| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
03/05/2013 |
|
Data da última atualização: |
17/01/2024 |
|
Autoria: |
AVELAR, S. R. G.; MOURA, R. R.; SOUSA, F. C.; PEREIRA, A. F.; ALMEIDA, K. C.; MELO, C. H. S.; TELES-FILHO, A. C. A.; BARIL, G.; MELO, L. M.; TEIXEIRA, D. I. A.; FREITAS, V. J. F. |
|
Título: |
Oocyte production and in vitro maturation in Canindé goats following hormonal ovarian stimulation. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Animal Reproduction, v. 9, n. 1, p. 27-32, Jan./Mar. 2012. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: This study evaluated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus-oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro maturation (IVM). Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg medroxyprogesterone acetate for 11 days, together with an injection of 50 ?g d-cloprostenol on the 8th day, along with additional treatment regimens, as follows: i) five doses (5D), 120 mg of NIH-FSH-P1 in five injections at 12 h intervals; ii) three doses (3D), 120 mg of NIH-FSH-P1 in three injections at 24 h intervals; iii) single dose (1D), 70 mg of NIH-FSH-P1 and 200 IU of eCG at 36 h prior to sponge removal. Three sessions of hormonal treatment/oocyte recovery were performed and goats (n = 6/group) were allocated to different groups. The oocytes were collected by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being recovered, resulting in a recovery rate of 74.7% (577/772). The mean number of COCs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). Recovery rate was higher (P < 0.05) in the 5D group (84.1%; 211/251) compared to the 3D (68.2%; 182/267) and 1D groups (72.4%; 184/254). The lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1%; 27/84), while the rate for the 5D and 1D groups was 49.1% (53/108) and 46.2% (42/91), respectively. Finally, taking into account the main performance results of the three treatments, it is advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes. MenosAbstract: This study evaluated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus-oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro maturation (IVM). Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg medroxyprogesterone acetate for 11 days, together with an injection of 50 ?g d-cloprostenol on the 8th day, along with additional treatment regimens, as follows: i) five doses (5D), 120 mg of NIH-FSH-P1 in five injections at 12 h intervals; ii) three doses (3D), 120 mg of NIH-FSH-P1 in three injections at 24 h intervals; iii) single dose (1D), 70 mg of NIH-FSH-P1 and 200 IU of eCG at 36 h prior to sponge removal. Three sessions of hormonal treatment/oocyte recovery were performed and goats (n = 6/group) were allocated to different groups. The oocytes were collected by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being recovered, resulting in a recovery rate of 74.7% (577/772). The mean number of COCs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). Recovery rate was higher (P < 0.05) in the 5D group (84.1%; 211/251) compared to the 3D (68.2%; 182/267) and 1D groups (72.4%; 184/254). The lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1%; 27/84), while the rate for the 5D an... Mostrar Tudo |
|
Palavras-Chave: |
In vitro; Oócito; Raça Canindé. |
|
Thesagro: |
Caprino; Gonadotrofina; Hormônio; Maturação; Ovário; Reprodução animal. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02771naa a2200349 a 4500 001 1957082 005 2024-01-17 008 2012 bl uuuu u00u1 u #d 100 1 $aAVELAR, S. R. G. 245 $aOocyte production and in vitro maturation in Canindé goats following hormonal ovarian stimulation.$h[electronic resource] 260 $c2012 520 $aAbstract: This study evaluated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus-oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro maturation (IVM). Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg medroxyprogesterone acetate for 11 days, together with an injection of 50 ?g d-cloprostenol on the 8th day, along with additional treatment regimens, as follows: i) five doses (5D), 120 mg of NIH-FSH-P1 in five injections at 12 h intervals; ii) three doses (3D), 120 mg of NIH-FSH-P1 in three injections at 24 h intervals; iii) single dose (1D), 70 mg of NIH-FSH-P1 and 200 IU of eCG at 36 h prior to sponge removal. Three sessions of hormonal treatment/oocyte recovery were performed and goats (n = 6/group) were allocated to different groups. The oocytes were collected by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being recovered, resulting in a recovery rate of 74.7% (577/772). The mean number of COCs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). Recovery rate was higher (P < 0.05) in the 5D group (84.1%; 211/251) compared to the 3D (68.2%; 182/267) and 1D groups (72.4%; 184/254). The lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1%; 27/84), while the rate for the 5D and 1D groups was 49.1% (53/108) and 46.2% (42/91), respectively. Finally, taking into account the main performance results of the three treatments, it is advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes. 650 $aCaprino 650 $aGonadotrofina 650 $aHormônio 650 $aMaturação 650 $aOvário 650 $aReprodução animal 653 $aIn vitro 653 $aOócito 653 $aRaça Canindé 700 1 $aMOURA, R. R. 700 1 $aSOUSA, F. C. 700 1 $aPEREIRA, A. F. 700 1 $aALMEIDA, K. C. 700 1 $aMELO, C. H. S. 700 1 $aTELES-FILHO, A. C. A. 700 1 $aBARIL, G. 700 1 $aMELO, L. M. 700 1 $aTEIXEIRA, D. I. A. 700 1 $aFREITAS, V. J. F. 773 $tAnimal Reproduction$gv. 9, n. 1, p. 27-32, Jan./Mar. 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 6 | |
| 2. |  | FERRARI, J. V.; FURLANI JÚNIOR, E.; FERRARI, S.; SANTOS, D. M. A. dos; FERNANDES, A. R.; MARTINS, L. E. C. Produtividade e crescimento do algodoeiro em função de espaçamentos e aplicação de regulador de crescimento In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-6 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
|   |
| 3. |  | MARTINS, L. E. C.; FURLANI JÚNIOR, E.; FERRARI, J. V.; FERRARI, S.; FERNANDES, A. R.; VAL, H. C. do. Plantio direto e aplicação de Nitrogênio em pré-semeadura sobre o desenbolbimento vegetativo do algodoeiro. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-4 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
|   |
| 4. |  | SANTOS, D. M. A. dos; FURLANI JUNIOR, E.; FERRARI, S.; ALPE, V.; FERRARI, J. V.; MARTINS, L. E. C.; MADEIRA, T. A. Avaliação das características morfológicas do algodoeiro em relação ao modo de aplicação e doses crescentes de Nitrogênio. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-4 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
|   |
| 5. |  | ALPE, V.; FURLANI JUNIOR, E.; SANTOS, D. M. A. dos; FERRARI, S.; SANTOS, M. L. dos; MARTINS, L. E. C.; FERRARI, J. V.; VAL, H. C. do. Estado nutricional da cultura do algodoeiro através de doses de Nitrogênio aplicado de modo único e parcelado. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-6 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
|   |
| 6. |  | SANTOS, D. M. A. dos; FURLANI JUNIOR, E.; FERRARI, S.; ALPE, V.; FERRARI, J. V.; MARTINS, L. E. C.; SANTOS, M. L. dos. Estado nutricional da planta de algodão através da aplicação de doses crescentes de N em diferentes modos de aplicação. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Anais... Uberlândia, 2007. p. 1-5 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
|   |
| Registros recuperados : 6 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|