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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
21/08/1995 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
DURAN-VILA, N.; ORTEGA, V.; NAVARRO, L. |
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Afiliação: |
Instituto Valenciano de Investigacones Agrarias, Apartado Oficial, 46113 Moncada (Valencia), Spain. |
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Título: |
Morphogenesis and tissue cultures of three citrus species. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Plant Cell. Tissue and Organ Culture, v.16, p.123-133, 1989. |
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Idioma: |
Inglês |
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Conteúdo: |
Studies were performed to define tissue culture techniques and culture conditions for morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis (L.) Obs.), citron (C. medica L.) and lime (C. aurantifolia (Christm. Swing). The optimal concentrations of NAA to induce root formation on stem segments were 10 mg l-1 for sweet orange and lime, and 3 mg l-1 for citron. The optimal BA concentration for shoot and bud proliferation was 3 mg l-1 for sweet orange and citron, orange and citron, and l mg l-1 for lime. Callus initiation was accomplished in a culture medium containing 10 mg l-1 NAA and 0.25 mgl-1 BA. Callus was maintained by periodical subculture into the same medium supplemented with 10% (v:v) orange juice. In vitro plantlets of the theree species were obtained by rooting of shoots developed from bud cultures, and of citron and lime by development of shoots from root cultures. The plants were seccessfully established on soil. |
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Palavras-Chave: |
Root culturae. |
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Thesagro: |
Citrus Aurantifolia; Citrus Medica; Citrus Sinensis. |
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Thesaurus Nal: |
callus; morphogenesis; organogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01599naa a2200229 a 4500
001 1646801
005 2023-05-17
008 1989 bl uuuu u00u1 u #d
100 1 $aDURAN-VILA, N.
245 $aMorphogenesis and tissue cultures of three citrus species.$h[electronic resource]
260 $c1989
520 $aStudies were performed to define tissue culture techniques and culture conditions for morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis (L.) Obs.), citron (C. medica L.) and lime (C. aurantifolia (Christm. Swing). The optimal concentrations of NAA to induce root formation on stem segments were 10 mg l-1 for sweet orange and lime, and 3 mg l-1 for citron. The optimal BA concentration for shoot and bud proliferation was 3 mg l-1 for sweet orange and citron, orange and citron, and l mg l-1 for lime. Callus initiation was accomplished in a culture medium containing 10 mg l-1 NAA and 0.25 mgl-1 BA. Callus was maintained by periodical subculture into the same medium supplemented with 10% (v:v) orange juice. In vitro plantlets of the theree species were obtained by rooting of shoots developed from bud cultures, and of citron and lime by development of shoots from root cultures. The plants were seccessfully established on soil.
650 $acallus
650 $amorphogenesis
650 $aorganogenesis
650 $aCitrus Aurantifolia
650 $aCitrus Medica
650 $aCitrus Sinensis
653 $aRoot culturae
700 1 $aORTEGA, V.
700 1 $aNAVARRO, L.
773 $tPlant Cell. Tissue and Organ Culture$gv.16, p.123-133, 1989.
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