02771naa a2200349 a 450000100080000000500110000800800410001910000210006024501270008126000090020852018090021765000120202665000180203865000140205665000160207065000120208665000240209865300130212265300120213565300190214770000170216670000170218370000190220070000190221970000190223870000260225770000140228370000160229770000230231370000220233677300630235819570822024-01-17       2012    bl uuuu     u00u1 u    #d1 aAVELAR, S. R. G.  aOocyte production and in vitro maturation in Canindé goats following hormonal ovarian stimulation.h[electronic resource]  c2012  aAbstract: This study evaluated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus-oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro maturation (IVM). Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg medroxyprogesterone acetate for 11 days, together with an injection of 50 ?g d-cloprostenol on the 8th day, along with additional treatment regimens, as follows: i) five doses (5D), 120 mg of NIH-FSH-P1 in five injections at 12 h intervals; ii) three doses (3D), 120 mg of NIH-FSH-P1 in three injections at 24 h intervals; iii) single dose (1D), 70 mg of NIH-FSH-P1 and 200 IU of eCG at 36 h prior to sponge removal. Three sessions of hormonal treatment/oocyte recovery were performed and goats (n = 6/group) were allocated to different groups. The oocytes were collected by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being recovered, resulting in a recovery rate of 74.7% (577/772). The mean number of COCs/goat did not differ (P &gt 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). Recovery rate was higher (P &lt 0.05) in the 5D group (84.1%; 211/251) compared to the 3D (68.2%; 182/267) and 1D groups (72.4%; 184/254). The lowest (P &lt 0.05) maturation rate was recorded in the 3D group (32.1%; 27/84), while the rate for the 5D and 1D groups was 49.1% (53/108) and 46.2% (42/91), respectively. Finally, taking into account the main performance results of the three treatments, it is advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes.  aCaprino  aGonadotrofina  aHormônio  aMaturação  aOvário  aReprodução animal  aIn vitro  aOócito  aRaça Canindé1 aMOURA, R. R.1 aSOUSA, F. C.1 aPEREIRA, A. F.1 aALMEIDA, K. C.1 aMELO, C. H. S.1 aTELES-FILHO, A. C. A.1 aBARIL, G.1 aMELO, L. M.1 aTEIXEIRA, D. I. A.1 aFREITAS, V. J. F.  tAnimal Reproductiongv. 9, n. 1, p. 27-32, Jan./Mar. 2012.