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| Registros recuperados : 57 | |
| 2. |  | PEREIRA-CARVALHO, R. C.; CHARCHAR, J. M.; BOITEUX, L. S. Avaliação de acessos de alface (Lactuca sativa) para resistência simultânea a Meloidogyne javanica e M. incognita. Horticultura Brasileira, Brasília, DF, v. 25, n. 1, p. s95, ago. 2007. Suplemento. Resumo 512. Trabalho apresentado no 47. Congresso Brasileiro de Olericultura, 4. Simpósio Brasileiro sobre Cucurbitáceas, 2007. Porto Seguro.| Biblioteca(s): Embrapa Hortaliças. |
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| 3. | | BATISTA, J. G.; RESENDE, R. O.; LIMA, M. F.; PEREIRA-CARVALHO, R. C. Prospecção de espécies de cucumovírus, potyvírus e tobamovírus em mudas de espécies arbóreas. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS COLHEITA, 2., 2015, São Pedro, SP. Fitopatologia de precisão: fronteiras da ciência: anais. São Pedro, SP: Sociedade Brasileira de Fitopatologia, 2015. Resumo 354-2.| Biblioteca(s): Embrapa Hortaliças. |
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| 4. | | BATISTA, J. G.; RESENDE, R. O.; LIMA, M. F.; BOITEUX, L. S.; PEREIRA-CARVALHO, R. C. Prospecção da ocorrência natural de três gêneros virais (Cucumovírus, Potyvirus e Tobamovírus) em mudas de espécies arbóreas e arbustivas. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 p. Resumo 654_1.| Biblioteca(s): Embrapa Hortaliças. |
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| 5. | | BLAWID, R.; MORGADO, F. S.; SOUZA, C. A.; BOITEUX, L. S.; PEREIRA-CARVALHO, R. C. Fluorescence in situ hybridization analysis of endosymbiont genera reveals novel infection patterns in a tomato-infesting Bemisia tabaci population from Brazil. Tropical Plant Pathology, Brasília, DF, v. 40, n. 4, p. 233-243, Aug. 2015.| Biblioteca(s): Embrapa Hortaliças. |
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| 7. | | BATISTA, J. G.; RESENDE, R. O.; BOITEUX, L. S.; LIMA, M. F.; PEREIRA-CARVALHO, R. C. Avaliação do potencial de espécies arbóreas como hospedeiras de Tomato chlorotic mottle virus (ToCMoV) e Groundnut ringspot virus (GRSV). In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS COLHEITA, 2., 2015, São Pedro, SP. Fitopatologia de precisão: fronteiras da ciência: anais. São Pedro, SP: Sociedade Brasileira de Fitopatologia, 2015. Resumo 354-1.| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | ABREU, H.; FONSECA, M. E. de N.; PEREIRA-CARVALHO, R. C.; BOITEUX, L. S. Tomato chlorosis virus on the weed Physalis angulata within tomato fields in São Paulo State, Brazil. Tropical Plant Pathology, Brasília, DF, v. 37, 2012. 1 CD-ROM. Suplemento. Edição do 45º Congresso Brasileiro de Fitopatologia, 2012, Manaus. Resumo 772.| Biblioteca(s): Embrapa Hortaliças. |
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| 13. | | PEREIRA-CARVALHO, R. C.; BOITEUX, L. S.; NOGUEIRA, I.; FERNANDES-ACIOLI, N. A. N.; FONSECA-BOITEUX, M. E. de N. Evaluation of tomato germplasm reaction to Tomato chlorosis virus. Tropical Plant Pathology, Brasília, DF, v. 36, p. 1412, 2011. 1 CD-ROM. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 1554.| Biblioteca(s): Embrapa Hortaliças. |
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| 14. | | PEREIRA-CARVALHO, R. C.; DIAZ-PENDÓN, J. A.; FERNANDÉZ-MUÑOZ, R.; BOITEUX, L. S.; MORIONES, E.; RESENDE, R. O. Estudo do mecanismo de resistência a begomovirus na linhagem de tomate 'TX-468' e seu efeito na dispersão do Tomato yellow leaf curl virus por Bemisia tabaci. Tropical Plant Pathology, Brasília, DF, v. 33, p. S294, ago. 2008. Suplemento. Resumo VIR 039. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008.| Biblioteca(s): Embrapa Hortaliças. |
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| 15. | | NOGUEIRA, I.; FONSECA, M. E. de N.; RESENDE, R. O.; BLAWID, R.; INOUE=NAGATA, A. K.; PEREIRA-CARVALHO, R. C. Diversidade de endossimbiontes em Bemisia tabaci coletadadas no tomateiro e diferentes hospedeiras em localidades do Centro Oeste. Tropical Plant Pathology, Brasília, DF, v. 37, 2012. 1 CD-ROM. Suplemento. Edição do 45º Congresso Brasileiro de Fitopatologia, 2012, Manaus. Resumo 805.| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | PEREIRA-CARVALHO, R. C.; FONSECA, M. E. N.; NOGUEIRA, L.; FERNANDES-ACIOLI, N. A. N.; BOITEUX, L. S. Inheritance of a tolerant reaction to Tomato chlorosis crinivirus in tomato. Tropical Plant Pathology, Brasília, DF, v. 36, p. 1413, 2011. 1 CD-ROM. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 1555.| Biblioteca(s): Embrapa Hortaliças. |
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| 17. | | FONSECA-BOITEUX, M. E. de N.; BOITEUX, L. S.; ABREU, H.; NOGUEIRA, I.; PEREIRA-CARVALHO, R. C. Physalis angulata: a new natural host of tomato chlorosis virus in Brasil. Plant Disease, Saint Paul, v. 97, n. 5, p. 692, May 2013. Disease notes| Biblioteca(s): Embrapa Hortaliças. |
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| 18. | | DUARTE, M. F.; FONSECA, M. E. N.; BOITEUX, L. S.; COSTA, H.; RIBEIRO, B. M.; MELO, F. L.; PEREIRA-CARVALHO, R. C. Identification of Physalis angulata (Solanaceae) as a Natural Alternative Weed Host of Tomato Severe Rugose Virus in Brazil Plant Disease, v. 104, n. 2, p. 600, Feb. 2020.| Biblioteca(s): Embrapa Hortaliças. |
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| 19. | | PEREIRA-CARVALHO, R. C.; BOITEUX, L. S.; FONSECA, M. E. N.; MORIONES, E.; FERNANDEZ-MUÑOZ, R.; DIAZ-PENDÓN, J.A.; CHARCHAR, J. M.; RESENDE, R. O. Identification in solanum (section lycopersicon) species of genetic sources combining resistance to root-knot nematodes, monopartite and bipartite begomovirus. Virus Reviews & Research, São Paulo, v. 14, Supl. 1, p. 247-248, nov. 2009. Suplemento 1. Resumo.Trabalho apresentado no 20. National Meeting of Virology, 2009, Brasília, Distrito Federal, Brasil.| Biblioteca(s): Embrapa Hortaliças. |
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| 20. | | VIEIRA, B. G.; FARIAS, P. C.; NOGUEIRA, L.; PEREIRA-CARVALHO, R. C.; RIBEIRO, S. G.; FONSECA, M. E. N.; LACORTE, C.; RESENDE, R. O.; BOITEUX, L. S. Identificação de análogos de locus Ty-3 em acessos em Solanum peruvianum e eficiência contra tomato severe rugose vírus e tomato yellow vein streak virus. Tropical Plant Pathology, Brasília, DF, v. 36, p. 1410, 2011. 1 CD-ROM. Resumo 1533. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 57 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/09/2020 |
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Data da última atualização: |
16/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NERY, F. M. B.; MELO, F. L.; BOITEUX, L. S.; RIBEIRO, S. da G.; RESENDE, R. O.; ORÍLIO, A. F.; BATISTA, J. G.; LIMA, M. F.; PEREIRA-CARVALHO, R. C. |
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Afiliação: |
FLÁVIA M. B. NERY, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; LEONARDO SILVA BOITEUX, CNPH; SIMONE DA GRACA RIBEIRO, Cenargen; RENATO O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANELISE F. ORÍLIO, UNIVERSIDADE DE BRASÍLIA; JOSIANE G. BATISTA, UNIVERSIDADE DE BRASÍLIA; MIRTES FREITAS LIMA, CNPH; RITA C. PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Molecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the Order Picornavirales. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Viruses, v. 12, n. 9, 950, 2020 |
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DOI: |
https://doi.org/10.3390/v12090950 |
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Idioma: |
Inglês |
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Conteúdo: |
In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. MenosIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel viru... Mostrar Tudo |
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Palavras-Chave: |
HDaV1; HDaV2; Virome. |
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Thesagro: |
Hovenia Dulcis. |
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Thesaurus NAL: |
Metagenomics; Picornavirales; Viruses. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219297/1/ARTIGO-MolecularCharacterizationHoveniaDulcis.pdf
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Marc: |
LEADER 02445naa a2200313 a 4500
001 2128264
005 2020-12-16
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/v12090950$2DOI
100 1 $aNERY, F. M. B.
245 $aMolecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2)$bnew tentative species within the Order Picornavirales.$h[electronic resource]
260 $c2020
520 $aIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.
650 $aMetagenomics
650 $aPicornavirales
650 $aViruses
650 $aHovenia Dulcis
653 $aHDaV1
653 $aHDaV2
653 $aVirome
700 1 $aMELO, F. L.
700 1 $aBOITEUX, L. S.
700 1 $aRIBEIRO, S. da G.
700 1 $aRESENDE, R. O.
700 1 $aORÍLIO, A. F.
700 1 $aBATISTA, J. G.
700 1 $aLIMA, M. F.
700 1 $aPEREIRA-CARVALHO, R. C.
773 $tViruses$gv. 12, n. 9, 950, 2020
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Embrapa Hortaliças (CNPH) |
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