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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
03/10/2008 |
Data da última atualização: |
26/06/2023 |
Autoria: |
VANDER SCHALIE, J.; BRADWAY, D. S.; BESSER, T. S.; EVERMANN, J. F. |
Título: |
Evaluation of a kinetic enzyme-linked immunosorbent assay for detection of caprine arthritis-encephalitis virus-specific antibodies. |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
Journal of Veterinary Diagnostic Investigation, v. 6, n. 1, p. 30-33, Jan. 1994. |
Idioma: |
Inglês |
Conteúdo: |
A kinetic indirect enzyme-linked immunosorbent assay (k-ELISA) was evaluated for detection of antibody to caprine arthritis-encephalitis virus (CAEV), using sodium dodecyl sulfate-treated CAEV-63 as antigen. Two hundred fifteen caprine sera submitted to the diagnostic laboratory were tested for CAEV antibody by the k-ELISA and by mmunoprecipitation of [35S]-methionine-labeled CAEV. A k-ELISA positive cutoff point of 80 yielded a sensitivity of 94.4% and a specificity of 100%, as compared with immunoprecipitation. A k-ELISA cutoff point of 50 resulted in a sensitivity of 100%, with 95.6% specificity. When sera with k-ELISA scores between 50 and 80 were considered suspect, testing of 1,001 diagnostic sera resulted in < 1.5% suspect reactions. Using the 80 cutoff point, the CAEV k-ELISA had good sensitivity and specificity, with the added advantages of quick turn-around time, few suspect reactions, and adaptability to large numbers of samples. |
Palavras-Chave: |
Artrite Encefalite; CAEV; Imunodiagnóstico; Lentivirus Infections; Sulfur Radioisotopes. |
Thesagro: |
Caprino; Doença Animal. |
Thesaurus Nal: |
Antibody specificity; Caprine arthritis-encephalitis; Disease diagnosis; Enzyme-linked immunosorbent assay; Goat diseases; Immunology; Kinetics; Methionine; Precipitin tests; Taste sensitivity; Viral antibodies. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02110naa a2200373 a 4500 001 1516073 005 2023-06-26 008 1994 bl uuuu u00u1 u #d 100 1 $aVANDER SCHALIE, J. 245 $aEvaluation of a kinetic enzyme-linked immunosorbent assay for detection of caprine arthritis-encephalitis virus-specific antibodies.$h[electronic resource] 260 $c1994 520 $aA kinetic indirect enzyme-linked immunosorbent assay (k-ELISA) was evaluated for detection of antibody to caprine arthritis-encephalitis virus (CAEV), using sodium dodecyl sulfate-treated CAEV-63 as antigen. Two hundred fifteen caprine sera submitted to the diagnostic laboratory were tested for CAEV antibody by the k-ELISA and by mmunoprecipitation of [35S]-methionine-labeled CAEV. A k-ELISA positive cutoff point of 80 yielded a sensitivity of 94.4% and a specificity of 100%, as compared with immunoprecipitation. A k-ELISA cutoff point of 50 resulted in a sensitivity of 100%, with 95.6% specificity. When sera with k-ELISA scores between 50 and 80 were considered suspect, testing of 1,001 diagnostic sera resulted in < 1.5% suspect reactions. Using the 80 cutoff point, the CAEV k-ELISA had good sensitivity and specificity, with the added advantages of quick turn-around time, few suspect reactions, and adaptability to large numbers of samples. 650 $aAntibody specificity 650 $aCaprine arthritis-encephalitis 650 $aDisease diagnosis 650 $aEnzyme-linked immunosorbent assay 650 $aGoat diseases 650 $aImmunology 650 $aKinetics 650 $aMethionine 650 $aPrecipitin tests 650 $aTaste sensitivity 650 $aViral antibodies 650 $aCaprino 650 $aDoença Animal 653 $aArtrite Encefalite 653 $aCAEV 653 $aImunodiagnóstico 653 $aLentivirus Infections 653 $aSulfur Radioisotopes 700 1 $aBRADWAY, D. S. 700 1 $aBESSER, T. S. 700 1 $aEVERMANN, J. F. 773 $tJournal of Veterinary Diagnostic Investigation$gv. 6, n. 1, p. 30-33, Jan. 1994.
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6. |  | DIECKOW, J.; BAYER, C.; CONCEIÇÃO, P. C.; ZANATTA, J. A.; MARTIN NETO, L.; MILORI, D. M. B. P.; SALTON, J. C.; MACEDO, M. M.; MIELNICZUK, J.; HERNANI, L. C. Land use, tillage, texture and organic matter stock and composition in tropical and subtropical brazilian soils. European journal of soil science, Oxford, v. 60, p. 240-249, 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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