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| 2. |  | FRANCISCO, M. S.; MENEZES, E.; SANTOS, O. C. DA S.; LAPORT, M. S.; BRITO, M. A. V. P. e; MARVAL, M. G. DE. Caracterização de cepas de Staphylococcus Spp. produtora de biofilme isoladas a partir de leite de vacas com mastite subclínica. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçú. Resumos... Foz do Iguaçú: Sociedade Brasileira de Microbiologia, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
07/10/2021 |
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Data da última atualização: |
07/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
OLIVEIRA, T. C. de A.; BRITO, M. A. P. P.; MARVAL, M. G. de; VICENTINI, N. M.; LANGE, C. C. |
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Afiliação: |
THAIS CRISTINA DE ASSIS OLIVEIRA, Estudante, Universidade Federal de Juiz de Fora; MARIA APARECIDA VASCONCELOS PAIVA BRITO; MARCIA GIAMBIAGI DE MARVAL, Universidade Federal do Rio de Janeiro; NIVEA MARIA VICENTINI, CNPGL; CARLA CHRISTINE LANGE, CNPGL. |
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Título: |
Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Dairy Research, v. 88, p. 302-306, 2021. |
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DOI: |
https://doi.org/10.1017/ S0022029921000595 |
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Idioma: |
Inglês |
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Conteúdo: |
In this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil. |
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Palavras-Chave: |
Mastite; Staphylococci; Streptococci. |
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Thesagro: |
Bovino; Espectrometria; Estafilococo; Estreptococo; Infecção. |
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Thesaurus NAL: |
Bovine mastitis; Mass spectrometry. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02008naa a2200301 a 4500
001 2135151
005 2021-10-07
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/ S0022029921000595$2DOI
100 1 $aOLIVEIRA, T. C. de A.
245 $aIdentification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil.$h[electronic resource]
260 $c2021
520 $aIn this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.
650 $aBovine mastitis
650 $aMass spectrometry
650 $aBovino
650 $aEspectrometria
650 $aEstafilococo
650 $aEstreptococo
650 $aInfecção
653 $aMastite
653 $aStaphylococci
653 $aStreptococci
700 1 $aBRITO, M. A. P. P.
700 1 $aMARVAL, M. G. de
700 1 $aVICENTINI, N. M.
700 1 $aLANGE, C. C.
773 $tJournal of Dairy Research$gv. 88, p. 302-306, 2021.
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Embrapa Gado de Leite (CNPGL) |
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