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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
27/04/2010 |
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Data da última atualização: |
12/06/2025 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
STEINBERG, R. S.; PEREIRA, L.; LACORTE, G. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; TEODORO, R. L.; MACHADO, M. A.; FONSECA, C. G.; CARVALHO, M. R. S. |
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Afiliação: |
UNIVERSIDADE FEDERAL DE MINAS GERAIS; UNIVERSIDADE FEDERAL DE MINAS GERAIS; UNIVERSIDADE FEDERAL DE MINAS GERAIS; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; UNIVERSIDADE FEDERAL DE MINAS GERAIS; UNIVERSIDADE FEDERAL DE JUIZ DE FORA. |
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Título: |
A new and cost-effective method for detection of the bovine acyl-CoA:diacylglycerol acyltransferase 1 K232A polymorphism in cattle. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 92, p. 773-776, 2009. |
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Volume: |
92 |
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Idioma: |
Inglês |
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Notas: |
Nota Técnica. |
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Conteúdo: |
A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferasel (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT! gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGATI K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGATI genotyping for gene-assisted selection. |
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Palavras-Chave: |
Gado de leite. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/711912/1/A-new-and-cost-effective-method-for-detection.pdf
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Marc: |
LEADER 01731naa a2200265 a 4500
001 1711912
005 2025-06-12
008 2009 bl uuuu u00u1 u #d
100 1 $aSTEINBERG, R. S.
245 $aA new and cost-effective method for detection of the bovine acyl-CoA$bdiacylglycerol acyltransferase 1 K232A polymorphism in cattle.$h[electronic resource]
260 $c2009
300 $a92
490 $v92
500 $aNota Técnica.
520 $aA new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferasel (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT! gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGATI K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGATI genotyping for gene-assisted selection.
653 $aGado de leite
700 1 $aPEREIRA, L.
700 1 $aLACORTE, G. A.
700 1 $aPEIXOTO, M. G. C. D.
700 1 $aVERNEQUE, R. da S.
700 1 $aTEODORO, R. L.
700 1 $aMACHADO, M. A.
700 1 $aFONSECA, C. G.
700 1 $aCARVALHO, M. R. S.
773 $tJournal of Dairy Science$gv. 92, p. 773-776, 2009.
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Embrapa Gado de Leite (CNPGL) |
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