02445naa a2200313 a 450000100080000000500110000800800410001902400430006010000190010324501690012226000090029152015390030065000170183965000190185665000120187565000190188765300100190665300100191665300110192670000160193770000190195370000220197270000190199470000190201370000190203270000160205170000280206777300360209521282642020-12-16       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.3390/v120909502DOI1 aNERY, F. M. B.  aMolecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2)bnew tentative species within the Order Picornavirales.h[electronic resource]  c2020  aIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.  aMetagenomics  aPicornavirales  aViruses  aHovenia Dulcis  aHDaV1  aHDaV2  aVirome1 aMELO, F. L.1 aBOITEUX, L. S.1 aRIBEIRO, S. da G.1 aRESENDE, R. O.1 aORÍLIO, A. F.1 aBATISTA, J. G.1 aLIMA, M. F.1 aPEREIRA-CARVALHO, R. C.  tVirusesgv. 12, n. 9, 950, 2020