01846naa a2200289 a 450000100080000000500110000800800410001902200140006002400380007410000200011224501490013226000090028152009950029065000120128565000140129765000150131165300220132665300200134870000200136870000200138870000250140870000200143370000200145370000250147370000280149877300300152621062462020-01-21       2019    bl uuuu     u00u1 u    #d  a2452-21987 a10.1016/j.rhisph.2019.1001442DOI1 aSILVA, V. B. da  aFast and efficient symbiotic gene-based duplex PCR approach for the preliminary selection of legume root nodule bacteria.h[electronic resource]  c2019  aThe isolation of root nodule bacteria (RNB) usually lead to obtainment of several non-rhizobia, mainly those fastgrowing bacteria. The plant-authentication experiments to select the nodulating bacteria are time consuming and can be ruined in case of nodulation of negative controls. To speed up this step in rhizobiology research a fast, simple and efficient duplex PCR assay for the amplification of nodC and nifH genes in was developed aiming to separate rhizobia and non-rhizobia in RNB culture collections. The method was optimized with 43 reference strains and was applied to a new culture collection for validation. Considering all bacteria, 106 out of 109 nodulating rhizobia were positive for both genes. Among non-nodulating, 48 out of 48 were nodC-negative. The results demonstrated the efficiency of this new method that can be completed in a day, to save time and money in RNB isolation projects, fulfilling Koch's postulate, and separating rhizobia from non-rhizobial bacteria.  aLegumes  aBactéria  aLeguminosa  aKoch's postulates  aNódulo da raiz1 aSILVA, A. F. da1 aSILVA, T. R. da1 aSANTOS, J. W. M. dos1 aSILVA, J. F. da1 aSOUZA, A. P. de1 aFREITAS, A. D. S. de1 aFERNANDES JUNIOR, P. I.  tRhizospheregv. 10, 2019.