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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Amazônia Ocidental. |
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Data corrente: |
08/02/2023 |
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Data da última atualização: |
06/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, T. F.; ARAÚJO JÚNIOR, M. B. de; PERES, E. G.; SOUZA, M. P.; SILVA, F. M. A. da; MEDEIROS, L. S. de; SOUZA, A. D. L. de; SOUZA, A. Q. L. de S.; YAMAGISHI, M. E. B.; SILVA, G. F. da; KOOLEN, H. H. F.; QUEIROZ, M. V. de. |
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Afiliação: |
THIAGO F. SOUSA, UNIVERSIDADE DO ESTADO DO AMAZONAS, UNIVERSIDADE FEDERAL DE VIÇOSA; MOYSÉS B. DE ARAÚJO JÚNIOR, UNIVERSIDADE DO ESTADO DO AMAZONAS, UNIVER-SIDADE FEDERAL DO AMAZONAS; ELDRINEI G. PERES, UNIVERSIDADE DO ESTADO DO AMAZONAS, UNIVERSIDADE FEDERAL DO AMAZONAS; MAYANE P. SOUZA, UNIVERSIDADE FEDERAL DO AMAZONAS; FELIPE M. A. DA SILVA, UNIVERSIDADE FEDERAL DO AMAZONAS; LÍVIA S. DE MEDEIROS, UNIVERSIDADE FEDERAL DE SÃO PAULO; AFONSO D. L. DE SOUZA, UNIVERSIDADE FEDERAL DO AMAZONAS; ANTONIA Q. L. DE SOUZA, UNIVERSIDADE FEDERAL DO AMAZONAS; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; GILVAN FERREIRA DA SILVA, CPAA; HECTOR H. F. KOOLEN, UNIVERSIDADE DO ESTADO DO AMAZONAS; MARISA V. DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA. |
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Título: |
Discovery of dual PKS involved in sclerotiorin biosynthesis in Penicillium meliponae using genome mining and gene knockout. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Archives of Microbiology, v. 205, n. 2, 75, Feb. 2023. |
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DOI: |
https://doi.org/10.1007/s00203-023-03414-1 |
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Idioma: |
Inglês |
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Conteúdo: |
In this work, the gene knockout of two PKS together with mass spectrometry data provide evidence of a BGC for the production of sclerotiorin and sclerotiorin-like metabolites; on the other hand, the biosynthesis pathway for these molecules is proposed. |
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Palavras-Chave: |
Biosynthetic gene clusters; Fungal BGC; Natural products; Penicillium meliponae; Produtos naturais. |
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Thesagro: |
Fungo. |
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Thesaurus Nal: |
Genome mining; Polyketides. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01372naa a2200361 a 4500
001 2151585
005 2023-09-06
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00203-023-03414-1$2DOI
100 1 $aSOUSA, T. F.
245 $aDiscovery of dual PKS involved in sclerotiorin biosynthesis in Penicillium meliponae using genome mining and gene knockout.$h[electronic resource]
260 $c2023
520 $aIn this work, the gene knockout of two PKS together with mass spectrometry data provide evidence of a BGC for the production of sclerotiorin and sclerotiorin-like metabolites; on the other hand, the biosynthesis pathway for these molecules is proposed.
650 $aGenome mining
650 $aPolyketides
650 $aFungo
653 $aBiosynthetic gene clusters
653 $aFungal BGC
653 $aNatural products
653 $aPenicillium meliponae
653 $aProdutos naturais
700 1 $aARAÚJO JÚNIOR, M. B. de
700 1 $aPERES, E. G.
700 1 $aSOUZA, M. P.
700 1 $aSILVA, F. M. A. da
700 1 $aMEDEIROS, L. S. de
700 1 $aSOUZA, A. D. L. de
700 1 $aSOUZA, A. Q. L. de S.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aSILVA, G. F. da
700 1 $aKOOLEN, H. H. F.
700 1 $aQUEIROZ, M. V. de
773 $tArchives of Microbiology$gv. 205, n. 2, 75, Feb. 2023.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
22/11/2021 |
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Data da última atualização: |
22/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS‑TIERNO, R.; GARCIA, R.; FONSECA, E.; FALEIRO, F. G.; MOREIRA, D.; PACHECO, G.; MANSUR, E. |
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Afiliação: |
RAPHAELA SANTOS‑TIERNO; RENATA GARCIA; EDUARDO FONSECA; FABIO GELAPE FALEIRO, CPAC; DAVYSON MOREIRA; GEORGIA PACHECO; ELISABETH MANSUR. |
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Título: |
Light quality and explant type modulate growth, antioxidant properties and bioactive compounds production of calluses of Passiflora setacea cv BRS Pérola do Cerrado. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 147, p. 635-646, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Passiflora species have high industrial and medicinal relevance, due to presence of specific metabolites and their recognized pharmacological activities. In vitro systems allow the modulation of secondary metabolites production according to culture conditions. In this work, we report the effect of different light emitting diodes (LED) on induction, metabolite production, and antioxidant properties of calluses derived from stem and leaf segments of P. setacea cv BRS Pérola do Cerrado. Friable calluses were induced on MSM medium supplemented with picloram, under different light qualities (white fluorescent lamps or different types of LEDs) or in the dark. Light quality contributed more significantly for biomass accumulation than the other variables. Callus formation was observed in response to all treatments, although the highest biomass accumulation was induced by red LED. Chromatographic analyses indicated that blue LED induced the highest production of bioactive substances. All samples displayed low antioxidant potential by the DPPH assay, but showed a high capacity to chelate iron. The activity of antioxidant enzymes was also evaluated. The incubation under red LED caused an increase on the activity of superoxide dismutase, catalase and ascorbate peroxidase in calluses derived from internodal segments induced under red LED. This is the first study evaluating the effect of lighting conditions in in vitro systems of P. setacea cv BRS Pérola do Cerrado, thus opening new possibilities of study and utilization. MenosPassiflora species have high industrial and medicinal relevance, due to presence of specific metabolites and their recognized pharmacological activities. In vitro systems allow the modulation of secondary metabolites production according to culture conditions. In this work, we report the effect of different light emitting diodes (LED) on induction, metabolite production, and antioxidant properties of calluses derived from stem and leaf segments of P. setacea cv BRS Pérola do Cerrado. Friable calluses were induced on MSM medium supplemented with picloram, under different light qualities (white fluorescent lamps or different types of LEDs) or in the dark. Light quality contributed more significantly for biomass accumulation than the other variables. Callus formation was observed in response to all treatments, although the highest biomass accumulation was induced by red LED. Chromatographic analyses indicated that blue LED induced the highest production of bioactive substances. All samples displayed low antioxidant potential by the DPPH assay, but showed a high capacity to chelate iron. The activity of antioxidant enzymes was also evaluated. The incubation under red LED caused an increase on the activity of superoxide dismutase, catalase and ascorbate peroxidase in calluses derived from internodal segments induced under red LED. This is the first study evaluating the effect of lighting conditions in in vitro systems of P. setacea cv BRS Pérola do Cerrado, thus opening new possi... Mostrar Tudo |
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Palavras-Chave: |
Diodos emissores de luz; DPPH; Enzimas antioxidantes; Fitoquímica; Quelação de ferro. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02350naa a2200253 a 4500
001 2136409
005 2021-11-22
008 2021 bl uuuu u00u1 u #d
100 1 $aSANTOS‑TIERNO, R.
245 $aLight quality and explant type modulate growth, antioxidant properties and bioactive compounds production of calluses of Passiflora setacea cv BRS Pérola do Cerrado.$h[electronic resource]
260 $c2021
520 $aPassiflora species have high industrial and medicinal relevance, due to presence of specific metabolites and their recognized pharmacological activities. In vitro systems allow the modulation of secondary metabolites production according to culture conditions. In this work, we report the effect of different light emitting diodes (LED) on induction, metabolite production, and antioxidant properties of calluses derived from stem and leaf segments of P. setacea cv BRS Pérola do Cerrado. Friable calluses were induced on MSM medium supplemented with picloram, under different light qualities (white fluorescent lamps or different types of LEDs) or in the dark. Light quality contributed more significantly for biomass accumulation than the other variables. Callus formation was observed in response to all treatments, although the highest biomass accumulation was induced by red LED. Chromatographic analyses indicated that blue LED induced the highest production of bioactive substances. All samples displayed low antioxidant potential by the DPPH assay, but showed a high capacity to chelate iron. The activity of antioxidant enzymes was also evaluated. The incubation under red LED caused an increase on the activity of superoxide dismutase, catalase and ascorbate peroxidase in calluses derived from internodal segments induced under red LED. This is the first study evaluating the effect of lighting conditions in in vitro systems of P. setacea cv BRS Pérola do Cerrado, thus opening new possibilities of study and utilization.
653 $aDiodos emissores de luz
653 $aDPPH
653 $aEnzimas antioxidantes
653 $aFitoquímica
653 $aQuelação de ferro
700 1 $aGARCIA, R.
700 1 $aFONSECA, E.
700 1 $aFALEIRO, F. G.
700 1 $aMOREIRA, D.
700 1 $aPACHECO, G.
700 1 $aMANSUR, E.
773 $tPlant Cell, Tissue and Organ Culture$gv. 147, p. 635-646, 2021.
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