01615nam a2200229 a 450000100080000000500110000800800410001910000220006024501630008226000710024550001270031652007420044365000170118565000290120265000190123165000120125065000100126265300290127265300420130170000200134370000220136320763992019-04-15       2013    bl uuuu     u00u1 u    #d1 aCAVALCANTE, R. V.  aEvaluation of nested and semi nested PCR in detection of equine infectious anemia virus infection in horses of Pantanal region, Brazil.h[electronic resource]  aVirus Reviews & Research, v. 18, p. 219, 2013. Supplement 1.c2013  aTrabalho apresentado no XXIV Congresso Brasileiro de Virologia & VIII Reunião do Mercosul de Virologia, Porto Seguro, BA.  aEquine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results. Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques.  aEpidemiology  aEquine infectious anemia  aHorse diseases  aEqüino  aVirus  aAnemia Infecciosa Equina  aEIAV (Equine infectious anemia virus)1 aNOGUEIRA, M. F.1 aARAÚJO Jr. J. P.