02310naa a2200349 a 450000100080000000500110000800800410001902200140006002400550007410000200012924501430014926000090029252012740030165000210157565000180159665000180161465000230163265000210165565000240167665000240170065000120172465300150173665300190175170000190177070000210178970000270181070000170183770000200185470000200187470000140189477300520190821563652023-09-06       2023    bl uuuu     u00u1 u    #d  a0166-445X7 ahttps://doi.org/10.1016/j.aquatox.2023.1064342DOI1 aSILVA, G. H. da  aTiO2-MWCNT nanohybridbCytotoxicity, protein corona formation and cellular internalisation in RTG-2 fish cell line.h[electronic resource]  c2023  aAbstract: Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO2-MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the toxicological effects of TiO2-MWCNT towards safe and sustainable development of nanohybrids. In this work, we studied the cytotoxicity, protein corona formation and cellular internalisation of TiO2-MWCNT on fibroblasts derived from gonadal rainbow trout tissue (RTG-2) for the first time. This nanohydrid did not show any toxicity effect on RTG-2 cells up to 100 mg L-1 after 24 h of exposure as monitored by alamar blue, neutral red and trypan blue assays (in presence or absence of foetal bovine serum, FBS). Futhermore, cryo-transmission electron microscopy analysis demonstrated that TiO2 particles is attached on nanotube surface after FBS-protein corona formation in cell culture medium. Raman spectroscopy imaging showed that TiO2-MWCNT can be internalised by RTG-2 cells. This work is a novel contribution towards better understanding the nanobiointeractions of nanohydrids linked to their in vitro effects on fish cells in aquatic nanoecotoxicology.  aCarbon nanotubes  aEcotoxicology  aNanoparticles  aRaman spectroscopy  aTitanium dioxide  aComposto de Carbono  aPeixe de Água Doce  aToxidez  aNanosafety  aProtein corona1 aFRANQUI, L. S.1 aFARIAS, M. A. de1 aCASTRO, V. L. S. S. de1 aBYRNE, H. J.1 aMARTINEZ, D. S.1 aMONTEIRO, R. T.1 aCASEY, A.  tAquatic Toxicologygv.257, article106434, 2023.