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| 2. |  | MALOSSI, C. D.; CAVALCANTE, R. V.; ULLMANN, L. S.; NOGUEIRA, M. F.; AGUIAR, D. M.; KROON, E. G.; ARAUJO JR. J. P. Identification of a novel equine infectious anemia virus strain by massive sequencing in an endemic zone in Pantanal, Brazil. Journal of the Brazilian Society for Virology, v. 19, p.259-260, 2014. Supplement 2. BRAZILIAN CONGRESS OF VIROLOGY, 25.; MERCOSUR MEETING OF VIROLOGY, 9., 2014, Ribeirão Preto, SP.| Biblioteca(s): Embrapa Pantanal. |
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
06/12/2013 |
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Data da última atualização: |
15/04/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CAVALCANTE, R. V.; NOGUEIRA, M. F.; ARAÚJO Jr. J. P. |
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Afiliação: |
MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP. |
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Título: |
Evaluation of nested and semi nested PCR in detection of equine infectious anemia virus infection in horses of Pantanal region, Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Virus Reviews & Research, v. 18, p. 219, 2013. Supplement 1. |
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Idioma: |
Inglês |
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Notas: |
Trabalho apresentado no XXIV Congresso Brasileiro de Virologia & VIII Reunião do Mercosul de Virologia, Porto Seguro, BA. |
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Conteúdo: |
Equine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results.
Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques. |
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Palavras-Chave: |
Anemia Infecciosa Equina; EIA; EIAV (Equine infectious anemia virus); Equino; Retrovirus. |
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Thesagro: |
Virus. |
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Thesaurus NAL: |
Epidemiology; Equine infectious anemia; Horse diseases. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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URL: |
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/164439/1/VV218.pdf
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Marc: |
LEADER 01654naa a2200265 a 4500
001 2076399
005 2019-04-15
008 2013 bl --- 0-- u #d
100 1 $aCAVALCANTE, R. V.
245 $aEvaluation of nested and semi nested PCR in detection of equine infectious anemia virus infection in horses of Pantanal region, Brazil.
260 $c2013
500 $aTrabalho apresentado no XXIV Congresso Brasileiro de Virologia & VIII Reunião do Mercosul de Virologia, Porto Seguro, BA.
520 $aEquine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results. Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques.
650 $aEpidemiology
650 $aEquine infectious anemia
650 $aHorse diseases
650 $aVirus
653 $aAnemia Infecciosa Equina
653 $aEIA
653 $aEIAV (Equine infectious anemia virus)
653 $aEquino
653 $aRetrovirus
700 1 $aNOGUEIRA, M. F.
700 1 $aARAÚJO Jr. J. P.
773 $tVirus Reviews & Research$gv. 18, p. 219, 2013. Supplement 1.
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Registro original: |
Embrapa Pantanal (CPAP) |
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