02543naa a2200325 a 450000100080000000500110000800800410001902400520006010000180011224502110013026000090034152014640035065000200181465000240183465000180185865000210187665000220189765300120191965300160193165300220194765300200196965300200198965300150200970000200202470000240204470000210206870000190208970000170210877300920212520177302022-05-25       2014    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.4238/2014.August.7.252DOI1 aRAPOSO, R. S.  aDevelopment of novel simple sequence repeat markers from a genomic sequence survey database and their application for diversity assessment in Jatropha curcas germplasm from Guatemala.h[electronic resource]  c2014  aThe last few years have seen a significant increase in the number of large-scale sequencing projects generating whole genome databases. These sequence databases can be surveyed (genome sequence survey) for tandem repeats as an alternative means to develop microsatellites for monitoring and selecting natural populations and cultivars of Jatropha curcas. A total of 100 tandem repeats were revealed from mining 368 genomic surveyed sequences available in the Kazusa DNA Research Institute database. Twenty microsatellite sequences were successfully amplified, resulting in repeatable and scorable polymerase chain reaction products. Genotyping of J. curcas accessions from the Guatemalan population revealed 18 polymorphic loci. The average number of alleles per locus was 6.9, and allelic sizes ranged from 94 to 299 bp. Expected and observed heterozygosities ranged from 0.118 to 0.906 and from 0.082 to 0.794, respectively. Polymorphic information content values ranged from 0.114 (JcSSR-34) to 0.886 (JcSSR-33) with an average of 0.627. Analysis with Micro-Checker indicated few null alleles for locus JcSSR-37 in Guatemalan populations, which may be a possible cause of its deviation from Hardy-Weinberg equilibrium, even after Bonferroni?s correction. No loci showed significant linkage disequilibrium. These microsatellite loci are expected to be valuable molecular markers in J. curcas because they show high levels of polymorphism and heterozygosity.  aBiocombustível  aCultura energética  aEuphorbiaceae  aPinhão de purga  aPlanta oleaginosa  aBiofuel  aEnergy crop  aGenetic diversity  aMicrosatellites  aMicrossatélite  aPhysic nut1 aSOUZA, I. G. B.1 aVELOSO, M. E. da C.1 aKOBAYASHI, A. K.1 aLAVIOLA, B. G.1 aDINIZ, F. M.  tGenetics and Molecular Research, Ribeirão Pretogv. 13, n. 3, p. 6099-6106, Aug. 2014.