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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/03/2011 |
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Data da última atualização: |
29/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, F. M. V.; MILORI, D. M. B. P.; PEREIRA-FILHO, E. R.; VENANCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTUA, J. |
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Afiliação: |
DEBORA MARCONDES BASTOS P MILORI, CNPDIA. |
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Título: |
Fluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Analytical methods, [S. l.], v. 3, p. 552-556, 2011. |
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DOI: |
10.1039/C0AY00538J |
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Idioma: |
Inglês |
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Conteúdo: |
The citrus greening (or huanglongbing) disease has caused serious problems in citrus crops around the world. An early diagnostic method to detect this malady is needed due to the rapid dissemination of Candidatus Liberibacter asiaticus (CLas) in the field. This analytical study investigated the fluorescence responses of leaves from healthy citrus plants and those inoculated with CLas by images from a stereomicroscope and also evaluated their potential for the early diagnosis of the infection caused by this bacterium. The plants were measured monthly, and the evolution of the bacteria on inoculated plants was monitored by real-time quantitative polymerase chain reaction (RT-qPCR) amplication of CLas sequences. A statistical method was used to analyse the data. The selection of variables from histograms of colours (colourgrams) of the images was optimized using a paired Student 's t-test. The intensity of counts for green colours from images of fluorescence had clearly minor variations for healthy plants than diseased ones. The darker green colours were the indicators of healthy plants and the light colours for the diseased. The method of fluorescence images is novel for fingerprinting healthy and diseased plants and provides an alternative to the current method represented by PCR and visual inspection. A new, non-subjective pattern of analysis and a non-destructive method has been introduced that can minimize the time and costs of analyses. |
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Palavras-Chave: |
Citrus greening disease; Doença do greening citrus; Huanglongbing; Method of fluorescence images; Método de imagens de fluorescência; Plant disease; Statistical method. |
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Thesagro: |
Bactéria; Greening; Método Estatístico. |
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Thesaurus Nal: |
Candidatus Liberibacter. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02525naa a2200337 a 4500
001 1900658
005 2019-01-29
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1039/C0AY00538J$2DOI
100 1 $aPEREIRA, F. M. V.
245 $aFluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection.$h[electronic resource]
260 $c2011
520 $aThe citrus greening (or huanglongbing) disease has caused serious problems in citrus crops around the world. An early diagnostic method to detect this malady is needed due to the rapid dissemination of Candidatus Liberibacter asiaticus (CLas) in the field. This analytical study investigated the fluorescence responses of leaves from healthy citrus plants and those inoculated with CLas by images from a stereomicroscope and also evaluated their potential for the early diagnosis of the infection caused by this bacterium. The plants were measured monthly, and the evolution of the bacteria on inoculated plants was monitored by real-time quantitative polymerase chain reaction (RT-qPCR) amplication of CLas sequences. A statistical method was used to analyse the data. The selection of variables from histograms of colours (colourgrams) of the images was optimized using a paired Student 's t-test. The intensity of counts for green colours from images of fluorescence had clearly minor variations for healthy plants than diseased ones. The darker green colours were the indicators of healthy plants and the light colours for the diseased. The method of fluorescence images is novel for fingerprinting healthy and diseased plants and provides an alternative to the current method represented by PCR and visual inspection. A new, non-subjective pattern of analysis and a non-destructive method has been introduced that can minimize the time and costs of analyses.
650 $aCandidatus Liberibacter
650 $aBactéria
650 $aGreening
650 $aMétodo Estatístico
653 $aCitrus greening disease
653 $aDoença do greening citrus
653 $aHuanglongbing
653 $aMethod of fluorescence images
653 $aMétodo de imagens de fluorescência
653 $aPlant disease
653 $aStatistical method
700 1 $aMILORI, D. M. B. P.
700 1 $aPEREIRA-FILHO, E. R.
700 1 $aVENANCIO, A. L.
700 1 $aRUSSO, M. de S. T.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS-ASTUA, J.
773 $tAnalytical methods, [S. l.]$gv. 3, p. 552-556, 2011.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
06/12/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VARGAS HOYOS, H. A.; SANTOS, S. N.; SILVA, L. J. da; SILVA, F. S. P. da; GENUÁRIO, D. B.; ZUCCHI, T. D.; MELO, I. S. de. |
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Afiliação: |
HAROLD ALEXANDER VARGAS HOYOS; SUIKINAI NOBRE SANTOS; LEONARDO JOSÉ DA SILVA; FÁBIO SÉRGIO PAULINO DA SILVA; DIEGO BONALDO GENUÁRIO; TIAGO DOMINGUES ZUCCHI, Hinove Agrociência; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Streptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, v. 69, n. 8, p. 2431?2439, 2019. |
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ISSN: |
1466-5034 |
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DOI: |
https://doi.org/10.1099/ijsem.0.003498 |
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Idioma: |
Inglês |
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Conteúdo: |
A novel actinobacterium, designated strain 1AS2cT, was isolated from the wheat rhizosphere collected from the Brazilian Cerrado biome. A polyphasic approach, including phenotypic characterization and phylogenetic multilocus sequence analysis (MLSA), was used to determine the taxonomic position of this strain. Analysis of the 16S rRNA gene sequence indicated that the novel strain is closely related to Streptomyces cavourensis NBRC 13026T, Streptomyces albolongus NBRC 13465T and Streptomyces araujoniae ASBV-1T, sharing a similarity value of 98.2, 98.1 and 97.9%, respectively. Additionally, MLSA of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed evolutionary distances beyond the 0.007 threshold, as well as low DNA-DNA relatedness between strain 1AS2cT and its closest phylogenetic neighbours ( S. cavourensis NBRC 13026T, S. albolongus NBRC 13465T and S. araujoniae ASBV-1T: 56, 62.5 and 63.0%, respectively), indicating a new phylogenetic lineage. The phylogenetic, chemotaxonomic and phenotypic characteristics support the assignment of strain 1AS2cT to the genus Streptomyces , representing a novel species. It is concluded that strain 1AS2cT (=CMAA 1679T=NRRL B-65479T=DSM 105299T) can be classified as the type strain of a novel species of the genus Streptomyces , for which the name Streptomyces rhizosphaericola sp. nov. is proposed. |
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Palavras-Chave: |
Polyphasic approaches; Streptomyces rhizosphaericola sp nov. |
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Thesagro: |
Bactéria. |
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Thesaurus NAL: |
Actinobacteria; Rhizosphere bacteria; Wheat. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02295naa a2200289 a 4500
001 2116198
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
022 $a1466-5034
024 7 $ahttps://doi.org/10.1099/ijsem.0.003498$2DOI
100 1 $aVARGAS HOYOS, H. A.
245 $aStreptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere.$h[electronic resource]
260 $c2019
520 $aA novel actinobacterium, designated strain 1AS2cT, was isolated from the wheat rhizosphere collected from the Brazilian Cerrado biome. A polyphasic approach, including phenotypic characterization and phylogenetic multilocus sequence analysis (MLSA), was used to determine the taxonomic position of this strain. Analysis of the 16S rRNA gene sequence indicated that the novel strain is closely related to Streptomyces cavourensis NBRC 13026T, Streptomyces albolongus NBRC 13465T and Streptomyces araujoniae ASBV-1T, sharing a similarity value of 98.2, 98.1 and 97.9%, respectively. Additionally, MLSA of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed evolutionary distances beyond the 0.007 threshold, as well as low DNA-DNA relatedness between strain 1AS2cT and its closest phylogenetic neighbours ( S. cavourensis NBRC 13026T, S. albolongus NBRC 13465T and S. araujoniae ASBV-1T: 56, 62.5 and 63.0%, respectively), indicating a new phylogenetic lineage. The phylogenetic, chemotaxonomic and phenotypic characteristics support the assignment of strain 1AS2cT to the genus Streptomyces , representing a novel species. It is concluded that strain 1AS2cT (=CMAA 1679T=NRRL B-65479T=DSM 105299T) can be classified as the type strain of a novel species of the genus Streptomyces , for which the name Streptomyces rhizosphaericola sp. nov. is proposed.
650 $aActinobacteria
650 $aRhizosphere bacteria
650 $aWheat
650 $aBactéria
653 $aPolyphasic approaches
653 $aStreptomyces rhizosphaericola sp nov
700 1 $aSANTOS, S. N.
700 1 $aSILVA, L. J. da
700 1 $aSILVA, F. S. P. da
700 1 $aGENUÁRIO, D. B.
700 1 $aZUCCHI, T. D.
700 1 $aMELO, I. S. de
773 $tInternational Journal of Systematic and Evolutionary Microbiology$gv. 69, n. 8, p. 2431?2439, 2019.
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Embrapa Meio Ambiente (CNPMA) |
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