| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
29/11/2021 |
|
Data da última atualização: |
29/11/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
RODRIGUES, C. I.; COSTA, D. M. da; SANTOS, A. C. V.; BATATINHA, M. J. M.; SOUZA, F. V. D.; SOUZA, E. H. de; BOTURA, M. B.; ALVES, C. Q.; SOARES, T. L.; BRANDÃO, H. N. |
|
Afiliação: |
CAROLINE ISSLER RODRIGUES, UEFS; DIEGO MOTA DA COSTA, UEFS; ACIDÁLIA CARINE VIEIRA SANTOS, UFBA; MARIA JOSÉ MOREIRA BATATINHA, UFBA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA, UFRB; MARIANA BORGES BOTURA, UEFS; CLAYTON QUEIROZ ALVES, UEFS; TALIANE LEILA SOARES, UEFS; HUGO NEVES BRANDÃO, UEFS. |
|
Título: |
Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Veterinary Parasitology, v.285, 2020. |
|
ISSN: |
1873-2550 |
|
DOI: |
10.1016/j.vetpar.2020.109219 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Species of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive. MenosSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to i... Mostrar Tudo |
|
Thesagro: |
Abacaxi. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02931naa a2200265 a 4500
001 2136781
005 2021-11-29
008 2020 bl uuuu u00u1 u #d
022 $a1873-2550
024 7 $a10.1016/j.vetpar.2020.109219$2DOI
100 1 $aRODRIGUES, C. I.
245 $aAssessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts.$h[electronic resource]
260 $c2020
520 $aSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive.
650 $aAbacaxi
700 1 $aCOSTA, D. M. da
700 1 $aSANTOS, A. C. V.
700 1 $aBATATINHA, M. J. M.
700 1 $aSOUZA, F. V. D.
700 1 $aSOUZA, E. H. de
700 1 $aBOTURA, M. B.
700 1 $aALVES, C. Q.
700 1 $aSOARES, T. L.
700 1 $aBRANDÃO, H. N.
773 $tVeterinary Parasitology$gv.285, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
|
Data corrente: |
11/08/2014 |
|
Data da última atualização: |
25/05/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
RAPOSO, R. S.; SOUZA, I. G. B.; VELOSO, M. E. da C.; KOBAYASHI, A. K.; LAVIOLA, B. G.; DINIZ, F. M. |
|
Afiliação: |
R.S. Raposo, Universidade Federal do Ceara, Núcleo de Biologia Experimental.; I.G.B. Souza, Universidade Federal do Piauí.; MARCOS EMANUEL DA COSTA VELOSO, CPAMN; ADILSON KENJI KOBAYASHI, CNPAE; BRUNO GALVEAS LAVIOLA, CNPAE; FABIO MENDONCA DINIZ, CPAMN. |
|
Título: |
Development of novel simple sequence repeat markers from a genomic sequence survey database and their application for diversity assessment in Jatropha curcas germplasm from Guatemala. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 3, p. 6099-6106, Aug. 2014. |
|
DOI: |
http://dx.doi.org/10.4238/2014.August.7.25 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The last few years have seen a significant increase in the number of large-scale sequencing projects generating whole genome databases. These sequence databases can be surveyed (genome sequence survey) for tandem repeats as an alternative means to develop microsatellites for monitoring and selecting natural populations and cultivars of Jatropha curcas. A total of 100 tandem repeats were revealed from mining 368 genomic surveyed sequences available in the Kazusa DNA Research Institute database. Twenty microsatellite sequences were successfully amplified, resulting in repeatable and scorable polymerase chain reaction products. Genotyping of J. curcas accessions from the Guatemalan population revealed 18 polymorphic loci. The average number of alleles per locus was 6.9, and allelic sizes ranged from 94 to 299 bp. Expected and observed heterozygosities ranged from 0.118 to 0.906 and from 0.082 to 0.794, respectively. Polymorphic information content values ranged from 0.114 (JcSSR-34) to 0.886 (JcSSR-33) with an average of 0.627. Analysis with Micro-Checker indicated few null alleles for locus JcSSR-37 in Guatemalan populations, which may be a possible cause of its deviation from Hardy-Weinberg equilibrium, even after Bonferroni?s correction. No loci showed significant linkage disequilibrium. These microsatellite loci are expected to be valuable molecular markers in J. curcas because they show high levels of polymorphism and heterozygosity. |
|
Palavras-Chave: |
Biofuel; Energy crop; Genetic diversity; Microsatellites; Microssatélite; Physic nut. |
|
Thesagro: |
Biocombustível; Cultura energética; Euphorbiaceae; Pinhão de purga; Planta oleaginosa. |
|
Categoria do assunto: |
--
G Melhoramento Genético |
|
URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1017730/1/ArtigoMarcosFabioGMR2014.pdf
|
|
Marc: |
LEADER 02543naa a2200325 a 4500
001 2017730
005 2022-05-25
008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2014.August.7.25$2DOI
100 1 $aRAPOSO, R. S.
245 $aDevelopment of novel simple sequence repeat markers from a genomic sequence survey database and their application for diversity assessment in Jatropha curcas germplasm from Guatemala.$h[electronic resource]
260 $c2014
520 $aThe last few years have seen a significant increase in the number of large-scale sequencing projects generating whole genome databases. These sequence databases can be surveyed (genome sequence survey) for tandem repeats as an alternative means to develop microsatellites for monitoring and selecting natural populations and cultivars of Jatropha curcas. A total of 100 tandem repeats were revealed from mining 368 genomic surveyed sequences available in the Kazusa DNA Research Institute database. Twenty microsatellite sequences were successfully amplified, resulting in repeatable and scorable polymerase chain reaction products. Genotyping of J. curcas accessions from the Guatemalan population revealed 18 polymorphic loci. The average number of alleles per locus was 6.9, and allelic sizes ranged from 94 to 299 bp. Expected and observed heterozygosities ranged from 0.118 to 0.906 and from 0.082 to 0.794, respectively. Polymorphic information content values ranged from 0.114 (JcSSR-34) to 0.886 (JcSSR-33) with an average of 0.627. Analysis with Micro-Checker indicated few null alleles for locus JcSSR-37 in Guatemalan populations, which may be a possible cause of its deviation from Hardy-Weinberg equilibrium, even after Bonferroni?s correction. No loci showed significant linkage disequilibrium. These microsatellite loci are expected to be valuable molecular markers in J. curcas because they show high levels of polymorphism and heterozygosity.
650 $aBiocombustível
650 $aCultura energética
650 $aEuphorbiaceae
650 $aPinhão de purga
650 $aPlanta oleaginosa
653 $aBiofuel
653 $aEnergy crop
653 $aGenetic diversity
653 $aMicrosatellites
653 $aMicrossatélite
653 $aPhysic nut
700 1 $aSOUZA, I. G. B.
700 1 $aVELOSO, M. E. da C.
700 1 $aKOBAYASHI, A. K.
700 1 $aLAVIOLA, B. G.
700 1 $aDINIZ, F. M.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 13, n. 3, p. 6099-6106, Aug. 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
