02705naa a2200325 a 450000100080000000500110000800800410001902400520006010000170011224501390012926000090026852017300027765000260200765000150203365000270204865000190207565000240209465300280211865300170214665300160216370000230217970000160220270000210221870000170223970000190225670000160227570000210229170000160231277300510232821543762023-06-12       2022    bl uuuu     u00u1 u    #d7 ahttp://doi.org/10.1590/0103-8478cr202103282DOI1 aRAMOS, C. P.  aMolecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR.h[electronic resource]  c2022  aThe aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis.  aCaseous lymphadenitis  aGenotyping  aMolecular epidemiology  aDoença Animal  aLinfadenite Caseosa  aEpidemiologia molecular  aERIC 1+2-PCR  aGenotipagem1 aDORNELES, E. M. S.1 aHAAS, D. J.1 aVESCHI, J. L. A.1 aLOUREIRO, D.1 aPORTELA, R. D.1 aAZEVEDO, V.1 aHEINEMANN, M. B.1 aLAGE, A. P.  tCiência Ruralgv. 52, n. 11, e20210328, 2022.