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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
18/11/2019 |
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Data da última atualização: |
11/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHAIBUB, A. A.; SOUSA, T. P. de; ARAÚJO, L. G. de; FILIPPI, M. C. C. de. |
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Afiliação: |
AMANDA ABDALLAH CHAIBUB, UNB; THATYANE PEREIRA DE SOUSA, UFG; LEILA GARCÊS DE ARAÚJO, UFG; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
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Título: |
Cladosporium cladosporioides C24G modulates gene expression and enzymatic activity during leaf blast suppression in rice plants. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Plant Growth Regulation, v. 39, n. 3, p. 1140-1152, Sept. 2020. |
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Série: |
0721-7595 |
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ISSN: |
1435-8107 |
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DOI: |
https://doi.org/10.1007/s00344-019-10052-9 |
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Idioma: |
Inglês |
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Conteúdo: |
The inclusion of biological control in the integrated management of rice blast (Magnaporthe oryzae) is an alternative to reduce pesticides application. C24G, classified, as Cladosporium cladosporioides was isolated from the phylloplane of the rice plant, therefore, adapted to natural conditions of the original habitat. This study aimed to compare four application methods of C24G in rice plants to suppress leaf blast together with the increase in enzymatic activity and expression of defense genes. It was conducted by four assays (1: seed and soil, 2: soil drenching, 3: foliar spray pulverization-preventive and 4: foliar spray pulverization-curative) for choosing the best application method. The best methods identified were further investigated for the activity of Chitinase (CHI), B-1,3-Glucanase (GLU), Lipoxygenase (LOX), Phenylalanine ammonia-lyase (PAL), and Peroxidase (POX) and the expression of Gns1, JIOsPR10, LOX-RLL, and PR1b genes by Real-time PCR. The preventive foliar spray pulverization suppressed up to 83.78% of leaf blast severity, increasing enzymes (CHI, GLU, LOX, and PAL) activity and genes (JIOsPR10, LOX-RLL, and PR1b) expression. We conclude that Cladosporium cladosporioides isolated C24G is a potential biological agent. To prove its potential as a component of sustainable blast management, it should be tested under field conditions. The application of C24G isolate in rice fields can reduce the number of fungicides spraying, generating greater rentability and decreasing environmental contaminations. MenosThe inclusion of biological control in the integrated management of rice blast (Magnaporthe oryzae) is an alternative to reduce pesticides application. C24G, classified, as Cladosporium cladosporioides was isolated from the phylloplane of the rice plant, therefore, adapted to natural conditions of the original habitat. This study aimed to compare four application methods of C24G in rice plants to suppress leaf blast together with the increase in enzymatic activity and expression of defense genes. It was conducted by four assays (1: seed and soil, 2: soil drenching, 3: foliar spray pulverization-preventive and 4: foliar spray pulverization-curative) for choosing the best application method. The best methods identified were further investigated for the activity of Chitinase (CHI), B-1,3-Glucanase (GLU), Lipoxygenase (LOX), Phenylalanine ammonia-lyase (PAL), and Peroxidase (POX) and the expression of Gns1, JIOsPR10, LOX-RLL, and PR1b genes by Real-time PCR. The preventive foliar spray pulverization suppressed up to 83.78% of leaf blast severity, increasing enzymes (CHI, GLU, LOX, and PAL) activity and genes (JIOsPR10, LOX-RLL, and PR1b) expression. We conclude that Cladosporium cladosporioides isolated C24G is a potential biological agent. To prove its potential as a component of sustainable blast management, it should be tested under field conditions. The application of C24G isolate in rice fields can reduce the number of fungicides spraying, generating greater rentability and... Mostrar Tudo |
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Thesagro: |
Arroz; Atividade Enzimática; Mancha de Cladosporium; Oryza Sativa. |
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Thesaurus Nal: |
Blast disease; Cladosporium cladosporioides; Induced resistance; Magnaporthe oryzae; Rice. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02503naa a2200301 a 4500
001 2114465
005 2020-09-11
008 2020 bl uuuu u00u1 u #d
022 $a1435-8107
024 7 $ahttps://doi.org/10.1007/s00344-019-10052-9$2DOI
100 1 $aCHAIBUB, A. A.
245 $aCladosporium cladosporioides C24G modulates gene expression and enzymatic activity during leaf blast suppression in rice plants.$h[electronic resource]
260 $c2020
490 $a0721-7595
520 $aThe inclusion of biological control in the integrated management of rice blast (Magnaporthe oryzae) is an alternative to reduce pesticides application. C24G, classified, as Cladosporium cladosporioides was isolated from the phylloplane of the rice plant, therefore, adapted to natural conditions of the original habitat. This study aimed to compare four application methods of C24G in rice plants to suppress leaf blast together with the increase in enzymatic activity and expression of defense genes. It was conducted by four assays (1: seed and soil, 2: soil drenching, 3: foliar spray pulverization-preventive and 4: foliar spray pulverization-curative) for choosing the best application method. The best methods identified were further investigated for the activity of Chitinase (CHI), B-1,3-Glucanase (GLU), Lipoxygenase (LOX), Phenylalanine ammonia-lyase (PAL), and Peroxidase (POX) and the expression of Gns1, JIOsPR10, LOX-RLL, and PR1b genes by Real-time PCR. The preventive foliar spray pulverization suppressed up to 83.78% of leaf blast severity, increasing enzymes (CHI, GLU, LOX, and PAL) activity and genes (JIOsPR10, LOX-RLL, and PR1b) expression. We conclude that Cladosporium cladosporioides isolated C24G is a potential biological agent. To prove its potential as a component of sustainable blast management, it should be tested under field conditions. The application of C24G isolate in rice fields can reduce the number of fungicides spraying, generating greater rentability and decreasing environmental contaminations.
650 $aBlast disease
650 $aCladosporium cladosporioides
650 $aInduced resistance
650 $aMagnaporthe oryzae
650 $aRice
650 $aArroz
650 $aAtividade Enzimática
650 $aMancha de Cladosporium
650 $aOryza Sativa
700 1 $aSOUSA, T. P. de
700 1 $aARAÚJO, L. G. de
700 1 $aFILIPPI, M. C. C. de
773 $tJournal of Plant Growth Regulation$gv. 39, n. 3, p. 1140-1152, Sept. 2020.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Tipo/Formato |
Classificação |
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Registro |
Volume |
Status |
URL |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/01/2020 |
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Data da última atualização: |
27/06/2023 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
SÁ, J. F. O. de; BORGES, M. de F.; MARTINS, M. F. |
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Afiliação: |
Jaqueline Flaviana Oliveira de Sá, EPAMIG; Maria de Fatima Borges; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Análises microbiológicas. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: COSTA, R. G. B.; MARTINS, M. F.; MENDONÇA, J. F. M. DE; BORGES, M. DE F. (ed.). Controle de qualidade em queijo minas padrão: métodos físico-químicos, microbiológicos e moleculares. Brasília, DF: Embrapa, 2019. |
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Páginas: |
p. 49-70. |
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Idioma: |
Português |
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Conteúdo: |
Neste capítulo estão descritas as análises exigidas pela legislação brasileira para o queijo minas padrão - coliformes totais e termotolerantes, Staphylococcus coagulase positivo e Salmonella sp. - e também a pesquisa de outros patógenos, como Staphylococcus aureus, Listeria monocytogenes e Escherichia coli O157:H7, comumente envolvidos em surtos de toxinfecções alimentares no mundo. |
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Palavras-Chave: |
Análise de coliformes termotolerantes; Análise de coliformes totais; Análise de Salmonella sp; Análise de Staphylococcus coagulase positivo; Detecção de Escherichia coli; Detecção de Listeria monocytogenes; Detecção de Staphylococcus aureus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01341naa a2200241 a 4500
001 2119292
005 2023-06-27
008 2019 bl uuuu u00u1 u #d
100 1 $aSÁ, J. F. O. de
245 $aAnálises microbiológicas.$h[electronic resource]
260 $c2019
300 $ap. 49-70.
520 $aNeste capítulo estão descritas as análises exigidas pela legislação brasileira para o queijo minas padrão - coliformes totais e termotolerantes, Staphylococcus coagulase positivo e Salmonella sp. - e também a pesquisa de outros patógenos, como Staphylococcus aureus, Listeria monocytogenes e Escherichia coli O157:H7, comumente envolvidos em surtos de toxinfecções alimentares no mundo.
653 $aAnálise de coliformes termotolerantes
653 $aAnálise de coliformes totais
653 $aAnálise de Salmonella sp
653 $aAnálise de Staphylococcus coagulase positivo
653 $aDetecção de Escherichia coli
653 $aDetecção de Listeria monocytogenes
653 $aDetecção de Staphylococcus aureus
700 1 $aBORGES, M. de F.
700 1 $aMARTINS, M. F.
773 $tIn: COSTA, R. G. B.; MARTINS, M. F.; MENDONÇA, J. F. M. DE; BORGES, M. DE F. (ed.). Controle de qualidade em queijo minas padrão: métodos físico-químicos, microbiológicos e moleculares. Brasília, DF: Embrapa, 2019.
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Embrapa Gado de Leite (CNPGL) |
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