02300naa a2200301 a 450000100080000000500110000800800410001902400410006010000250010124501350012626000090026152013830027065000140165365000130166765000100168065300140169065300170170470000190172170000160174070000210175670000140177770000240179170000210181570000200183670000220185670000200187877301000189818592072011-05-11       2010    bl ---       0--  u    #d7 a10.1590/S0036-466520100001000042DOI1 aPAUVOLID-CORRÊA, A.  aPreliminary investigation of culicidae species in South Pantanal, Brazil and their potential importance in arbovirus transmission.  c2010  aIn view of the high circulation of migratory birds and the environmental and climatic conditions which favor the proliferation of arthropods, the Brazilian Pantanal is susceptible to circulation of arboviruses. However, the amount of data concerning arbovirus  vectors in this area is scarce; therefore the aim of this study was to conduct a preliminary investigation of Culicidae species in the  Nhecolândia Sub-region of South Pantanal, Brazil and their potential importance in the arbovirus transmission. A total of 3684 specimens of mosquitoes were captured, 1689 of which caught in the rainy season of 2007, were divided into 78 pools and submitted  to viral isolation, Semi-Nested RT-PCR and Nested RT-PCR, with a view to identifying the most important arboviruses in Brazil. Simultaneously, 70 specimens of ticks found blood-feeding on horses were also submitted to the same virological assays. No virus was isolated and viral nucleic-acid detection by RT-PCR was also negative. Nevertheless, a total of 22 Culicidae species were identified, ten of which had previously been reported as vectors of important arboviruses. The diversity of species found blood-feeding on human and horse hosts together with the arboviruses circulation previously reported suggest that the Nhecolândia Sub-region of South Pantanal is an important area for arbovirus surveillance in Brazil.  aCulicidae  aPantanal  aticks  aArbovirus  aNhecolândia1 aTAVARES, F. N.1 aALENCAR, J.1 aSILVA, J. dos S.1 aMURTA, M.1 aSERRA-FREIRE, N. M.1 aPELLEGRIN, A. O.1 aGIL-SANTANA, H.1 aGUIMARÃES, A. E.1 aSILVA, E. E. da  tRevista do Instituto de Medicina Tropical de São Paulogv. 52, n. 1, p. 17-23, Jan/ fev. 2010.