02793naa a2200361 a 450000100080000000500110000800800410001902400550006010000200011524502080013526000090034349000140035252016480036665000170201465000220203165000100205365000110206365000160207465000260209065000250211665300260214165300110216765300210217865300330219970000180223270000220225070000190227270000200229170000190231170000160233070000130234677300720235921325972021-06-28       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1021/acs.jproteome.1c003132DOI1 aDeBLASIO, S. L.  aAffinity purification - mass spectrometry identifies a novel interaction between a polerovirus and a conserved innate immunity aphid protein that regulates transmission efficiency.h[electronic resource]  c2021  a1535-3907  aThe vast majority of plant viruses are transmitted by insect vectors, with many crucial aspects of the transmission process being mediated by key protein?protein interactions. Still, very few vector proteins interacting with viruses have been identified and functionally characterized. Potato leafroll virus (PLRV) is transmitted most efficiently by Myzus persicae, the green peach aphid, in a circulative, non-propagative manner. Using affinity purification coupled to high-resolution mass spectrometry (AP-MS), we identified 11 proteins from M. persicaedisplaying a high probability of interaction with PLRV and an additional 23 vector proteins with medium confidence interaction scores. Three of these aphid proteins were confirmed to directly interact with the structural proteins of PLRV and other luteovirid species via yeast two-hybrid. Immunolocalization of one of these direct PLRV-interacting proteins, an orthologue of the human innate immunity protein complement component 1 Q subcomponent-binding protein (C1QBP), shows that MpC1QBP partially co-localizes with PLRV in cytoplasmic puncta and along the periphery of aphid gut epithelial cells. Artificial diet delivery to aphids of a chemical inhibitor of C1QBP leads to increased PLRV acquisition by aphids and subsequently increased titer in inoculated plants, supporting a role for C1QBP in the acquisition and transmission efficiency of PLRV by M. persicae. This study presents the first use of AP-MS for the in vivo isolation of a functionally relevant insect vector-virus protein complex. MS data are available from ProteomeXchange.org using the project identifier PXD022167.  aLuteoviridae  aMass spectrometry  aMyzus  aPhloem  aPolerovirus  aPotato leafroll virus  aPurification methods  aAffinity purification  aAphids  aNon-model system  aVector-pathogen interactions1 aWILSON, J. R.1 aTAMBORINDEGUY, C.1 aJOHNSON, R. S.1 aPINHEIRO, P. V.1 aMacCOSS, M. J.1 aGRAY, S. M.1 aHECK, M.  tJournal of Proteome Researchgv. 20, n. 6, p. 3365-3387, June 2021.