02110naa a2200373 a 450000100080000000500110000800800410001910000230006024501600008326000090024352009610025265000250121365000350123865000220127365000380129565000180133365000150135165000130136665000150137965000210139465000220141565000210143765000120145865000190147065300230148965300090151265300220152165300260154365300250156970000190159470000180161370000200163177300850165115160732023-06-26       1994    bl uuuu     u00u1 u    #d1 aVANDER SCHALIE, J.  aEvaluation of a kinetic enzyme-linked immunosorbent assay for detection of caprine arthritis-encephalitis virus-specific antibodies.h[electronic resource]  c1994  aA kinetic indirect enzyme-linked immunosorbent assay (k-ELISA) was evaluated for detection of antibody to caprine arthritis-encephalitis virus (CAEV), using sodium dodecyl sulfate-treated CAEV-63 as antigen. Two hundred fifteen caprine sera submitted to the diagnostic laboratory were tested for CAEV antibody by the k-ELISA and by mmunoprecipitation of [35S]-methionine-labeled CAEV. A k-ELISA positive cutoff point of 80 yielded a sensitivity of 94.4% and a specificity of 100%, as compared with immunoprecipitation. A k-ELISA cutoff point of 50 resulted in a sensitivity of 100%, with 95.6% specificity. When sera with k-ELISA scores between 50 and 80 were considered suspect, testing of 1,001 diagnostic sera resulted in &lt 1.5% suspect reactions. Using the 80 cutoff point, the CAEV k-ELISA had good sensitivity and specificity, with the added advantages of quick turn-around time, few suspect reactions, and adaptability to large numbers of samples.  aAntibody specificity  aCaprine arthritis-encephalitis  aDisease diagnosis  aEnzyme-linked immunosorbent assay  aGoat diseases  aImmunology  aKinetics  aMethionine  aPrecipitin tests  aTaste sensitivity  aViral antibodies  aCaprino  aDoença Animal  aArtrite Encefalite  aCAEV  aImunodiagnóstico  aLentivirus Infections  aSulfur Radioisotopes1 aBRADWAY, D. S.1 aBESSER, T. S.1 aEVERMANN, J. F.  tJournal of Veterinary Diagnostic Investigationgv. 6, n. 1, p. 30-33, Jan. 1994.