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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/11/2010 |
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Data da última atualização: |
05/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PASQUAL, M.; PIO, L. A. S; SILVA, S. de O. e; DAVIDE, L. C; SEREJO, J. A. dos S.; ROCHA, H. S. |
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Afiliação: |
MOACIR PASQUAL, UFLA; LEILA APARECIDA SALLES PIO, UFLA; SEBASTIÃO DE OLIVEIRA E SILVA; LISETE CHAMMA DAVIDE, UFLA; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; HERMINIO SOUZA ROCHA, CNPMF. |
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Título: |
Chromosome doubling in musa acuminata colla. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM. |
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Volume: |
v.2 |
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Páginas: |
p. 757. |
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Idioma: |
Inglês |
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Notas: |
PDF. S18.201. |
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Conteúdo: |
The aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness
of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 tetraploid and 6 mixoploid plants by three different techniques. Anatomical and ultra structural analysis made possible screening of diploids. Nevertheless, the distinction between tetraploid and mixoploids was not possible. MenosThe aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness
of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 ... Mostrar Tudo |
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Palavras-Chave: |
Cultivares. |
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Thesagro: |
Banana. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02437naa a2200241 a 4500
001 1866599
005 2011-04-05
008 2010 bl uuuu u00u1 u #d
100 1 $aPASQUAL, M.
245 $aChromosome doubling in musa acuminata colla.
260 $c2010
300 $ap. 757. v.2
490 $vv.2
500 $aPDF. S18.201.
520 $aThe aim in this research work was the identification of polyploidy by means of stomata analysis and flow citometry, effects of poly and mixoploidy over anatomic and ultra structural traits on the following banana cultivars Malbut, Ouro, Lidi e Thong Dok Mak. Polyplodization was induced by colchicine treatment, in 0, 2,5; 7,5 e 12,5 mM concentrations, for 24 and 48 hours and by orizaline, in 0, 10, 30 e 50 ?M concentrations, for 4 and 7 days. Stomata analysis consisted of measurements of size and density by scanning electron microscopy (SEM). For the flow citometry analisys a small piece of the tissue was grinded and dyed with propidium iodide. Citogenetics analysis was performed using root tips pre treated with 8-HQ 500 mg.L-1 solution, during 3 hours, fixed in Carnoy and subsequently submitted to conventional squeezing method. Anatomic and ultra structural analysis was done in all the diploid, mixoploid and tetraploid plants of ?Lidi?, ?Ouro? and ?Tong Dok Mak?. Young leaf samples were traditionally prepared for transmission Electron Microscopy (TEM). Semi (>100nm) and ultra thin (<100nm) sessions were cut from the tissues. The semi thin sessions were analyzed by light microscope, measuring length and width of chlorophyll and air parenchymas, of superior and inferior epidermis, thickness of the leaves sessions and number of chloroplastids. TEM was used to analyze the ultra thin sessions to characterize the chloroplasts. The main results generated were, identification of 4 tetraploid and 6 mixoploid plants by three different techniques. Anatomical and ultra structural analysis made possible screening of diploids. Nevertheless, the distinction between tetraploid and mixoploids was not possible.
650 $aBanana
653 $aCultivares
700 1 $aPIO, L. A. S
700 1 $aSILVA, S. de O. e
700 1 $aDAVIDE, L. C
700 1 $aSEREJO, J. A. dos S.
700 1 $aROCHA, H. S.
773 $tIn: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Pantanal. |
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Data corrente: |
11/01/2005 |
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Data da última atualização: |
11/01/2005 |
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Autoria: |
ABREU, U. G. P. de; ROSA, A. do N.; SERENO, J. R. B.; SANTOS, S. A. |
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Afiliação: |
Embrapa Pantanal (Corumbá, MS); Embrapa Gado de Corte (Campo Grande, MS). |
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Título: |
Melhoramento genético. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: CARDOSO, E. L. (Ed. Tecn.). Gado de corte no Pantanal: o produtor pergunta, a Embrapa responde. Brasília: Informação Tecnológica, 2004. (Coleção 500 perguntas, 500 respostas). |
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Páginas: |
p.171-185. |
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Idioma: |
Português |
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Palavras-Chave: |
Bovino de corte; Brasil; Husbandry; Melhoramento genético. |
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Thesagro: |
Bovino; Melhoramento Genético Animal; Pecuária. |
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Thesaurus NAL: |
animal breeding; beef cattle; Brazil; Pantanal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00864naa a2200289 a 4500
001 1811481
005 2005-01-11
008 2004 bl uuuu u00u1 u #d
100 1 $aABREU, U. G. P. de
245 $aMelhoramento genético.
260 $c2004
300 $ap.171-185.
650 $aanimal breeding
650 $abeef cattle
650 $aBrazil
650 $aPantanal
650 $aBovino
650 $aMelhoramento Genético Animal
650 $aPecuária
653 $aBovino de corte
653 $aBrasil
653 $aHusbandry
653 $aMelhoramento genético
700 1 $aROSA, A. do N.
700 1 $aSERENO, J. R. B.
700 1 $aSANTOS, S. A.
773 $tIn: CARDOSO, E. L. (Ed. Tecn.). Gado de corte no Pantanal: o produtor pergunta, a Embrapa responde. Brasília: Informação Tecnológica, 2004. (Coleção 500 perguntas, 500 respostas).
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Embrapa Pantanal (CPAP) |
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