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| Registros recuperados : 2 | |
| 2. |  | MARIANO, A. B.; KOVALHULK, L.; VALENTE, C.; MAURER-MENESTRINA, J.; PEREIRA-NETTO, A. B.; GUERRA, M. P.; CARNIERI, E. G. S. Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze. Brazilian Archives of Biology and Technology: an International Journal, Curitiba, v. 47, n. 6, p. 873-879, Nov. 2004.| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 2 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
19/02/2019 |
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Data da última atualização: |
21/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
SILVA, V. B. da; SILVA, A. F. da; SILVA, T. R. da; SANTOS, J. W. M. dos; SILVA, J. F. da; SOUZA, A. P. de; FREITAS, A. D. S. de; FERNANDES JUNIOR, P. I. |
|
Afiliação: |
VALÉRIA BORGES DA SILVA; ALEKSANDRO FERREIRA DA SILVA; THAISE ROSA DA SILVA; JONNATHAN WHINY MORAES DOS SANTOS; JÉSSICA FERNANDA DA SILVA; ADAILSON PEREIRA DE SOUZA; ANA DOLORES SANTIAGO DE FREITAS; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Fast and efficient symbiotic gene-based duplex PCR approach for the preliminary selection of legume root nodule bacteria. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Rhizosphere, v. 10, 2019. |
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ISSN: |
2452-2198 |
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DOI: |
10.1016/j.rhisph.2019.100144 |
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Idioma: |
Inglês |
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Conteúdo: |
The isolation of root nodule bacteria (RNB) usually lead to obtainment of several non-rhizobia, mainly those fastgrowing bacteria. The plant-authentication experiments to select the nodulating bacteria are time consuming and can be ruined in case of nodulation of negative controls. To speed up this step in rhizobiology research a fast, simple and efficient duplex PCR assay for the amplification of nodC and nifH genes in was developed aiming to separate rhizobia and non-rhizobia in RNB culture collections. The method was optimized with 43 reference strains and was applied to a new culture collection for validation. Considering all bacteria, 106 out of 109 nodulating rhizobia were positive for both genes. Among non-nodulating, 48 out of 48 were nodC-negative. The results demonstrated the efficiency of this new method that can be completed in a day, to save time and money in RNB isolation projects, fulfilling Koch's postulate, and separating rhizobia from non-rhizobial bacteria. |
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Palavras-Chave: |
Koch's postulates; Nódulo da raiz. |
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Thesagro: |
Bactéria; Leguminosa. |
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Thesaurus NAL: |
Legumes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 2106246
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008 2019 bl uuuu u00u1 u #d
022 $a2452-2198
024 7 $a10.1016/j.rhisph.2019.100144$2DOI
100 1 $aSILVA, V. B. da
245 $aFast and efficient symbiotic gene-based duplex PCR approach for the preliminary selection of legume root nodule bacteria.$h[electronic resource]
260 $c2019
520 $aThe isolation of root nodule bacteria (RNB) usually lead to obtainment of several non-rhizobia, mainly those fastgrowing bacteria. The plant-authentication experiments to select the nodulating bacteria are time consuming and can be ruined in case of nodulation of negative controls. To speed up this step in rhizobiology research a fast, simple and efficient duplex PCR assay for the amplification of nodC and nifH genes in was developed aiming to separate rhizobia and non-rhizobia in RNB culture collections. The method was optimized with 43 reference strains and was applied to a new culture collection for validation. Considering all bacteria, 106 out of 109 nodulating rhizobia were positive for both genes. Among non-nodulating, 48 out of 48 were nodC-negative. The results demonstrated the efficiency of this new method that can be completed in a day, to save time and money in RNB isolation projects, fulfilling Koch's postulate, and separating rhizobia from non-rhizobial bacteria.
650 $aLegumes
650 $aBactéria
650 $aLeguminosa
653 $aKoch's postulates
653 $aNódulo da raiz
700 1 $aSILVA, A. F. da
700 1 $aSILVA, T. R. da
700 1 $aSANTOS, J. W. M. dos
700 1 $aSILVA, J. F. da
700 1 $aSOUZA, A. P. de
700 1 $aFREITAS, A. D. S. de
700 1 $aFERNANDES JUNIOR, P. I.
773 $tRhizosphere$gv. 10, 2019.
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