02295naa a2200289 a 450000100080000000500110000800800410001902200140006002400480007410000240012224501230014626000090026952013690027865000190164765000250166665000100169165000140170165300260171565300410174170000180178270000200180070000230182070000210184370000180186470000190188277301040190121161982019-12-06       2019    bl uuuu     u00u1 u    #d  a1466-50347 ahttps://doi.org/10.1099/ijsem.0.0034982DOI1 aVARGAS HOYOS, H. A.  aStreptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere.h[electronic resource]  c2019  aA novel actinobacterium, designated strain 1AS2cT, was isolated from the wheat rhizosphere collected from the Brazilian Cerrado biome. A polyphasic approach, including phenotypic characterization and phylogenetic multilocus sequence analysis (MLSA), was used to determine the taxonomic position of this strain. Analysis of the 16S rRNA gene sequence indicated that the novel strain is closely related to Streptomyces cavourensis NBRC 13026T, Streptomyces albolongus NBRC 13465T and Streptomyces araujoniae ASBV-1T, sharing a similarity value of 98.2, 98.1 and 97.9%, respectively. Additionally, MLSA of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed evolutionary distances beyond the 0.007 threshold, as well as low DNA-DNA relatedness between strain 1AS2cT and its closest phylogenetic neighbours ( S. cavourensis NBRC 13026T, S. albolongus NBRC 13465T and S. araujoniae ASBV-1T: 56, 62.5 and 63.0%, respectively), indicating a new phylogenetic lineage. The phylogenetic, chemotaxonomic and phenotypic characteristics support the assignment of strain 1AS2cT to the genus Streptomyces , representing a novel species. It is concluded that strain 1AS2cT (=CMAA 1679T=NRRL B-65479T=DSM 105299T) can be classified as the type strain of a novel species of the genus Streptomyces , for which the name Streptomyces rhizosphaericola sp. nov. is proposed.  aActinobacteria  aRhizosphere bacteria  aWheat  aBactéria  aPolyphasic approaches  aStreptomyces rhizosphaericola sp nov1 aSANTOS, S. N.1 aSILVA, L. J. da1 aSILVA, F. S. P. da1 aGENUÁRIO, D. B.1 aZUCCHI, T. D.1 aMELO, I. S. de  tInternational Journal of Systematic and Evolutionary Microbiologygv. 69, n. 8, p. 2431?2439, 2019.