02839naa a2200373 a 450000100080000000500110000800800410001902400530006010000190011324501120013226000090024452017270025365000160198065000220199665000180201865000100203665000170204665300170206365300300208065300150211065300100212565300340213570000170216970000200218670000190220670000230222570000250224870000190227370000170229270000180230970000200232770000260234777300920237321374802021-12-10       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.cimid.2021.1016932DOI1 aARAÚJO, J. F.  aDetection and isolation of small ruminant lentivirus in the amniotic fluid of goats.h[electronic resource]  c2021  aAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission.  aBody fluids  aDisease diagnosis  aGoat diseases  aGoats  aRisk factors  aAmniotic sac  aIntrauterine transmission  aRetrovirus  aSRLVs  aVertical disease transmission1 aANDRIOLI, A.1 aPINHEIRO, R. R.1 aPEIXOTO, R. M.1 aSOUSA, A. L. M. de1 aAZEVEDO, D. A. A. de1 aLIMA, A. M. C.1 aNOBRE, J. A.1 aAMARAL, G. P.1 aBRANDÃO, I. S.1 aTEIXEIRA, M. F. da S.  tComparative Immunology, Microbiology and Infectious Diseasesgv. 78, 101693, Oct. 2021.