01599naa a2200229 a 450000100080000000500110000800800410001910000190006024500860007926000090016552009720017465000110114665000180115765000180117565000240119365000180121765000200123565300180125570000150127370000160128877300650130416468012023-05-17       1989    bl uuuu     u00u1 u    #d1 aDURAN-VILA, N.  aMorphogenesis and tissue cultures of three citrus species.h[electronic resource]  c1989  aStudies were performed to define tissue culture techniques and culture conditions for morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis (L.) Obs.), citron (C. medica L.) and lime (C. aurantifolia (Christm. Swing). The optimal concentrations of NAA to induce root formation on stem segments were 10 mg l-1 for sweet orange and lime, and 3 mg l-1 for citron. The optimal BA concentration for shoot and bud proliferation was 3 mg l-1 for sweet orange and citron, orange and citron, and l mg l-1 for lime. Callus initiation was accomplished in a culture medium containing 10 mg l-1 NAA and 0.25 mgl-1 BA. Callus was maintained by periodical subculture into the same medium supplemented with 10% (v:v) orange juice. In vitro plantlets of the theree species were obtained by rooting of shoots developed from bud cultures, and of citron and lime by development of shoots from root cultures. The plants were seccessfully established on soil.  acallus  amorphogenesis  aorganogenesis  aCitrus Aurantifolia  aCitrus Medica  aCitrus Sinensis  aRoot culturae1 aORTEGA, V.1 aNAVARRO, L.  tPlant Cell. Tissue and Organ Culturegv.16, p.123-133, 1989.