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| 2. |  | BRAGANÇA, J. C. A. de; COUTO JUNIOR, A. F.; SOUZA, V. V. de; MARTINS, E. de S.; GUIMARÃES, R. F.; CARVALHO JUNIOR, O. A. de. Análise espectral e a sua relação com características físicas dos solos do planalto do Oeste baiano. In: SIMPÓSIO BRASILEIRO DE SENSORIAMENTO REMOTO, 15., 2011, Curitiba. Anais... São José dos Campos: INPE, 2011. p. 9166-9172 SBSR 2011.| Biblioteca(s): Embrapa Cerrados. |
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| 3. | | LIMA, L. A. DE S.; MARTINS, E. de S.; BRAGA, A. R. dos S.; CASTRO, K. B. DE; SOUZA, V. V. DE. Mapeamento geomorfológico da bacia hidrográfica do Alto Rio Preto, GO, DF E MG, a partir do processamento digital de imagem SRTM. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 111-112 (Embrapa Cerrados. Documentos, 243).| Biblioteca(s): Embrapa Cerrados. |
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| 5. | | MARTINS, E. de S.; CARVALHO JÚNIOR, O. A. de; SOUZA, V. V. de; COUTO JÚNIOR, A. F.; OLIVEIRA, S. N. de; GOMES, R. A. T.; REATTO, A. Levantamento de reconhecimento de média intensidade dos solos do Parque Nacional da Serra dos Órgãos - Parnaso, Rio de Janeiro, escala 1:100.000. Planaltina, DF: Embrapa Cerrados, 2008. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 209).| Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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| 6. | | SOUZA, V. V.; NORONHA, S.; FONTES, E. M. G.; SUJII, E. R.; BARROSO, P.; PAIS, J. S. O.; CARDOSO, C. F.; CIAMPI, A.; PIRES, C. S. S. Distribuição geográfica, conservação e uso de variedades silvestres de Gossypium SPP. no Distrito Federal E Noroeste de Minas Gerais. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 120.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | LIMA, L. A. de S.; MARTINS, E. de S.; REATTO, A.; CASTRO, K. B. de; SOUZA, V. V. de; CARVALHO JUNIOR, O. A. de. Compartimentação geomorfológica e suas relações com solos da Bacia do Alto Rio Preto, GO. Planaltina, DF: Embrapa Cerrados, 2009. 24 p. (Embrapa Cerrados. Boletim de pesquisa e desenvolvimento, 255).| Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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| 8. | | SOUZA, V. V. DE; COUTO JUNIOR, A. F.; MARTINS, E. de S.; BRAGA, A. R. dos S.; CARVALHO JUNIOR, O. A. DE. Soil-vegetation relationships in savanna landscapes of the Serra da Canastra Plateau, Minas Gerais, Brazil. WORLD CONGRESS OF SOIL SCIENCE, 19., 2010, Brisbane. Soil solutions for a changing world. Brisbane: International Union of Soil Science: ASSSI, 2010. 1 CD-ROM. GIKES, R. J.; PRAKONGKEP, N. (Ed.).| Biblioteca(s): Embrapa Cerrados. |
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| 9. | | MARTINS, E. de S.; CARVALHO JÚNIOR, O. A. de; SOUZA, V. V. de; COUTO JÚNIOR, A. F.; OLIVEIRA, S. N. de; GOMES, R. A. T.; REATTO, A. Relação solo-relevo em vertentes assimétricas no Parque Nacional da Serra dos Órgãos, RJ. Revista Brasileira de Geomorfologia, v. 8, n. 1, p. 45-62, 2007.| Biblioteca(s): Embrapa Cerrados. |
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| 10. | | CASTRO, K. B. de; MARTINS, E. de S.; BRAGA, A. R. dos S.; LIMA, L. A. de S.; RODRIGUES, L. N.; CARVALHO JUNIOR, O. A.; BORGES, M. E. S.; SOUZA, V. V. de; GOMES, M. P. Compartimentação geomorfológica da bacia hidrográfica do rio Buriti Vermelho, Distrito Federal, DF. Planaltina, DF : Embrapa Cerrados, 2009. 23 23 p. (Embrapa Cerrados. Boletim de Pesquisa, 244)| Biblioteca(s): Embrapa Cerrados. |
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| 11. | | CASTRO, K. B. de; MARTINS, E. de S.; REATTO, A.; LIMA, L. A. de S.; RODRIGUES, L. N.; CARVALHO JUNIOR, O. A. de; BORGES, M. E. S.; SOUZA, V. V. de; GOMES, M. P. Compartimentação geomorfológica da Bacia Hidrográfica do Rio Buriti Vermelho, Distrito Federal, DF. Planaltina, DF: Embrapa Cerrados, 2009. 23 p. (Embrapa Cerrados. Boletim de pesquisa e desenvolvimento, 244).| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | SOUZA, V. V. de; MARTINS, E. de S.; BRAGA, A. R. dos S.; COUTO JÚNIOR, A. F.; CARVALHO JÚNIOR, O. A.; SANTOS, W. C. dos; LIMA, L. A. S.; CASTRO, K. B.; XAVIER, D.; PASSO, D. P.; GOMES, R. A. T. Caracterização de uma topossequência no Parque Nacional da Serra da Canastra, MG. Planaltina, DF: Embrapa Cerrados, 2010. 68 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 277).| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 12 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
28/06/2021 |
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Data da última atualização: |
28/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DeBLASIO, S. L.; WILSON, J. R.; TAMBORINDEGUY, C.; JOHNSON, R. S.; PINHEIRO, P. V.; MacCOSS, M. J.; GRAY, S. M.; HECK, M. |
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Afiliação: |
STACY L. DEBLASIO; JENNIFER R. WILSON; CECILIA TAMBORINDEGUY; RICHARD S. JOHNSON; PATRICIA VALLE PINHEIRO, CNPAF; MICHAEL J. MACCOSS; STEWART M. GRAY; MICHELLE HECK. |
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Título: |
Affinity purification - mass spectrometry identifies a novel interaction between a polerovirus and a conserved innate immunity aphid protein that regulates transmission efficiency. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Proteome Research, v. 20, n. 6, p. 3365-3387, June 2021. |
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Série: |
1535-3907 |
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DOI: |
https://doi.org/10.1021/acs.jproteome.1c00313 |
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Idioma: |
Inglês |
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Conteúdo: |
The vast majority of plant viruses are transmitted by insect vectors, with many crucial aspects of the transmission process being mediated by key protein?protein interactions. Still, very few vector proteins interacting with viruses have been identified and functionally characterized. Potato leafroll virus (PLRV) is transmitted most efficiently by Myzus persicae, the green peach aphid, in a circulative, non-propagative manner. Using affinity purification coupled to high-resolution mass spectrometry (AP-MS), we identified 11 proteins from M. persicaedisplaying a high probability of interaction with PLRV and an additional 23 vector proteins with medium confidence interaction scores. Three of these aphid proteins were confirmed to directly interact with the structural proteins of PLRV and other luteovirid species via yeast two-hybrid. Immunolocalization of one of these direct PLRV-interacting proteins, an orthologue of the human innate immunity protein complement component 1 Q subcomponent-binding protein (C1QBP), shows that MpC1QBP partially co-localizes with PLRV in cytoplasmic puncta and along the periphery of aphid gut epithelial cells. Artificial diet delivery to aphids of a chemical inhibitor of C1QBP leads to increased PLRV acquisition by aphids and subsequently increased titer in inoculated plants, supporting a role for C1QBP in the acquisition and transmission efficiency of PLRV by M. persicae. This study presents the first use of AP-MS for the in vivo isolation of a functionally relevant insect vector-virus protein complex. MS data are available from ProteomeXchange.org using the project identifier PXD022167. MenosThe vast majority of plant viruses are transmitted by insect vectors, with many crucial aspects of the transmission process being mediated by key protein?protein interactions. Still, very few vector proteins interacting with viruses have been identified and functionally characterized. Potato leafroll virus (PLRV) is transmitted most efficiently by Myzus persicae, the green peach aphid, in a circulative, non-propagative manner. Using affinity purification coupled to high-resolution mass spectrometry (AP-MS), we identified 11 proteins from M. persicaedisplaying a high probability of interaction with PLRV and an additional 23 vector proteins with medium confidence interaction scores. Three of these aphid proteins were confirmed to directly interact with the structural proteins of PLRV and other luteovirid species via yeast two-hybrid. Immunolocalization of one of these direct PLRV-interacting proteins, an orthologue of the human innate immunity protein complement component 1 Q subcomponent-binding protein (C1QBP), shows that MpC1QBP partially co-localizes with PLRV in cytoplasmic puncta and along the periphery of aphid gut epithelial cells. Artificial diet delivery to aphids of a chemical inhibitor of C1QBP leads to increased PLRV acquisition by aphids and subsequently increased titer in inoculated plants, supporting a role for C1QBP in the acquisition and transmission efficiency of PLRV by M. persicae. This study presents the first use of AP-MS for the in vivo isolation of a f... Mostrar Tudo |
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Palavras-Chave: |
Affinity purification; Aphids; Non-model system; Vector-pathogen interactions. |
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Thesaurus NAL: |
Luteoviridae; Mass spectrometry; Myzus; Phloem; Polerovirus; Potato leafroll virus; Purification methods. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02793naa a2200361 a 4500
001 2132597
005 2021-06-28
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1021/acs.jproteome.1c00313$2DOI
100 1 $aDeBLASIO, S. L.
245 $aAffinity purification - mass spectrometry identifies a novel interaction between a polerovirus and a conserved innate immunity aphid protein that regulates transmission efficiency.$h[electronic resource]
260 $c2021
490 $a1535-3907
520 $aThe vast majority of plant viruses are transmitted by insect vectors, with many crucial aspects of the transmission process being mediated by key protein?protein interactions. Still, very few vector proteins interacting with viruses have been identified and functionally characterized. Potato leafroll virus (PLRV) is transmitted most efficiently by Myzus persicae, the green peach aphid, in a circulative, non-propagative manner. Using affinity purification coupled to high-resolution mass spectrometry (AP-MS), we identified 11 proteins from M. persicaedisplaying a high probability of interaction with PLRV and an additional 23 vector proteins with medium confidence interaction scores. Three of these aphid proteins were confirmed to directly interact with the structural proteins of PLRV and other luteovirid species via yeast two-hybrid. Immunolocalization of one of these direct PLRV-interacting proteins, an orthologue of the human innate immunity protein complement component 1 Q subcomponent-binding protein (C1QBP), shows that MpC1QBP partially co-localizes with PLRV in cytoplasmic puncta and along the periphery of aphid gut epithelial cells. Artificial diet delivery to aphids of a chemical inhibitor of C1QBP leads to increased PLRV acquisition by aphids and subsequently increased titer in inoculated plants, supporting a role for C1QBP in the acquisition and transmission efficiency of PLRV by M. persicae. This study presents the first use of AP-MS for the in vivo isolation of a functionally relevant insect vector-virus protein complex. MS data are available from ProteomeXchange.org using the project identifier PXD022167.
650 $aLuteoviridae
650 $aMass spectrometry
650 $aMyzus
650 $aPhloem
650 $aPolerovirus
650 $aPotato leafroll virus
650 $aPurification methods
653 $aAffinity purification
653 $aAphids
653 $aNon-model system
653 $aVector-pathogen interactions
700 1 $aWILSON, J. R.
700 1 $aTAMBORINDEGUY, C.
700 1 $aJOHNSON, R. S.
700 1 $aPINHEIRO, P. V.
700 1 $aMacCOSS, M. J.
700 1 $aGRAY, S. M.
700 1 $aHECK, M.
773 $tJournal of Proteome Research$gv. 20, n. 6, p. 3365-3387, June 2021.
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