02333naa a2200289 a 450000100080000000500110000800800410001902400530006010000200011324501410013326000090027452014130028365000210169665300220171765300240173965300160176370000180177970000230179770000230182070000280184370000210187170000180189270000210191070000210193170000290195277300620198121221352020-12-15       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.jprot.2020.1036922DOI1 aSOUSA, A. M. P.  aMo-HLPsbnew flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family.h[electronic resource]  c2020  aAbstract: Cationic peptides found in Moringa oleifera seeds belong to different protein families and are described as the main flocculating agents of the species. In this study we report the identification and isolation of four new flocculant peptides, called Mo-HLPs 1?4, belonging to the family of hevein-like peptides, previously only known for their members' antimicrobial activity. Purification of the peptides followed two sequential membrane ultrafiltration steps and separation by reverse-phase liquid chromatography. Proteomic analyses showed that Mo-HLPs are extremely basic (pI &gt10) cysteine-rich molecules with molecular masses between 4.5 and 4.8 kDa and with a highly conserved chitin-binding domain. Searches in BLAST revealed high similarity of Mo-HLPs with hevein and other hevein-like peptides and 90% identity with morintides, which are members of the 8C-hevein-like subfamily found in M. oleifera leaves. Mo-HLPs microflocculation assays showed distinct coagulation/flocculation efficiencies, promoting turbidity reduction levels between 67 and 89% in synthetic turbid water. Activity variations were attributed to the substitution of some amino acids among the isoforms, which may have altered the final net charge of the molecules. The identification of Mo-HLPs represents the discovery of a new group of cationic peptides involved in the flocculation properties of M. oleifera seeds.  aMoringa Oleifera  aCationic peptides  aFlocculant activity  aHevein-like1 aSALLES, H. O.1 aOLIVEIRA, H. D. de1 aSOUZA, B. B. P. de1 aCARDOZO FILHO, J. de L.1 aSIFUENTES, D. N.1 aPRATES, M. V.1 aBLOCH JUNIOR, C.1 aBEMQUERER, M. P.1 aVASCONCELOS, A. S. do E.  tJournal of Proteomicsgv. 217, article 103692, Apr. 2020.