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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
04/08/2010 |
Data da última atualização: |
11/05/2011 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PAUVOLID-CORRÊA, A.; TAVARES, F. N.; ALENCAR, J.; SILVA, J. dos S.; MURTA, M.; SERRA-FREIRE, N. M.; PELLEGRIN, A. O.; GIL-SANTANA, H.; GUIMARÃES, A. E.; SILVA, E. E. da. |
Afiliação: |
ALEX PAUVOLID-CORRÊA, FUNDAÇÃO OSWALDO CRUZ; FERNANDO NETO TAVARES, FUNDAÇÃO OSWALDO CRUZ; JERONIMO ALENCAR, FUNDAÇÃO OSWALDO CRUZ; JULIA DOS SANTOS SILVA, FUNDAÇÃO OSWALDO CRUZ; MICHELE MURTA, FUNDAÇÃO OSWALDO CRUZ; NICOLAU MAUÉS SERRA-FREIRE, FUNDAÇÃO OSWALDO CRUZ; AIESCA OLIVEIRA PELLEGRIN, CPAP; HÉLCIO GIL-SANTANA, FUNDAÇÃO OSWALDO CRUZ; ANTHONY ÉRICO GUIMARÃES, FUNDAÇÃO OSWALDO CRUZ; EDSON ELIAS DA SILVA. |
Título: |
Preliminary investigation of culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Revista do Instituto de Medicina Tropical de São Paulo, v. 52, n. 1, p. 17-23, Jan/ fev. 2010. |
DOI: |
10.1590/S0036-46652010000100004 |
Idioma: |
Inglês |
Conteúdo: |
In view of the high circulation of migratory birds and the environmental and climatic conditions which favor the proliferation of arthropods, the Brazilian Pantanal is susceptible to circulation of arboviruses. However, the amount of data concerning arbovirus
vectors in this area is scarce; therefore the aim of this study was to conduct a preliminary investigation of Culicidae species in the
Nhecolândia Sub-region of South Pantanal, Brazil and their potential importance in the arbovirus transmission. A total of 3684 specimens of mosquitoes were captured, 1689 of which caught in the rainy season of 2007, were divided into 78 pools and submitted
to viral isolation, Semi-Nested RT-PCR and Nested RT-PCR, with a view to identifying the most important arboviruses in Brazil. Simultaneously, 70 specimens of ticks found blood-feeding on horses were also submitted to the same virological assays. No virus was isolated and viral nucleic-acid detection by RT-PCR was also negative. Nevertheless, a total of 22 Culicidae species were identified, ten of which had previously been reported as vectors of important arboviruses. The diversity of species found blood-feeding on human and horse hosts together with the arboviruses circulation previously reported suggest that the Nhecolândia Sub-region of South Pantanal is an important area for arbovirus surveillance in Brazil. |
Palavras-Chave: |
Arbovirus; Nhecolândia. |
Thesaurus Nal: |
Culicidae; Pantanal; ticks. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02300naa a2200301 a 4500 001 1859207 005 2011-05-11 008 2010 bl --- 0-- u #d 024 7 $a10.1590/S0036-46652010000100004$2DOI 100 1 $aPAUVOLID-CORRÊA, A. 245 $aPreliminary investigation of culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission. 260 $c2010 520 $aIn view of the high circulation of migratory birds and the environmental and climatic conditions which favor the proliferation of arthropods, the Brazilian Pantanal is susceptible to circulation of arboviruses. However, the amount of data concerning arbovirus vectors in this area is scarce; therefore the aim of this study was to conduct a preliminary investigation of Culicidae species in the Nhecolândia Sub-region of South Pantanal, Brazil and their potential importance in the arbovirus transmission. A total of 3684 specimens of mosquitoes were captured, 1689 of which caught in the rainy season of 2007, were divided into 78 pools and submitted to viral isolation, Semi-Nested RT-PCR and Nested RT-PCR, with a view to identifying the most important arboviruses in Brazil. Simultaneously, 70 specimens of ticks found blood-feeding on horses were also submitted to the same virological assays. No virus was isolated and viral nucleic-acid detection by RT-PCR was also negative. Nevertheless, a total of 22 Culicidae species were identified, ten of which had previously been reported as vectors of important arboviruses. The diversity of species found blood-feeding on human and horse hosts together with the arboviruses circulation previously reported suggest that the Nhecolândia Sub-region of South Pantanal is an important area for arbovirus surveillance in Brazil. 650 $aCulicidae 650 $aPantanal 650 $aticks 653 $aArbovirus 653 $aNhecolândia 700 1 $aTAVARES, F. N. 700 1 $aALENCAR, J. 700 1 $aSILVA, J. dos S. 700 1 $aMURTA, M. 700 1 $aSERRA-FREIRE, N. M. 700 1 $aPELLEGRIN, A. O. 700 1 $aGIL-SANTANA, H. 700 1 $aGUIMARÃES, A. E. 700 1 $aSILVA, E. E. da 773 $tRevista do Instituto de Medicina Tropical de São Paulo$gv. 52, n. 1, p. 17-23, Jan/ fev. 2010.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
24/02/2017 |
Data da última atualização: |
27/06/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
PAIXÃO, F. R. S.; MUNIZ, E. R.; BARRETO, L. P.; BERNARDO, C. C.; MASCARIN, G. M.; LUZ, C.; FERNANDES, E. K. K. |
Afiliação: |
UFG; UFG; UFG; UFG; GABRIEL MOURA MASCARIN, CNPAF; UFG; UFG. |
Título: |
Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Biocontrol Science and Technology, v. 27, n. 3, p. 324-337, 2017. |
DOI: |
10.1080/09583157.2017.1281380 |
Idioma: |
Inglês |
Conteúdo: |
The thermotolerance of oil-based conidial formulations of Metarhizium anisopliae s.l. (IP 46) and Metarhizium robertsii (ARSEF 2575) were investigated. Conidia of IP 46 or ARSEF 2575 were suspended in different adjuvants and exposed to 45±0.2°C for 4, 6, 8 or 24 h; their viability was then assessed after 48 h incubation at 27 ± 1°C. Conidia heated in pure mineral or vegetable oil exhibited mean relative viability exceeding 70% after 8 h of heat exposure, whereas low germination (20%) was observed when conidia were heated in water (Tween 80® 0.01%), carboxymethyl cellulose gel or emulsifiable oils (Graxol® or Assist®) and exposed to heat for 6 or 8 h. In addition, conidia of IP 46 suspended in either pure mineral or canola oil and exposed to heat for 48 h had moderate viability, 57% or 41%, respectively. Unstable oil-in-water emulsions showed a higher percentage of conidia incorporated into oil micellae, while the stable emulsions had higher percentage of conidia outside the oil micellae. The thermotolerance of conidia formulated in stable emulsions, however, did not differ from that of conidia formulated in unstable emulsions. The present study highlights possibilities to alleviate the deleterious effects of heat stress towards Metarhizium spp. conidia applied for controlling arthropod pests and vectors through oil-based formulations. |
Palavras-Chave: |
Conidial thermotolerance; Metharizium. |
Thesaurus NAL: |
Adjuvants; Emulsions; Entomopathogenic fungi. |
Categoria do assunto: |
O Insetos e Entomologia |
Marc: |
LEADER 02195naa a2200265 a 4500 001 2065546 005 2017-06-27 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1080/09583157.2017.1281380$2DOI 100 1 $aPAIXÃO, F. R. S. 245 $aIncreased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii.$h[electronic resource] 260 $c2017 520 $aThe thermotolerance of oil-based conidial formulations of Metarhizium anisopliae s.l. (IP 46) and Metarhizium robertsii (ARSEF 2575) were investigated. Conidia of IP 46 or ARSEF 2575 were suspended in different adjuvants and exposed to 45±0.2°C for 4, 6, 8 or 24 h; their viability was then assessed after 48 h incubation at 27 ± 1°C. Conidia heated in pure mineral or vegetable oil exhibited mean relative viability exceeding 70% after 8 h of heat exposure, whereas low germination (20%) was observed when conidia were heated in water (Tween 80® 0.01%), carboxymethyl cellulose gel or emulsifiable oils (Graxol® or Assist®) and exposed to heat for 6 or 8 h. In addition, conidia of IP 46 suspended in either pure mineral or canola oil and exposed to heat for 48 h had moderate viability, 57% or 41%, respectively. Unstable oil-in-water emulsions showed a higher percentage of conidia incorporated into oil micellae, while the stable emulsions had higher percentage of conidia outside the oil micellae. The thermotolerance of conidia formulated in stable emulsions, however, did not differ from that of conidia formulated in unstable emulsions. The present study highlights possibilities to alleviate the deleterious effects of heat stress towards Metarhizium spp. conidia applied for controlling arthropod pests and vectors through oil-based formulations. 650 $aAdjuvants 650 $aEmulsions 650 $aEntomopathogenic fungi 653 $aConidial thermotolerance 653 $aMetharizium 700 1 $aMUNIZ, E. R. 700 1 $aBARRETO, L. P. 700 1 $aBERNARDO, C. C. 700 1 $aMASCARIN, G. M. 700 1 $aLUZ, C. 700 1 $aFERNANDES, E. K. K. 773 $tBiocontrol Science and Technology$gv. 27, n. 3, p. 324-337, 2017.
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Embrapa Arroz e Feijão (CNPAF) |
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