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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Tabuleiros Costeiros. |
Data corrente: |
25/01/2008 |
Data da última atualização: |
10/11/2010 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
SÁ, J. L. de; SÁ, C. O. de; SOBRAL, P. H. M.; COSTA, C. X.; SILVA, A. V. C. da; MUNIZ, E. N.; CIPRIANO, L. W.; CORREIA NETO, J. |
Título: |
Análise sensorial e microbiológica da carne ovina submetida a diferentes formas de conservação no pós-abate. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 3; FEIRA NACIONAL DO AGRONEGÓCIO DA CAPRINO-OVINOCULTURA DE CORTE, 2007, João Pessoa. Anais... João Pessoa: EMEPA-PB, 2007. 1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Análise sensorial; Conservação da carne; Ovino de corte. |
Thesagro: |
Abate; Carne; Conservação; Microbiologia de Alimento. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00983naa a2200277 a 4500 001 1534219 005 2010-11-10 008 2007 bl uuuu u00u1 u #d 100 1 $aSÁ, J. L. de 245 $aAnálise sensorial e microbiológica da carne ovina submetida a diferentes formas de conservação no pós-abate.$h[electronic resource] 260 $c2007 650 $aAbate 650 $aCarne 650 $aConservação 650 $aMicrobiologia de Alimento 653 $aAnálise sensorial 653 $aConservação da carne 653 $aOvino de corte 700 1 $aSÁ, C. O. de 700 1 $aSOBRAL, P. H. M. 700 1 $aCOSTA, C. X. 700 1 $aSILVA, A. V. C. da 700 1 $aMUNIZ, E. N. 700 1 $aCIPRIANO, L. W. 700 1 $aCORREIA NETO, J. 773 $tIn: SIMPÓSIO INTERNACIONAL SOBRE CAPRINOS E OVINOS DE CORTE, 3; FEIRA NACIONAL DO AGRONEGÓCIO DA CAPRINO-OVINOCULTURA DE CORTE, 2007, João Pessoa. Anais... João Pessoa: EMEPA-PB, 2007. 1 CD-ROM.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
20/12/2023 |
Data da última atualização: |
20/12/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
AGUSTINI, B. C.; CORREA, O. L. dos S.; OSTER, A. H.; SILVA, G. A. |
Afiliação: |
BRUNA CARLA AGUSTINI, CNPUV; ODINELI LOUZADA DOS SANTOS CORREA, CNPUV; ANDREIA HANSEN OSTER, CNPUV; GILDO ALMEIDA SILVA, EMBRAPA UVA E VINHO. |
Título: |
Yeast as a biological agent in the control of botrytis cinerea. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 32., 2023, Foz do Iguaçú, PR. Anais... FOz do Iguaçú: SBM, 18 a 22 de out. 2023. p. 1487-2. |
Idioma: |
Inglês |
Conteúdo: |
Gray mold of grapes, also called Botrytis bunch rot is caused by the fungus Botrytis cinerea and is found in practically all vineyards in the world, causing serious reductions in the quality and quantity of grapes produced. The control of this disease is based on chemical fungicides applications associated with grapevine management techniques. However, during grape maturation, the use of chemical fungicides is restricted due to the pre-harvest interval. Hence, an alternative product for controlling these pests would provide relief for producers. The use of biological control agents may be a promising option to attend the growing demands for healthy products and sustainable management techniques that respect the environment and reduce the use of pesticides. In previous work, the antagonism of a Saccharomyces cerevisiae strain, present in the CMIA Collection of Embrapa Grape and Wine, against B. cinerea has already been demonstrated by the production of diffusible substances in solid culture medium. In order to understand the mechanisms involved in this biocontrol, the present work had the objective of growing this yeast strain in liquid medium and evaluate its supernatant without active cells against the same fungus. To this end, the S. cerevisiae strain was grown in a complex medium for 96 hours at 28°C and three treatments (in duplicate) were carried out: A) Medium was centrifuged and its supernatant was autoclaved; B) Medium was centrifuged and the supernatant was filtered at 0.22µm; C) Full medium (without centrifugation) was autoclaved. As positive control, the target fungi were inoculated into the same culture medium used for yeast growth. Each of the treatments was inoculated with two discs (0.5 cm each) of the fungus mycelium. After inoculation, mycelial growth was observed daily by visual inspection for 96 hours. The results indicate that the biocontrol compound appears to be thermolabile, once 48 hours after the start of the test, the fungus began to grow in the treatments A and C, while treatment B did not show proliferation until 96 hours. This outcome reinforces the need for characterization and identification of the metabolites produced by the yeast to unravel the mechanisms of action involved in the biocontrol observed and, consequently, support new bioinput development. MenosGray mold of grapes, also called Botrytis bunch rot is caused by the fungus Botrytis cinerea and is found in practically all vineyards in the world, causing serious reductions in the quality and quantity of grapes produced. The control of this disease is based on chemical fungicides applications associated with grapevine management techniques. However, during grape maturation, the use of chemical fungicides is restricted due to the pre-harvest interval. Hence, an alternative product for controlling these pests would provide relief for producers. The use of biological control agents may be a promising option to attend the growing demands for healthy products and sustainable management techniques that respect the environment and reduce the use of pesticides. In previous work, the antagonism of a Saccharomyces cerevisiae strain, present in the CMIA Collection of Embrapa Grape and Wine, against B. cinerea has already been demonstrated by the production of diffusible substances in solid culture medium. In order to understand the mechanisms involved in this biocontrol, the present work had the objective of growing this yeast strain in liquid medium and evaluate its supernatant without active cells against the same fungus. To this end, the S. cerevisiae strain was grown in a complex medium for 96 hours at 28°C and three treatments (in duplicate) were carried out: A) Medium was centrifuged and its supernatant was autoclaved; B) Medium was centrifuged and the supernatant was filtered... Mostrar Tudo |
Palavras-Chave: |
Bionput; Grapevine; Yeast. |
Thesagro: |
Botrytis Cinerea. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160133/1/Agustini-CBM-2023-1487-2.pdf
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Marc: |
LEADER 02962nam a2200193 a 4500 001 2160133 005 2023-12-20 008 2023 bl uuuu u00u1 u #d 100 1 $aAGUSTINI, B. C. 245 $aYeast as a biological agent in the control of botrytis cinerea.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 32., 2023, Foz do Iguaçú, PR. Anais... FOz do Iguaçú: SBM, 18 a 22 de out. 2023. p. 1487-2.$c2023 520 $aGray mold of grapes, also called Botrytis bunch rot is caused by the fungus Botrytis cinerea and is found in practically all vineyards in the world, causing serious reductions in the quality and quantity of grapes produced. The control of this disease is based on chemical fungicides applications associated with grapevine management techniques. However, during grape maturation, the use of chemical fungicides is restricted due to the pre-harvest interval. Hence, an alternative product for controlling these pests would provide relief for producers. The use of biological control agents may be a promising option to attend the growing demands for healthy products and sustainable management techniques that respect the environment and reduce the use of pesticides. In previous work, the antagonism of a Saccharomyces cerevisiae strain, present in the CMIA Collection of Embrapa Grape and Wine, against B. cinerea has already been demonstrated by the production of diffusible substances in solid culture medium. In order to understand the mechanisms involved in this biocontrol, the present work had the objective of growing this yeast strain in liquid medium and evaluate its supernatant without active cells against the same fungus. To this end, the S. cerevisiae strain was grown in a complex medium for 96 hours at 28°C and three treatments (in duplicate) were carried out: A) Medium was centrifuged and its supernatant was autoclaved; B) Medium was centrifuged and the supernatant was filtered at 0.22µm; C) Full medium (without centrifugation) was autoclaved. As positive control, the target fungi were inoculated into the same culture medium used for yeast growth. Each of the treatments was inoculated with two discs (0.5 cm each) of the fungus mycelium. After inoculation, mycelial growth was observed daily by visual inspection for 96 hours. The results indicate that the biocontrol compound appears to be thermolabile, once 48 hours after the start of the test, the fungus began to grow in the treatments A and C, while treatment B did not show proliferation until 96 hours. This outcome reinforces the need for characterization and identification of the metabolites produced by the yeast to unravel the mechanisms of action involved in the biocontrol observed and, consequently, support new bioinput development. 650 $aBotrytis Cinerea 653 $aBionput 653 $aGrapevine 653 $aYeast 700 1 $aCORREA, O. L. dos S. 700 1 $aOSTER, A. H. 700 1 $aSILVA, G. A.
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