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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
07/05/2020 |
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Data da última atualização: |
15/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, A. M. P.; SALLES, H. O.; OLIVEIRA, H. D. de; SOUZA, B. B. P. de; CARDOZO FILHO, J. de L.; SIFUENTES, D. N.; PRATES, M. V.; BLOCH JUNIOR, C.; BEMQUERER, M. P.; VASCONCELOS, A. S. do E. |
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Afiliação: |
ANA MARJORY PAIVA SOUSA, Rede Nordeste de Biotecnologia-RENORBIO, Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; HEVILA OLIVEIRA SALLES, CNPC; HERMOGENES DAVID DE OLIVEIRA, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; BEATRIZ BLENDA PINHEIRO DE SOUZA; JOSE DE LIMA CARDOZO FILHO; DANIEL NOGOCEKE SIFUENTES, Cenargen; MAURA VIANNA PRATES, Cenargen; CARLOS BLOCH JUNIOR, Cenargen; MARCELO PORTO BEMQUERER, Cenargen; ANTONIO SILVIO DO EGITO VASCONCELOS, CNPC. |
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Título: |
Mo-HLPs: new flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 217, article 103692, Apr. 2020. |
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DOI: |
https://doi.org/10.1016/j.jprot.2020.103692 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds. |
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Palavras-Chave: |
Cationic peptides; Flocculant activity; Hevein-like. |
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Thesagro: |
Moringa Oleifera. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02333naa a2200289 a 4500
001 2122135
005 2020-12-15
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jprot.2020.103692$2DOI
100 1 $aSOUSA, A. M. P.
245 $aMo-HLPs$bnew flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family.$h[electronic resource]
260 $c2020
520 $aAbstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI >10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds.
650 $aMoringa Oleifera
653 $aCationic peptides
653 $aFlocculant activity
653 $aHevein-like
700 1 $aSALLES, H. O.
700 1 $aOLIVEIRA, H. D. de
700 1 $aSOUZA, B. B. P. de
700 1 $aCARDOZO FILHO, J. de L.
700 1 $aSIFUENTES, D. N.
700 1 $aPRATES, M. V.
700 1 $aBLOCH JUNIOR, C.
700 1 $aBEMQUERER, M. P.
700 1 $aVASCONCELOS, A. S. do E.
773 $tJournal of Proteomics$gv. 217, article 103692, Apr. 2020.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Unidades Centrais. |
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Data corrente: |
29/11/1995 |
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Data da última atualização: |
14/05/1998 |
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Autoria: |
OLIVEIRA, G. P. de; MATSUMOTO, T. |
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Título: |
Prevalencia e intensidade de infeccao por Helmintos em bovinos da bacia leiteira de Sao Carlos, Sao Paulo. |
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Ano de publicação: |
1985 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.20, n.12, p.1415-1418, dez. 1985. |
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Idioma: |
Português |
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Conteúdo: |
Para estudo preliminar de helmintos de bovinos da bacia leiteira de Sao Carlos, SP, que abrange os municipios de Sao Carlos, Ribeirao Bonito e Dourado, foram necropsiados 74 animais mesticos Holandes-Zebu, na faixa etaria de 8 a 20 meses. Do material coletado do tubo digestivo, pulmoes e pancreas, foram observadas as especies de helmintos com as seguintes prevalencias e intensidades medias de infeccao: Haemonchus contortus, 100% e 988,06; Haemonchus similis 43,24% e 50,01; Trichostrongylus axei, 24,32% e 16,00; Cooperia punctata 100% e 3649,45; Cooperia pectinata 44,59% e 436,70; Bunostomum phlebotomum, 9,45% e 3,17; Oesophagostomum radiatum, 86,48% e 138,35; Trichuris discolor, 21,62% e 7,44; Agriostomum wryburgi, 2,7% e 0,22; Dictyocaulus viviparus 13,51% e 51,62; Eurytrema coelomatium, 14,86 e 27,67. |
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Palavras-Chave: |
Bacia leiteira de São Carlos; Bovinos; Bovinos mesticos leiteiros; Brasil; Crossbred dairy bovines; Infecção por helmintos. |
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Thesaurus NAL: |
Bovidae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/111177/1/16416-71438-1-SM.pdf
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Marc: |
LEADER 01522naa a2200217 a 4500
001 1104646
005 1998-05-14
008 1985 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, G. P. de
245 $aPrevalencia e intensidade de infeccao por Helmintos em bovinos da bacia leiteira de Sao Carlos, Sao Paulo.$h[electronic resource]
260 $c1985
520 $aPara estudo preliminar de helmintos de bovinos da bacia leiteira de Sao Carlos, SP, que abrange os municipios de Sao Carlos, Ribeirao Bonito e Dourado, foram necropsiados 74 animais mesticos Holandes-Zebu, na faixa etaria de 8 a 20 meses. Do material coletado do tubo digestivo, pulmoes e pancreas, foram observadas as especies de helmintos com as seguintes prevalencias e intensidades medias de infeccao: Haemonchus contortus, 100% e 988,06; Haemonchus similis 43,24% e 50,01; Trichostrongylus axei, 24,32% e 16,00; Cooperia punctata 100% e 3649,45; Cooperia pectinata 44,59% e 436,70; Bunostomum phlebotomum, 9,45% e 3,17; Oesophagostomum radiatum, 86,48% e 138,35; Trichuris discolor, 21,62% e 7,44; Agriostomum wryburgi, 2,7% e 0,22; Dictyocaulus viviparus 13,51% e 51,62; Eurytrema coelomatium, 14,86 e 27,67.
650 $aBovidae
653 $aBacia leiteira de São Carlos
653 $aBovinos
653 $aBovinos mesticos leiteiros
653 $aBrasil
653 $aCrossbred dairy bovines
653 $aInfecção por helmintos
700 1 $aMATSUMOTO, T.
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.20, n.12, p.1415-1418, dez. 1985.
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Embrapa Unidades Centrais (AI-SEDE) |
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