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| Registros recuperados : 93 | |
| 5. |  | VESCHI, J. L. A.; MARTINS, R. J.; ZAFALON, L. F.; RAMOS, E. M.; CASTRO, R. S. Soroprevalência da CAE em caprinos leiteiros do território do sisal, Bahia. Biológico, v. 73, (Supl. 2), p. 25, 2011.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | VESCHI, J. L. A.; LANDIM, A. M. de S.; RAMOS, E. M.; ZAFALON, L. F. Levantamento de brucelose em ovinos e caprinos da região do submédio do Vale do São Francisco, Pernambuco, Brasil. Acta Scientiae Veterinariae, v. 40 p. s98, 2012. Suplemento 2. Edição do 1 Encontro Nacional de Epidemiologia Veterinária - ENEPI, São Paulo, jul. 2012.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| 19. | | AMORIM, V. da S.; VESCHI, J. L. A.; SILVA, I. F. da; ROSA, D. S.; COSTA, M. M. da. Sensibilidade antimicrobiana de Staphylococcus spp. isolados de bovinos, bubalinos e caprinos com mastite. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 11., 2016, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2016. p. 117-122. (Embrapa Semiárido. Documentos, 271).| Biblioteca(s): Embrapa Semiárido. |
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| 20. | | VESCHI, J. L. A.; LANDIM, A. M. de S.; RAMOS, E. M.; ZAFALON, L. F.; CASTRO, R. S. de. Soroprevalência de lentiviroses de pequenos ruminantes em ovinos e caprinos na região do submédio do Vale do São Francisco, Pernambuco, Brasil. Acta Scientiae Veterinariae, v. 40, p. s99, 2012. Suplemento 2. Edição do 1 Encontro Nacional de Epidemiologia Veterinária - ENEPI, São Paulo, jul. 2012.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| Registros recuperados : 93 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
12/06/2023 |
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Data da última atualização: |
12/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
RAMOS, C. P.; DORNELES, E. M. S.; HAAS, D. J.; VESCHI, J. L. A.; LOUREIRO, D.; PORTELA, R. D.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. |
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Afiliação: |
CAROLINA PANTUZZA RAMOS, UFMG; ELAINE MARIA SELES DORNELES, UFLA; DIONEI JOAQUIM HAAS, UFMG; JOSIR LAINE APARECIDA VESCHI, CPATSA; DAN LOUREIRO, UFBA; RICARDO DIAS PORTELA, UFBA; VASCO AZEVEDO, UFMG; MARCOS BRYAN HEINEMANN, USP - São Paulo; ANDREY PEREIRA LAGE, UFMG. |
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Título: |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Ciência Rural, v. 52, n. 11, e20210328, 2022. |
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DOI: |
http://doi.org/10.1590/0103-8478cr20210328 |
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Idioma: |
Inglês |
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Conteúdo: |
The aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis. MenosThe aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could ... Mostrar Tudo |
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Palavras-Chave: |
Epidemiologia molecular; ERIC 1+2-PCR; Genotipagem. |
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Thesagro: |
Doença Animal; Linfadenite Caseosa. |
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Thesaurus NAL: |
Caseous lymphadenitis; Genotyping; Molecular epidemiology. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154376/1/Molecular-characterization-of-Corynebacterium-pseudotuberculosis-2022.pdf
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Marc: |
LEADER 02705naa a2200325 a 4500
001 2154376
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024 7 $ahttp://doi.org/10.1590/0103-8478cr20210328$2DOI
100 1 $aRAMOS, C. P.
245 $aMolecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR.$h[electronic resource]
260 $c2022
520 $aThe aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis.
650 $aCaseous lymphadenitis
650 $aGenotyping
650 $aMolecular epidemiology
650 $aDoença Animal
650 $aLinfadenite Caseosa
653 $aEpidemiologia molecular
653 $aERIC 1+2-PCR
653 $aGenotipagem
700 1 $aDORNELES, E. M. S.
700 1 $aHAAS, D. J.
700 1 $aVESCHI, J. L. A.
700 1 $aLOUREIRO, D.
700 1 $aPORTELA, R. D.
700 1 $aAZEVEDO, V.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
773 $tCiência Rural$gv. 52, n. 11, e20210328, 2022.
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Embrapa Semiárido (CPATSA) |
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