02613naa a2200301 a 450000100080000000500110000800800410001902400360006010000180009624501500011426000090026452017100027365000180198365000210200165000140202265000170203665000200205365000250207365000180209865000220211670000160213870000240215470000150217870000250219370000160221870000190223477300580225321466092022-09-19       2022    bl uuuu     u00u1 u    #d7 aDOI: 10.1089/bio.2022.00392DOI1 aMATTOS, L. M.  aFreezing for the Futurebobtaining fibroblast samples from deceased wild mammals for the Brazilian Cerrado Germplasm Bank.h[electronic resource]  c2022  aWe isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p &lt 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank containing somatic cells and gametes of wild mammals of the Cerrado biome of Brazil. This material will be used for future characterization of the species and multiplication by means of nuclear transfer cloning.  aBiotechnology  aCryopreservation  aGermplasm  aWild animals  aAnimal Selvagem  aBanco de Germoplasma  aBiotecnologia  aCriopreservação1 aREIS, F. C.1 aRACANICCI, A. M. C.1 aPIVATO, I.1 aTONELLI, G. S. S. S.1 aBÁO, S. N.1 aMARTINS, C. F.  tBiopreservation and Biobanking, ahead of print, 2022.