Embrapa Amapá por sete dias. O fermento utilizado foi constituído de repolho, abacaxi, farinha de trigo, sal, vinagre e Butil Hidroxitolueno (BHT) como antioxidante.
650 $aAlimento Animal
650 $aNutrição Animal
700 1 $aDAMASCENO, L. F.
700 1 $aCASTELO, A. S.
700 1 $aROCHA, J. D. M.
700 1 $aYOSHIOKA, E. T. O.
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Embrapa Amapá (CPAF-AP) |
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