01731naa a2200265 a 450000100080000000500110000800800410001910000210006024501600008126000090024130000070025049000070025750000190026452009460028365300180122970000160124770000190126370000250128270000230130770000190133070000190134970000190136870000230138777300550141017119122025-06-12       2009    bl uuuu     u00u1 u    #d1 aSTEINBERG, R. S.  aA new and cost-effective method for detection of the bovine acyl-CoAbdiacylglycerol acyltransferase 1 K232A polymorphism in cattle.h[electronic resource]  c2009  a92  v92  aNota Técnica.  aA new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferasel (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT! gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGATI K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGATI genotyping for gene-assisted selection.  aGado de leite1 aPEREIRA, L.1 aLACORTE, G. A.1 aPEIXOTO, M. G. C. D.1 aVERNEQUE, R. da S.1 aTEODORO, R. L.1 aMACHADO, M. A.1 aFONSECA, C. G.1 aCARVALHO, M. R. S.  tJournal of Dairy Sciencegv. 92, p. 773-776, 2009.