| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
07/03/2008 |
|
Data da última atualização: |
28/02/2024 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
|
Autoria: |
SOARES JÚNIOR, M. S.; GEBIN, P. F. C.; JUNQUEIRA, T. de L.; GOMES, V. A.; LACERDA, D. B. C. L.; BASSINELLO, P. Z. |
|
Afiliação: |
MANOEL S. SOARES JÚNIOR, UFG; PEDRO F. C. GEBIN, graduando UFG; THAÍS DE L. JUNQUEIRA, graduanda UFG; VINÍCIUS A. GOMES, graduando UFG; DIRACY B. C. L. LACERDA, mestranda UFG; PRISCILA ZACZUK BASSINELLO, CNPAF. |
|
Título: |
Aceitabilidade de pães de forma com diferentes teores de farelo de arroz torrado em substituição parcial à farinha de trigo. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Higiene Alimentar, São Paulo, v. 21, n. 150, p. 59-60, abr. 2007. Edição dos resumos III Congresso Latinoamericano, IX Brasileiro de Higienistas de Alimentos, II Encontro Nacional de Centros de Controle de Zoonose, I Encontro Nacional do Sistema Brasileiro de Inspeção de Produtos de Origem Animal, Porto Seguro, abr. 2007. |
|
Idioma: |
Português |
|
Conteúdo: |
O presente trabalho teve como objetivo substituir a farinha de trigo por diferentes teores de farelo de arroz torrado na formulação de pães de forma, com intuito de determinar qual teor de farelo de arroz torrado no pão de forma provocaria uma aceitabilidade mais próxima à do pão de forma somente com farinha de trigo. |
|
Palavras-Chave: |
Farelo de arroz; Pão de forma. |
|
Thesagro: |
Arroz; Farelo; Valor Nutritivo. |
|
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/216462/1/higienealimentar.pdf
|
|
Marc: |
LEADER 01329nam a2200229 a 4500
001 1216462
005 2024-02-28
008 2007 bl uuuu u00u1 u #d
100 1 $aSOARES JÚNIOR, M. S.
245 $aAceitabilidade de pães de forma com diferentes teores de farelo de arroz torrado em substituição parcial à farinha de trigo.$h[electronic resource]
260 $aHigiene Alimentar, São Paulo, v. 21, n. 150, p. 59-60, abr. 2007. Edição dos resumos III Congresso Latinoamericano, IX Brasileiro de Higienistas de Alimentos, II Encontro Nacional de Centros de Controle de Zoonose, I Encontro Nacional do Sistema Brasileiro de Inspeção de Produtos de Origem Animal, Porto Seguro, abr. 2007.$c2007
520 $aO presente trabalho teve como objetivo substituir a farinha de trigo por diferentes teores de farelo de arroz torrado na formulação de pães de forma, com intuito de determinar qual teor de farelo de arroz torrado no pão de forma provocaria uma aceitabilidade mais próxima à do pão de forma somente com farinha de trigo.
650 $aArroz
650 $aFarelo
650 $aValor Nutritivo
653 $aFarelo de arroz
653 $aPão de forma
700 1 $aGEBIN, P. F. C.
700 1 $aJUNQUEIRA, T. de L.
700 1 $aGOMES, V. A.
700 1 $aLACERDA, D. B. C. L.
700 1 $aBASSINELLO, P. Z.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
07/04/2010 |
|
Data da última atualização: |
07/04/2010 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
FARIAS, T. A.; ARAUJO, F. R.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; AZAMBUJA, A.; ROSINHA, G. M. S.; SOARES, C. O.; ELISEI, C. |
|
Afiliação: |
Thaís A. Farias, UFMS; FLABIO RIBEIRO ARAUJO, CNPGC; Ana Luíza A. R. Osório, UFMS; Kláudia S. G. Jorge, UFMS; Carlos A. N. Ramos, CNPGC; Ingrid I. F. Souza, CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; Alexandre Azambuja, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; CARINA ELISEI, CNPGC. |
|
Título: |
ELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. |
|
Páginas: |
1 p. |
|
Descrição Física: |
1 CD-ROM. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Bovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of anergic animals in advanced stages of the disease. MenosBovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of... Mostrar Tudo |
|
Thesagro: |
Diagnostico; Elisa; Mycobacterium Bovis; Sanidade Animal. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 02453naa a2200301 a 4500
001 1663370
005 2010-04-07
008 2009 bl uuuu u00u1 u #d
100 1 $aFARIAS, T. A.
245 $aELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis.
260 $c2009
300 $a1 p.$c1 CD-ROM.
520 $aBovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of anergic animals in advanced stages of the disease.
650 $aDiagnostico
650 $aElisa
650 $aMycobacterium Bovis
650 $aSanidade Animal
700 1 $aARAUJO, F. R.
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
700 1 $aSOUZA, I. I. F.
700 1 $aOLIVEIRA, R. H. M. de
700 1 $aAZAMBUJA, A.
700 1 $aROSINHA, G. M. S.
700 1 $aSOARES, C. O.
700 1 $aELISEI, C.
773 $tIn: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
