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| Registros recuperados : 290 | |
| Registros recuperados : 290 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/09/2023 |
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Data da última atualização: |
06/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA, G. H. da; FRANQUI, L. S.; FARIAS, M. A. de; CASTRO, V. L. S. S. de; BYRNE, H. J.; MARTINEZ, D. S.; MONTEIRO, R. T.; CASEY, A. |
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Afiliação: |
GABRIELA H. DA SILVA, CENTRO NACIONAL DE PESQUISA EM ENERGIA E MATERIAIS; LIDIANE SILVA FRANQUI, CENTRO NACIONAL DE PESQUISA EM ENERGIA E MATERIAIS; MARCELO A. DE FARIAS, CENTRO NACIONAL DE PESQUISA EM ENERGIA E MATERIAIS; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; HUGH J. BYRNE, FOCAS RESEARCH INSTITUTE; DIEGO S.T. MARTINEZ, UNIVERSIDADE DE SÃO PAULO; REGINA T.R. MONTEIRO, UNIVERSIDADE DE SÃO PAULO; ALAN CASEY, FOCAS RESEARCH INSTITUTE. |
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Título: |
TiO2-MWCNT nanohybrid: Cytotoxicity, protein corona formation and cellular internalisation in RTG-2 fish cell line |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Aquatic Toxicology, v.257, article106434, 2023. |
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ISSN: |
0166-445X |
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DOI: |
https://doi.org/10.1016/j.aquatox.2023.106434 |
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Idioma: |
Português |
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Conteúdo: |
Abstract: Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO2-MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the toxicological effects of TiO2-MWCNT towards safe and sustainable development of nanohybrids. In this work, we studied the cytotoxicity, protein corona formation and cellular internalisation of TiO2-MWCNT on fibroblasts derived from gonadal rainbow trout tissue (RTG-2) for the first time. This nanohydrid did not show any toxicity effect on RTG-2 cells up to 100 mg L-1 after 24 h of exposure as monitored by alamar blue, neutral red and trypan blue assays (in presence or absence of foetal bovine serum, FBS). Futhermore, cryo-transmission electron microscopy analysis demonstrated that TiO2 particles is attached on nanotube surface after FBS-protein corona formation in cell culture medium. Raman spectroscopy imaging showed that TiO2-MWCNT can be internalised by RTG-2 cells. This work is a novel contribution towards better understanding the nanobiointeractions of nanohydrids linked to their in vitro effects on fish cells in aquatic nanoecotoxicology. |
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Palavras-Chave: |
Nanosafety; Protein corona. |
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Thesagro: |
Composto de Carbono; Peixe de Água Doce; Toxidez. |
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Thesaurus NAL: |
Carbon nanotubes; Ecotoxicology; Nanoparticles; Raman spectroscopy; Titanium dioxide. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02309naa a2200349 a 4500
001 2156365
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008 2023 bl uuuu u00u1 u #d
022 $a0166-445X
024 7 $ahttps://doi.org/10.1016/j.aquatox.2023.106434$2DOI
100 1 $aSILVA, G. H. da
245 $aTiO2-MWCNT nanohybrid$bCytotoxicity, protein corona formation and cellular internalisation in RTG-2 fish cell line$h[electronic resource]
260 $c2023
520 $aAbstract: Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO2-MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the toxicological effects of TiO2-MWCNT towards safe and sustainable development of nanohybrids. In this work, we studied the cytotoxicity, protein corona formation and cellular internalisation of TiO2-MWCNT on fibroblasts derived from gonadal rainbow trout tissue (RTG-2) for the first time. This nanohydrid did not show any toxicity effect on RTG-2 cells up to 100 mg L-1 after 24 h of exposure as monitored by alamar blue, neutral red and trypan blue assays (in presence or absence of foetal bovine serum, FBS). Futhermore, cryo-transmission electron microscopy analysis demonstrated that TiO2 particles is attached on nanotube surface after FBS-protein corona formation in cell culture medium. Raman spectroscopy imaging showed that TiO2-MWCNT can be internalised by RTG-2 cells. This work is a novel contribution towards better understanding the nanobiointeractions of nanohydrids linked to their in vitro effects on fish cells in aquatic nanoecotoxicology.
650 $aCarbon nanotubes
650 $aEcotoxicology
650 $aNanoparticles
650 $aRaman spectroscopy
650 $aTitanium dioxide
650 $aComposto de Carbono
650 $aPeixe de Água Doce
650 $aToxidez
653 $aNanosafety
653 $aProtein corona
700 1 $aFRANQUI, L. S.
700 1 $aFARIAS, M. A. de
700 1 $aCASTRO, V. L. S. S. de
700 1 $aBYRNE, H. J.
700 1 $aMARTINEZ, D. S.
700 1 $aMONTEIRO, R. T.
700 1 $aCASEY, A.
773 $tAquatic Toxicology$gv.257, article106434, 2023.
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