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| Registros recuperados : 41 | |
| 5. |  | TEIXEIRA, M. F. da S.; LAMBERT, V.; MSELLI-LAKAHL, L.; CHETTAB, A.; CHEBLOUNE, Y.; MORNEX, J. F. Immortalization of caprine fibroblasts permissive for replication of small ruminant lentiviruses. American Journal of Veterinary Research, v.58, n.6, p.579-584, 1997.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 6. | | SILVA, D. F.; PEIXOTO, R. M.; ARAÚJO, J. F.; DAMASCENO, E. M.; TEIXEIRA, M. F. da S.; ANDRIOLI, A. Avaliação comparativa de diluidores na manutenção dos parâmetros seminais de caprinos. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 5., 2016, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2016. p. 29-30. [ALICE ANDRIOLI PINHEIRO].| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | RICARTE, A. R. F.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; BÁO, S. N.; SILVA, J. B. A. da. Análise molecular e ultraestrutural de espermatozóides caprinos oriundos de animais infectados naturalmente e experimentalmente com o CAEV. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010. 4 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | RODRIGUES, A. P. R.; PINTO, M. T.; TEIXEIRA, M. F. da S.; MELO, A. C. M.; FREITAS, V. J. de F. Análise macro e microscópica das lesões em caprinos soropositivos para o vírus da artrite-encefalite caprina sem a presença de sinais clínicos. Ciência Animal, Fortaleza, v. 11, n. 2, p. 113-118, 2001.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | PEIXOTO, R. M.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; SILVA, A. A. de S.; AGUIAR, T. D. de F.; SOUSA, A. L. M. de. Perspectivas do uso de fitoterápicos no tratamento do sêmen caprino infectado pelo vírus da Artrite Encefalite Caprina. Medicina Veterinária (UFRPE), v. 12, n. 3, p. 193-201, jul/set. 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | FERREIRA, R. C. S.; TEIXEIRA, M. F. da S.; ROCHA, M. F. G.; LIMA, S. M. C.; FROTA, M. N. L. da; MELO, A. C. M. Redução da população microbiana presente no leite de cabra submetido à temperatura de 56oC por 60 minutos. Ciência Animal, Fortaleza, v. 11, n. 1, p. 39-44, 2001.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | AZEVEDO, D. A. A. de; SANTOS, V. W. S. dos; SOUSA, A. L. M. de; PEIXOTO, R. M.; PINHEIRO, R. R.; ANDRIOLI, A.; TEIXEIRA, M. F. da S. Small ruminant lentiviruses: economic and productive losses, consequences of the disease. Arquivos do Instituto Biológico, v. 84, p. 1-10, e0552016, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | GÓES, C. D. M. de M. B.; TEIXEIRA, M. F. da S.; ALVES, F. S. F.; BATISTA, L. M.; BEVILAQUA, C. M. L. Avaliação da transferência passiva de anticorpos em cabritos alimentados com três diferentes tipos de colostro. Ciência Animal, Fortaleza, v. 8, n. 2, p. 57-61, 1998.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | SOUZA, K. C. de; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R.; BEZERRA JUNIOR, R. Q.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. Detecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva. Acta Scientiae Veterinariae, v. 43, n. 1266, p. 1-6, 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | FERRAZ, R. E. de O.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; MELO, V. S. P. de; DANTAS, T. V. M.; CAMINHA, C. M. M.; BARROSO, I. C. Lavagem do sêmen de reprodutores caprinos com solução Krebs-Ringer-Fosfato no controle da Artrite Encefalite Caprina (CAE). In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 4.; FEIRA NACIONAL DO AGRONEGÓCIO DA CAPRINO-OVINOCULTURA DE CORTE, 3., 2009, João Pessoa. Anais... João Pessoa: EMEPA-PB, 2009. 3 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | PEIXOTO, R. M.; ANDRIOLI, A.; PINHEIRO, R. R.; ALVES, F. S. F.; SANTOS, V. W. S. dos; SOUSA, M. M. de; AZEVEDO, D. A. A. de; DAMASCENO, E. M.; TEIXEIRA, M. F. da S. Mycoplasma agalactiae em rebanhos leiteiros no estado do Ceará em associação com o vírus da artrite encefalite caprina. Acta Scientiae Veterinariae, v. 46, Pub. 1533, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | AZEVEDO, D. A. A. de; MONTEIRO, J. P.; PINHEIRO, R. R.; MUDADU, M. de A.; ANDRIOLI, A.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes. Small Ruminant Research, v. 177, p. 160-166, 2019.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Caprinos e Ovinos. |
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| 18. | | SIDER, L. H.; VERAS, A. K. A.; OLIVEIRA, A. N. de; BRITO, R. L. L. de; ANDRIOLI, A.; PINHEIRO, R. R.; RAVAZZOLO, A. P.; TEIXEIRA, M. F. da S. Diagnóstico do vírus da artrite-encefalite caprina por RT-Nested PCD. In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. [Resumos...]. Campo Grande, MS: Embrapa Gado de Corte, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | FEITOSA, A. L. V. L.; TEIXEIRA, M. F. da S.; PINHEIRO, R. R.; CUNHA, R. M. S. da; LIMA, J. P. M. S.; ANDRIOLI, A.; DANTAS, T. V. M.; MELO, V. S. P. de; PINHEIRO, D. C. S. N. Phylogenetic analysis of small ruminant lentiviruses from Northern Brazil. Small Ruminant Research, v. 94, n. 1, p. 205-209, Nov., 2010.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | PEIXOTO, R. M.; TEIXEIRA, M. F. da S.; PINHEIRO, A. A.; PINHEIRO, R. R.; SILVA, A. A. de S.; AGUIAR, T. D. de F.; AZEVEDO, D. A. A. de; SOUSA, A. L. M. de. Perspectivas do uso de fitoterápicos no tratamento do sêmen caprino infectado pelo vírus da Artrite Encefalite Caprina. Medicina Veterinária (UFRPE), v. 12, n. 3, p. 193-201, jul./set. 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 41 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
10/12/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; PEIXOTO, R. M.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; LIMA, A. M. C.; NOBRE, J. A.; AMARAL, G. P.; BRANDÃO, I. S.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO, State University of Ceará - Fortaleza, Ceará, Brazil; ALICE ANDRIOLI PINHEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; RENATO MESQUITA PEIXOTO, Scholarship for Regional Scientific Development of the National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Level C, Brasilia, Distrito Federal, Brazil; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO, State University of Ceará - Fortaleza, Ceará, Brazil; ANA MILENA CESAR LIMA, Federal University of Piauí - Teresina, Piauí, Brazil; JULIANA ARAÚJO NOBRE, State University of Ceará - Fortaleza, Ceará, Brazil; GABRIEL PAULA AMARAL, State University of Acaraú Valley, Sobral, Ceará, Brazil; IANE SOUSA BRANDÃO, State University of Acaraú Valley, Sobral, Ceará, Brazil; MARIA FÁTIMA DA SILVA TEIXEIRA, State University of Ceará - Fortaleza, Ceará, Brazil. |
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Título: |
Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Comparative Immunology, Microbiology and Infectious Diseases, v. 78, 101693, Oct. 2021. |
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DOI: |
https://doi.org/10.1016/j.cimid.2021.101693 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission. MenosAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally i... Mostrar Tudo |
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Palavras-Chave: |
Amniotic sac; Intrauterine transmission; Retrovirus; SRLVs; Vertical disease transmission. |
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Thesaurus NAL: |
Body fluids; Disease diagnosis; Goat diseases; Goats; Risk factors. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02839naa a2200373 a 4500
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008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cimid.2021.101693$2DOI
100 1 $aARAÚJO, J. F.
245 $aDetection and isolation of small ruminant lentivirus in the amniotic fluid of goats.$h[electronic resource]
260 $c2021
520 $aAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission.
650 $aBody fluids
650 $aDisease diagnosis
650 $aGoat diseases
650 $aGoats
650 $aRisk factors
653 $aAmniotic sac
653 $aIntrauterine transmission
653 $aRetrovirus
653 $aSRLVs
653 $aVertical disease transmission
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aPEIXOTO, R. M.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aLIMA, A. M. C.
700 1 $aNOBRE, J. A.
700 1 $aAMARAL, G. P.
700 1 $aBRANDÃO, I. S.
700 1 $aTEIXEIRA, M. F. da S.
773 $tComparative Immunology, Microbiology and Infectious Diseases$gv. 78, 101693, Oct. 2021.
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