| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Amapá. Para informações adicionais entre em contato com cpafap.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Amapá. |
|
Data corrente: |
29/11/2022 |
|
Data da última atualização: |
29/11/2022 |
|
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
|
Autoria: |
BORGES, W. L.; COSTA, J. S. de S.; MELO, L. P. de; ARAUJO, N. M.; SARAIVA, O. do N.; PEDRADA, A. K. L.; ARAÚJO, D. S. de. |
|
Afiliação: |
WARDSSON LUSTRINO BORGES, CPAF-AP; JANAYNA SANTOS DE SOUSA COSTA, INSTITUTO DE DESENVOLVIMENTO RURAL DO AMAPÁ; LARISSA PINHEIRO DE MELO, INSTITUTO FEDERAL DO AMAPÁ; NADIANE MUNHOZ ARAUJO, UNIVERSIDADE DO ESTADO DO AMAPÁ; OLIVAN DO NASCIMENTO SARAIVA, AGÊNCIA DE DEFESA E INSPEÇÃO AGROPECUÁRIA DO AMAPÁ; ANA KAROLINA LIMA PEDRADA, INSTITUTO FEDERAL DO AMAPÁ; DÉBORA SILVA DE ARAÚJO, UNIVERSIDADE FEDERAL DO AMAPÁ. |
|
Título: |
Desenvolvimento e agricultura: o estado do Amapá. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
In: GOMES, A. F.; QUARESMA, P.; GIRALDI, P.; SANTOS, V. F. dos; PORTO, J. (org.). Mestrado em desenvolvimento regional: 15 anos, na busca de sinergias, possibilidades e expectativas de desenvolvimento. Maringá: Uniedusul, 2022. |
|
Páginas: |
p. 25-37. |
|
DOI: |
10.51324/80277988.2 |
|
Idioma: |
Português |
|
Notas: |
Debates efetuados no III Simpósio de Pós-graduação em Desenvolvimento Regional, 2021. |
|
Palavras-Chave: |
Desenvolvimento regional. |
|
Thesagro: |
Agricultura; Desenvolvimento Agrícola. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00990naa a2200253 a 4500
001 2148946
005 2022-11-29
008 2022 bl uuuu u00u1 u #d
024 7 $a10.51324/80277988.2$2DOI
100 1 $aBORGES, W. L.
245 $aDesenvolvimento e agricultura$bo estado do Amapá.$h[electronic resource]
260 $c2022
300 $ap. 25-37.
500 $aDebates efetuados no III Simpósio de Pós-graduação em Desenvolvimento Regional, 2021.
650 $aAgricultura
650 $aDesenvolvimento Agrícola
653 $aDesenvolvimento regional
700 1 $aCOSTA, J. S. de S.
700 1 $aMELO, L. P. de
700 1 $aARAUJO, N. M.
700 1 $aSARAIVA, O. do N.
700 1 $aPEDRADA, A. K. L.
700 1 $aARAÚJO, D. S. de
773 $tIn: GOMES, A. F.; QUARESMA, P.; GIRALDI, P.; SANTOS, V. F. dos; PORTO, J. (org.). Mestrado em desenvolvimento regional: 15 anos, na busca de sinergias, possibilidades e expectativas de desenvolvimento. Maringá: Uniedusul, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amapá (CPAF-AP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Cerrados. |
|
Data corrente: |
19/09/2022 |
|
Data da última atualização: |
19/09/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
MATTOS, L. M.; REIS, F. C.; RACANICCI, A. M. C.; PIVATO, I.; TONELLI, G. S. S. S.; BÁO, S. N.; MARTINS, C. F. |
|
Afiliação: |
LUCIANA MIRANDA MATTOS; FILIPE CARNEIRO REIS; ALINE M.C. RACANICCI; IVO PIVATO; GUILHERME S.S.S. TONELLI; SÔNIA NAIR BÁO; CARLOS FREDERICO MARTINS, CPAC. |
|
Título: |
Freezing for the Future: obtaining fibroblast samples from deceased wild mammals for the Brazilian Cerrado Germplasm Bank. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Biopreservation and Biobanking, ahead of print, 2022. |
|
DOI: |
DOI: 10.1089/bio.2022.0039 |
|
Idioma: |
Inglês |
|
Conteúdo: |
We isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank containing somatic cells and gametes of wild mammals of the Cerrado biome of Brazil. This material will be used for future characterization of the species and multiplication by means of nuclear transfer cloning. MenosWe isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank contain... Mostrar Tudo |
|
Thesagro: |
Animal Selvagem; Banco de Germoplasma; Biotecnologia; Criopreservação. |
|
Thesaurus NAL: |
Biotechnology; Cryopreservation; Germplasm; Wild animals. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02613naa a2200301 a 4500
001 2146609
005 2022-09-19
008 2022 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1089/bio.2022.0039$2DOI
100 1 $aMATTOS, L. M.
245 $aFreezing for the Future$bobtaining fibroblast samples from deceased wild mammals for the Brazilian Cerrado Germplasm Bank.$h[electronic resource]
260 $c2022
520 $aWe isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank containing somatic cells and gametes of wild mammals of the Cerrado biome of Brazil. This material will be used for future characterization of the species and multiplication by means of nuclear transfer cloning.
650 $aBiotechnology
650 $aCryopreservation
650 $aGermplasm
650 $aWild animals
650 $aAnimal Selvagem
650 $aBanco de Germoplasma
650 $aBiotecnologia
650 $aCriopreservação
700 1 $aREIS, F. C.
700 1 $aRACANICCI, A. M. C.
700 1 $aPIVATO, I.
700 1 $aTONELLI, G. S. S. S.
700 1 $aBÁO, S. N.
700 1 $aMARTINS, C. F.
773 $tBiopreservation and Biobanking, ahead of print, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Cerrados (CPAC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
