02008naa a2200301 a 450000100080000000500110000800800410001902400520006010000260011224501160013826000090025452011370026365000200140065000220142065000110144265000190145365000170147265000170148965000150150665300120152165300180153365300170155170000230156870000210159170000210161270000170163377300560165021351512021-10-07       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1017/ S00220299210005952DOI1 aOLIVEIRA, T. C. de A.  aIdentification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil.h[electronic resource]  c2021  aIn this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.  aBovine mastitis  aMass spectrometry  aBovino  aEspectrometria  aEstafilococo  aEstreptococo  aInfecção  aMastite  aStaphylococci  aStreptococci1 aBRITO, M. A. P. P.1 aMARVAL, M. G. de1 aVICENTINI, N. M.1 aLANGE, C. C.  tJournal of Dairy Researchgv. 88, p. 302-306, 2021.