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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
08/06/2018 |
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Data da última atualização: |
29/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOSCO, D. D.; SINSKI, I.; RITSCHEL, P. S.; CAMARGO, U. A.; FAJARDO, T. V. M.; HARAKAVA, R.; QUECINI, V. |
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Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; Umberto A. Camargo; THOR VINICIUS MARTINS FAJARDO, CNPUV; Ricardo Harakava; VERA MARIA QUECINI, CNPUV. |
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Título: |
Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Transgenic Research, p. 1-18, 2018. [Online] |
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DOI: |
https://doi.org/10.1007/s11248-018-0082-1 |
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Idioma: |
Português |
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Conteúdo: |
Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis MenosIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and... Mostrar Tudo |
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Palavras-Chave: |
Fungus; Pathogenesis related protein 5. |
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Thesaurus Nal: |
Chitinase; Epigenetics; Grapevine leafroll-associated virus 3; Vitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178409/1/s11248-018-0082-1-Artigo-v-publicada-Metilacao-DNA-Transgenic-Research-06-06-18.pdf
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Marc: |
LEADER 02824naa a2200277 a 4500
001 2092380
005 2019-04-29
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11248-018-0082-1$2DOI
100 1 $aBOSCO, D. D.
245 $aExpression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.$h[electronic resource]
260 $c2018
520 $aIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis
650 $aChitinase
650 $aEpigenetics
650 $aGrapevine leafroll-associated virus 3
650 $aVitis
653 $aFungus
653 $aPathogenesis related protein 5
700 1 $aSINSKI, I.
700 1 $aRITSCHEL, P. S.
700 1 $aCAMARGO, U. A.
700 1 $aFAJARDO, T. V. M.
700 1 $aHARAKAVA, R.
700 1 $aQUECINI, V.
773 $tTransgenic Research, p. 1-18, 2018. [Online]
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
02/09/1996 |
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Data da última atualização: |
06/09/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BARROS, N. N.; KAWAS, J. R.; FREIRE, L. C. L.; ARAUJO FILHO, J. A. de; SHELTON, J. M.; JOHNSON, W. L. |
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Afiliação: |
NELSON NOGUEIRA BARROS, CNPC; JOÃO AMBRÓSIO DE ARAÚJO FILHO, CNPC. |
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Título: |
Digestibility and intake of various native and introduced forages by goats and hair sheep in Northeast Brazil. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
In: REUNIÃO TÉCNICO-CIENTÍFICA DO PROGRAMA DE APOIO A PESQUISA COLABORATIVA DE PEQUENOS RUMINANTES, 1., 1986. Sobral. Anais... Sobral: EMBRAPA-CNPC: SR-CRSP, 1986. p. 219-233. (EMBRAPA-CNPC. Documentos, 6). |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Results from a series of studies conducted at the EMBRAPA/CNPA were used to evaluate the quality of various native and introduced forages fed to goats and tropical hair sheep. Reduced forage quality was sttributed to a decreased dry matter intake and digestibility, decreases nitrogen content and intake appeared to be associated more with an increase in cell wall components, and a reduction in digestibility were affected by stage of maturity and forage species. Dry matter intake was lower for the grasses and digestibility was lower for the range species. [Digestibilidade e consumo de várias forragens nativas e introduzidas para cabras e ovelhas lanadas no Nordeste do Brasil]. |
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Palavras-Chave: |
Brasil; Foods for animals; Nordeste; Northeast. |
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Thesagro: |
Alimento para animal; Caprino; Consumo; Digestibilidade; Forragem; Nutrição animal; Ovino; Pastagem nativa. |
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Thesaurus NAL: |
Animal nutrition; Brazil; Digestibility; Feed intake; Forage; Goats; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/56854/1/AAC-Digestibility-and-intake.pdf
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Marc: |
LEADER 01922nam a2200397 a 4500
001 1514540
005 2021-09-06
008 1986 bl uuuu u00u1 u #d
100 1 $aBARROS, N. N.
245 $aDigestibility and intake of various native and introduced forages by goats and hair sheep in Northeast Brazil.$h[electronic resource]
260 $aIn: REUNIÃO TÉCNICO-CIENTÍFICA DO PROGRAMA DE APOIO A PESQUISA COLABORATIVA DE PEQUENOS RUMINANTES, 1., 1986. Sobral. Anais... Sobral: EMBRAPA-CNPC: SR-CRSP, 1986. p. 219-233. (EMBRAPA-CNPC. Documentos$c1986
520 $aAbstract: Results from a series of studies conducted at the EMBRAPA/CNPA were used to evaluate the quality of various native and introduced forages fed to goats and tropical hair sheep. Reduced forage quality was sttributed to a decreased dry matter intake and digestibility, decreases nitrogen content and intake appeared to be associated more with an increase in cell wall components, and a reduction in digestibility were affected by stage of maturity and forage species. Dry matter intake was lower for the grasses and digestibility was lower for the range species. [Digestibilidade e consumo de várias forragens nativas e introduzidas para cabras e ovelhas lanadas no Nordeste do Brasil].
650 $aAnimal nutrition
650 $aBrazil
650 $aDigestibility
650 $aFeed intake
650 $aForage
650 $aGoats
650 $aSheep
650 $aAlimento para animal
650 $aCaprino
650 $aConsumo
650 $aDigestibilidade
650 $aForragem
650 $aNutrição animal
650 $aOvino
650 $aPastagem nativa
653 $aBrasil
653 $aFoods for animals
653 $aNordeste
653 $aNortheast
700 1 $aKAWAS, J. R.
700 1 $aFREIRE, L. C. L.
700 1 $aARAUJO FILHO, J. A. de
700 1 $aSHELTON, J. M.
700 1 $aJOHNSON, W. L.
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Embrapa Caprinos e Ovinos (CNPC) |
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